Ameliorative effect of curcumin and ascorbic acid against ultraviolet B radiation-induced thyroid toxicity in female Wistar rats: A haematological and biochemical study

The present study aimed to investigate the ameliorative effect of curcumin and ascorbic acid against ultraviolet (UVB)-induced thyroid toxicity and to study the haemtological and biochemical parameters. Twenty-four female Wistar rats, aged 3-4 months and weighing 130-150 g, were used, and the rats were divided into four Groups (Groups I to IV). Group I received standard food and water ad libitum and was treated as a control; Group II received a dose of 280 nm of UVB radiation for 2 hrs/day. Group III received 280 nm UVB radiation for 2 hrs/day and received curcumin 25 mg/kg body weight given orally. Group IV received 280 nm of UVB radiation for 2 hrs/day and received ascorbic acid 250 mg/kg body weight given orally. All the treatments were consequently performed for 15 days. The results showed that haemtological parameters such as haemoglobin (Hb) (p<0.05), red blood cells (RBCs), white blood cells (WBCs), MCV, MCH, and MCHC decreased significantly. Biochemical parameters included lipid peroxidation (LPO) (p<0.05), H2O2 (p<0.01), nitric oxide (NO), superoxide dismutase (SOD) (p< 0.01), catalase (p<0.01), glutathione-S-transferase (GST) (p<0.01), and glutathione reductase. NO increased, and glutathione (GSH) (p<0.01) decreased significantly. However, cotreatment with curcumin and ascorbic acid significantly increased the haemtological parameters. In addition, oxidative parameters such as LPO (p<0.01), SOD (p< 0.01), CAT (p<0.01), GST (p<0.01), and NO (p<0.01) significantly increased, and GSH (p<0.01) significantly decreased upon cotreatment with curcumin and ascorbic acid. The results indicated the ameliorative effect of curcumin and ascorbic acid against UVB-induced thyroid toxicity in female Wistar rats

Research Journal of Pharmaceutical, Biological and Chemical Sciences Production, optimization and characterization of broad spectrum bacteriocins from Lactobacillus plantarum DP2 and Lactobacillus casai DD1

ABSTRACT Two broad spectrum bacteriocins producing lactobacilli isolated from dairy samples were identified as L. plantarum DP2 and L. casai DD1. Bacteriocin production from L. plantarum DP2 was recorded maximum in the presence of maltose as sole source of carbon, whereas glucose was found to be best carbon source in case of L. casai DD1. Both bacteriocin showed maximum production when three nitrogen sources tryptone, yeast extract and meat extract were present together in the medium at pH 6 and 37 o C. Bacteriocins from L. plantarum DP2 and L. casai DD1 were purified up to 4 fold and 6.3 fold with a recovery of 146.8% and 130.2% respectively. Molecular weights of purified bacteriocins were 4.8 kDa (L. plantarum DP2) and 9.2 kDa (L. casai DD1). Bacteriocin from L. plantarum DP2 was thermal stable, active at pH 4 to 8 and also showed stability at high salt concentrations (2-10%). Increased activities of this bacteriocin were also recorded with 2% NaCl, 1% EDTA and 1% tween 80. Whereas bacteriocin produced by L. casai DD1 was heat unstable and also unstable at high salt concentrations. However, both bacteriocins were completely inactivated by proteolytic enzymes

Fabricating a rapid and low-cost electrochemical biosensor with imprints of glycated albumin molecules to detect diabetes using bimetallic Au-Pt nanoparticles on μSPE

Diabetes is a primary factor that is accountable for health problems and results in millions of deaths. 4 million deaths approximately occurred due to diabetes every year and about 170 million people are suffering universally. For diabetes, no treatment is present that promises to cure this condition. Only blood glucose monitoring is recommended for diabetics. Regular and close monitoring of glucose levels can help to avoid further serious problems. Determining the level of glucose in the blood is an important requirement. This is done by monitoring the levels of glycated hemoglobin (HbA1c), which is a biomarker for glucose. But HbA1c monitoring is unreliable in the presence of certain medical conditions. In such cases another biomarker glycated albumin (GA) is used for glucose monitoring as it remains unaffected by such conditions. As a result, a bimetallic nanomaterial-based sensitive platform was created. This research focuses on the development of a biosensor that utilizes micro-screen printed electrodes (μSPE). The platform was designed to measure the level of GA and demonstrated the synergistic effects of bi-metallic gold-platinum (Au-Pt) nanomaterial. Additionally, the electrochemical response of mono-metallic and bi-metallic nanoparticles was investigated in order to detect glycated albumin, a diabetes biomarker. The biosensor constructed using bimetallic nanoparticles exhibits a broad range of concentrations, lower limit of detection, heightened sensitivity, and selectivity