22 research outputs found
Perubahan Kanji dan Gula dalam Proses Kematangan Buah-buahan.
The content of starch and total SU/lars in bananas, papayas and oranges at different ages and stages
of maturity have been determined. In all the fruits studied, the starch content was found to be high in
y·ounger fr~its and then began to decrease as the fruits matured and ripened. Comparing all the three types
of fruits studied, bananas seemed to have the highest starch content followed by papayas and oranges.
The levels of sugars, on the other hand, were initially low, then continue to increase until the ripening stage.
Papayas (particularly the yellow flesh) appeared to have the highest concentration of sugars, followed
by Pisang Berangan, oranges and Pisang Nangka. The rates of starch and sugar changes varied with fruit
types and varietie
Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.
The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus
angustifolius could be partially or fully restored by the addition of divalent cations to the incubation
mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; followed
by Zn2+ (59%), Co2+ (55%), Mg2+ (53%) and Cu 2
+ (49%). The Mn-deficient enzyme was more sensitive to
manganese and more stimulated at a lower manganese concentration than the Mn-sufficient enzyme.
However, Mn-deficient enzyme achieved a maximum activity of 91% at 1.4mM Mn2+ while Mn-sufficient
enzyme achieved a maximum activity of 105% of control at 2.0mM
Properties of glutamate dehydrogenase in developing legume fruit. I. The effects of chelating agents
A study was conducted to investigate the effects of EDTA. and Chelex-lOO on the glutamate
dehydrogenase activity from a crude extract of the developing seeds of Lupinus angustifolius. The enzyme
was not inhibited by Chelex-lOO; instead it was highly stimulated and its activity, when treated with
Chelex-lOO, was higher than that of the control. Glutamate dehydrogenase, however, was inhibited by
5mM EDTA, stimulated at 30mM EDTA, and inhibited again at 45mM EDTA
Growth Characteristics and Production of Physalins from Physalis minima Hairy Roots in Shake Flasks
This report described the growth characteristics and production of physalins from Physalis
minima hairy roots in shake fl ask culture. The presence of lateral branches in the inoculum had a
negligible effect on the fi nal root biomass dry weight (DW), specifi c growth rate (μ), doubling time (td)
and production of physalins. However, excising the primary and lateral root tips reduced μ and the total
root length but not the fi nal biomass. Mature root tissues were observed to accumulate more physalin
B and F (1.55 and 3.74 mg.g-1 DW, respectively) compared to the root tips (0.65 and 1.47 mg.g-1 DW,
respectively). Increasing the number of root tips from 2 to 12 and the medium volume signifi cantly
reduced μ and extended td. Decreasing the medium volume with a small number of inocula reduced td,
improved the biomass and production of physalins and μ. Using a 100 mL fl ask, four root tips cultured in
25 mL medium provided the optimum conditions for biomass (0.24 g DW) and production of physalins
(1.68–3.5 mg.g-1 DW)