Prescription pattern of fixed dose drug combinations in obstetrics and gynecology department of a tertiary care hospital in Puducherry, India: an observational study

Background: Fixed drug combinations (FDCs)have various advantages and disadvantages. In countries like India there are numerous irrational prescriptions as highlighted by the recent banning of FDCs in October 2018. Studying the prescription pattern helps in developing national database which can be used to promote rational use of drugs.Methods: All the Outdoor Patient Department (OPD) prescriptions from department of Obstetrics and Gynecology (OBG) during the study period were used for the study. The drugs were classified according to Anatomical Therapeutic Chemical (ATC) classification. Other data studied were the number of FDCs and the number of currently banned combinations which were used during the study period.Results: The 41% of the drugs prescribed as FDCs. Most FDCs belonged to alimentary system followed by anti-infectives and blood and blood forming organs group. Vitamin D3 and Calcium combination was the most commonly prescribed FDC. Approximately 20% of these prescribed drugs are currently banned.Conclusions: A significant number of drugs are being prescribed as FDCs which also includes various irrational combinations

Isolation and culture of protoplasts of Capsicum annuum L. and their regeneration into plants flowering in vitro

Axenic shoot cultures of Capsicum annuum cv. California Wonder were used as the source for isolation of protoplasts from mesophyll cells. Protoplasts underwent sustained mitotic activity and proliferated to form callus masses on NT or DPD medium enriched with 2,4-D, NAA and BAP each at 1 mg/l level. The callus could be differentiated into whole plants on the differentiation media and plants floweredin vitro under long day conditions

Plantlet formation from isolated protoplasts of Solanum melongena L

Mesophyll protoplasts were isolated from axenic shoot cultures of Solanum melongena by the one-step enzymatic method. Of the different media employed for the culture of protoplasts, a medium modified from Kao and Michayluk (1975) supported sustained mitotic cycles most effectively. Organogenesis from protoplast-derived callus was achieved on transfer to Murashige and Skoog'S (1962) medium supplemented with an appropriate auxin and a cytokinin

Plantlets from mesophyll protoplasts of Solanum xanthocarpum

Young leaves of Solanum xanthocarpum from axenic shoot cultures released viable protoplasts when treated with appropriate enzymes. The protoplasts on culture in modified Murashige and Skoog (1962) medium supplemented with 2,4-dichlorophenoxy-acetic acid (0.5 mg/l), naphtha leneacetic acid (1 mg/l), kinetin (1 mg/l) and organic nutrients of KM (Kao and Michayluk 1975) regenerated to form callus tissue as a result of repeated divisions. Protoplast-derived calli differentiated into shoots on MS medium enriched with kinetin (0.5 mg/l) and rooting could be initiated by transferring the shoot-buds to basal medium

Study of prevalence of osteoporosis in males of above 40 years age group attending SMS Hospital, Jaipur

Background: Whenever osteoporosis is discussed, the focus is on women; men are far less likely to receive a diagnosis of osteoporosis or osteoporotic fracture because of considerable gaps in knowledge on male osteoporosis. The aim and objectives were to study the prevalence of osteoporosis in males of above 40 year age group attending SMS Hospital Jaipur & to explore the influence of various modifiable and non-modifiable risk factors on BMD.Methods: Study Location: SMS Medical College and Hospital, Jaipur. Study design: Hospital based cross sectional study. Study period: April 2015 to December 2016. Sample Size: 200. Work up: After taking ethical clearance and informed verbal consent, demographic and clinical details were noted along with S- calcium, Vitamin D and bone mineral density assessment. Osteoporosis was defined as T score ≤−2.5 bone mass −1 to −2.5 and normal as >−1. Data thus collected was analysed with help of SPSS 22.0 through frequency, percentages, Mean, SD and ANOVA.Results: Prevalence of osteopenia and osteoporosis in the study population was 28.5% and 11.5%. Age wise maximum prevalence was in the age group 71-80 years (31.81%). Prevalence of osteoporosis was more among Muslim community 20.83%, more in low socio economic group (BPL). T score of study population was -0.3705±1.41. The mean BMI, S-Calcium, Vitamin D levels and T score values among osteopenic and osteoporotic patients were statistically highly significant when compared to patients without osteo-penic/porotic changes (p<0.05).Conclusions: Osteoporosis is a silent killer and prevention is better than cure as prevention requires simple steps such as good dietary habits, active life style, good control of systemic disorders, reduced intake of tobacco and alcohol

Formulation Development and Evaluation of Aqueous Injection of Poorly Soluble Drug Made by Novel Application of Mixed Solvency Concept

It is commonly recognized in the pharmaceutical industry that on average more than 40% of newly discovered drug candidates are poorly water-soluble. The objective of present research is to explore the application of mixed solvency technique in the injection formulation of poorly soluble drugs and to reduce concentration of individual solubilizers (used for solubility enhancement) to minimize the toxic effects of solubilizers. In the present work poorly soluble drugs Ofloxacin are selected as model drugs. Ofloxacin is an antibiotic drug tried to formulate the aqueous injection by the use of various physiologically compatible solubilizing agent like Lignocaine Hydrochloride, Niacinamide, Sodium benzoate, Sodium citrate, PEG 400, PEG 4000, PVP 40000, Ethanol, and Propylene Glycol. For expected synergistic enhancement effect on solubility of these poorly soluble drugs various blends of solubilizers shall be tried to decrease the amounts of Solubilizer employed for a desired solubility enhancement ratio. The study further opens the chances of preparing dry powders for injection of drug which are not stable in aqueous solution, ready to use injection. Key word- Mixed solvency solubilization, Ofloxacin, solubility enhancement, synergistic enhancement effect

Respon Fisiologi Domba pada Sistem Pemeliharaan dan Pemberian Jenis Konsentrat Berbeda

Sheep is one of the livestock commodities that is developed and utilized by farmer to meet the increasing demand of animal protein. This study aimed to investigate physiological responses of local sheep that were kept on different raising system and given different type concentrate. The study used 24 heads of local sheep with an average initial live weight of 19.79±2.47kg. The experiment was set up in a completely randomized design with two factors. The first factor was rearing system (intensive and semi intensive) and second factor was type of concentrate feed (green and comercial concentrates). The parameters observed were heart rate, rectal temperature, respiration rate, differential leukocytes N/L and cortisol hormone levels. The data were analyzed by repeated mensure analysis of variance (repeted ANOVA). The results showed that the type of concentrate did not affect blood profile and cortisol hormone levels. Sheep’s physiological response (respiration rate, heart rate and rectal temperature) were significantly (P0 <0.5) influenced by rearing system