Antibiotic resistance pattern of Klebsiella pneumoniae isolated from nosocomial infections in Aleshtar hospital, Lorestan province

Introduction: Klebsiella pneumoniae is one of Klebsiella species. K. pneumoniae is one of the most important bacteria causing nosocomial infections. This bacterium threatens public health and leads to increased hospital costs and mortality rate. The purpose of this study was to determine the pattern of antibiotic resistance in K. pneumoniae in nosocomial infections.Methods: This study was performed on 51 samples of Klebsiella isolates from 500 patients in three units of Aleshtar hospital in 9 months. The antibiotic resistance of K. pneumoniae to 18 antibiotics was performed by Kirby Bauer disk diffusion method.Results: The frequency of K. pneumonia among 500 samples was determined 51 cases (10.2%). The largest number of K. pneumoniae was isolated from the infectious unit (49.02%). The frequency of K. pneumoniae based on the source of infection for urine was 22 cases (43.14%), sputum 17 (33.33%), stool 6 (11.77%), wound 4 (7.84%), blood 2 (3.92%), and cerebrospinal fluid 0 (0%), respectively. K. pneumoniae resistance to antibiotics included: ceftriaxone (94.12%), ciprofloxacin (90.20%), ofloxacin (86.27%), cefotaxime (78.43%), nalidixic acid (58.82%), nitrofurantoin (56.86%), aztreonam (54.90%), ampicillin/sulbactam (50.98% ), ticarcillin (45.09%), cefepime (43.14%), colistin (43.14%), gentamicin (41.18%), azithromycin (41.18%), polymyxin B (39.22%), piperacillin/tazobactam (19.61%), amikacin ( 15.69%), imipenem (5.88%), and meropenem (3.92%).Conclusion: Meropenem and imipenem with the lowest resistance were the most effective antibiotics against K .pneumoniae. Ceftriaxone, and ciprofloxacin antibiotic had the lowest effect on the treatment of K. pneumoniae

Quantitative Proteomic Analysis of Human Embryonic Stem Cell Differentiation by 8-Plex iTRAQ Labelling

Analysis of gene expression to define molecular mechanisms and pathways involved in human embryonic stem cells (hESCs) proliferation and differentiations has allowed for further deciphering of the self-renewal and pluripotency characteristics of hESC. Proteins associated with hESCs were discovered through isobaric tags for relative and absolute quantification (iTRAQ). Undifferentiated hESCs and hESCs in different stages of spontaneous differentiation by embryoid body (EB) formation were analyzed. Using the iTRAQ approach, we identified 156 differentially expressed proteins involved in cell proliferation, apoptosis, transcription, translation, mRNA processing, and protein synthesis. Proteins involved in nucleic acid binding, protein synthesis, and integrin signaling were downregulated during differentiation, whereas cytoskeleton proteins were upregulated. The present findings added insight to our understanding of the mechanisms involved in hESC proliferation and differentiation

Clinical Breast Cancer Registry of IR. Iran (CBCR-IR):Study Protocol and First Results

BACKGROUND: Breast cancer (BC), as a significant global health problem, is the most common cancer in women. Despite the importance of clinical cancer registries in improving the quality of cancer care and cancer research, there are few reports on them from low- and middle-income countries. We established a multicenter clinical breast cancer registry in Iran (CBCR-IR) to collect data on BC cases, the pattern of care, and the quality-of-care indicators in different hospitals across the country.METHODS: We established a clinical cancer registry in 12 provinces of Iran. We defined the organizational structure, developed minimal data sets and data dictionaries, verified data sources and registration processes, and developed the necessary registry software. During this registry, we studied the clinical characteristics and outcomes of patients with cancer who were admitted from 2014 onwards.RESULTS: We registered 13086 BC cases (7874 eligible cases) between 1.1.2014 and 1.1.2022. Core needle biopsy from the tumor (61.25%) and diagnostic mammography (68.78%) were the two most commonly used diagnostic methods. Stage distribution was 2.03% carcinoma in situ, 12% stage I, 44.65% stage II, 21.32% stage III, and 4.61% stage IV; stage information was missing in 1532 patients (19.46%). Surgery (95.01%) and chemotherapy (79.65%) were the most common treatments for all patients.CONCLUSION: The information provided by this registry can be used to evaluate and improve the quality of care for BC patients. It will be scaled up to the national level as an important resource for measuring quality of care and conducting clinical cancer research in Iran.</p

The functional analysis of 6 k-mean clusters.

<p>This list of proteins was employed to identify significantly activated pathways by comparing their functional annotations according to the PANTHER classification systems. Proteins involved in nucleic acid binding, protein synthesis, and signaling (particularly, proteins involved in integrin signaling) were enriched in the downregulated protein clusters (clusters 1, 2, and 3), whereas the upregulated proteins in clusters 4, 5, and 6 were enriched in proteins involved in cytoskeleton structure.</p

Correlation between the expression patterns of differentially expressed proteins identified in this study and their corresponding mRNA at three different stages.

<p>Correlation between the expression patterns of differentially expressed proteins identified in this study and their corresponding mRNA at three different stages.</p

Clinical Breast Cancer Registry of IR. Iran (CBCR-IR):Study Protocol and First Results

BACKGROUND: Breast cancer (BC), as a significant global health problem, is the most common cancer in women. Despite the importance of clinical cancer registries in improving the quality of cancer care and cancer research, there are few reports on them from low- and middle-income countries. We established a multicenter clinical breast cancer registry in Iran (CBCR-IR) to collect data on BC cases, the pattern of care, and the quality-of-care indicators in different hospitals across the country.METHODS: We established a clinical cancer registry in 12 provinces of Iran. We defined the organizational structure, developed minimal data sets and data dictionaries, verified data sources and registration processes, and developed the necessary registry software. During this registry, we studied the clinical characteristics and outcomes of patients with cancer who were admitted from 2014 onwards.RESULTS: We registered 13086 BC cases (7874 eligible cases) between 1.1.2014 and 1.1.2022. Core needle biopsy from the tumor (61.25%) and diagnostic mammography (68.78%) were the two most commonly used diagnostic methods. Stage distribution was 2.03% carcinoma in situ, 12% stage I, 44.65% stage II, 21.32% stage III, and 4.61% stage IV; stage information was missing in 1532 patients (19.46%). Surgery (95.01%) and chemotherapy (79.65%) were the most common treatments for all patients.CONCLUSION: The information provided by this registry can be used to evaluate and improve the quality of care for BC patients. It will be scaled up to the national level as an important resource for measuring quality of care and conducting clinical cancer research in Iran.</p

Experimental design of proteome analysis of hESCs using iTRAQ labeling.

<p>Samples from undifferentiated hESCs and EBs at days 6, 12, and 20 were collected in three biological replicates. Similar amounts of proteins were digested into peptides using trypsin. Peptides were subsequently desalted and labeled with 8-plex iTRAQ reagents 113–121. Labeled peptides were pooled, fractionated into 20 SCX fractions, and then analyzed by reverse-phase LC-MS/MS.</p

A Fresh look at the male-specific region of the human Y chromosome

The Chromosome-centric Human Proteome Project (C-HPP) aims to systematically map the entire human proteome with the intent to enhance our understanding of human biology at the cellular level. This project attempts simultaneously to establish a sound basis for the development of diagnostic, prognostic, therapeutic, and preventive medical applications. In Iran, current efforts focus on mapping the proteome of the human Y chromosome. The male-specific region of the Y chromosome (MSY) is unique in many aspects and comprises 95% of the chromosome's length. The MSY continually retains its haploid state and is full of repeated sequences. It is responsible for important biological roles such as sex determination and male fertility. Here, we present the most recent update of MSY protein-encoding genes and their association with various traits and diseases including sex determination and reversal, spermatogenesis and male infertility, cancers such as prostate cancers, sex-specific effects on the brain and behavior, and graft-versus-host disease. We also present information available from RNA sequencing, protein-protein interaction, post-translational modification of MSY protein-coding genes and their implications in biological systems. An overview of Human Y chromosome Proteome Project is presented and a systematic approach is suggested to ensure that at least one of each predicted protein-coding gene's major representative proteins will be characterized in the context of its major anatomical sites of expression, its abundance, and its functional relevance in a biological and/or medical context. There are many technical and biological issues that will need to be overcome in order to accomplish the full scale mapping.17 page(s

A Fresh Look at the Male-specific Region of the Human Y Chromosome

The Chromosome-centric Human Proteome Project (C-HPP) aims to systematically map the entire human proteome with the intent to enhance our understanding of human biology at the cellular level. This project attempts simultaneously to establish a sound basis for the development of diagnostic, prognostic, therapeutic, and preventive medical applications. In Iran, current efforts focus on mapping the proteome of the human Y chromosome. The male-specific region of the Y chromosome (MSY) is unique in many aspects and comprises 95% of the chromosome’s length. The MSY continually retains its haploid state and is full of repeated sequences. It is responsible for important biological roles such as sex determination and male fertility. Here, we present the most recent update of MSY protein-encoding genes and their association with various traits and diseases including sex determination and reversal, spermatogenesis and male infertility, cancers such as prostate cancers, sex-specific effects on the brain and behavior, and graft-versus-host disease. We also present information available from RNA sequencing, protein–protein interaction, post-translational modification of MSY protein-coding genes and their implications in biological systems. An overview of Human Y chromosome Proteome Project is presented and a systematic approach is suggested to ensure that at least one of each predicted protein-coding gene's major representative proteins will be characterized in the context of its major anatomical sites of expression, its abundance, and its functional relevance in a biological and/or medical context. There are many technical and biological issues that will need to be overcome in order to accomplish the full scale mapping