122 research outputs found
Survey Sequencing and Comparative Analysis of the Elephant Shark (Callorhinchus milii) Genome
Owing to their phylogenetic position, cartilaginous fishes (sharks, rays, skates, and chimaeras) provide a critical reference for our understanding of vertebrate genome evolution. The relatively small genome of the elephant shark, Callorhinchus milii, a chimaera, makes it an attractive model cartilaginous fish genome for whole-genome sequencing and comparative analysis. Here, the authors describe survey sequencing (1.4× coverage) and comparative analysis of the elephant shark genome, one of the first cartilaginous fish genomes to be sequenced to this depth. Repetitive sequences, represented mainly by a novel family of short interspersed element–like and long interspersed element–like sequences, account for about 28% of the elephant shark genome. Fragments of approximately 15,000 elephant shark genes reveal specific examples of genes that have been lost differentially during the evolution of tetrapod and teleost fish lineages. Interestingly, the degree of conserved synteny and conserved sequences between the human and elephant shark genomes are higher than that between human and teleost fish genomes. Elephant shark contains putative four Hox clusters indicating that, unlike teleost fish genomes, the elephant shark genome has not experienced an additional whole-genome duplication. These findings underscore the importance of the elephant shark as a critical reference vertebrate genome for comparative analysis of the human and other vertebrate genomes. This study also demonstrates that a survey-sequencing approach can be applied productively for comparative analysis of distantly related vertebrate genomes
Molecular Characterization of Branchial aquaporin 1aa and Effects of Seawater Acclimation, Emersion or Ammonia Exposure on Its mRNA Expression in the Gills, Gut, Kidney and Skin of the Freshwater Climbing Perch, Anabas testudineus
10.1371/journal.pone.0061163PLoS ONE84
Basics of Bose-Einstein Condensation
The review is devoted to the elucidation of the basic problems arising in the
theoretical investigation of systems with Bose-Einstein condensate.
Understanding these challenging problems is necessary for the correct
description of Bose-condensed systems. The principal problems considered in the
review are as follows: (i) What is the relation between Bose-Einstein
condensation and global gauge symmetry breaking? (ii) How to resolve the
Hohenberg-Martin dilemma of conserving versus gapless theories? (iii) How to
describe Bose-condensed systems in strong spatially random potentials? (iv)
Whether thermodynamically anomalous fluctuations in Bose systems are
admissible? (v) How to create nonground-state condensates? Detailed answers to
these questions are given in the review. As examples of nonequilibrium
condensates, three cases are described: coherent modes, turbulent superfluids,
and heterophase fluids.Comment: Review articl
Immunocytochemical localization of carbonic anhydrase in the pseudobranch tissue of the rainbow trout Oncorhynchus my-kiss
Microscopical methods for the localization of Na + , K + -ATPase
Na + , K + -ATPase plays a central role in the ionic and osmotic homeostasis of cells and in the movements of electrolytes and water across epithelial boundaries. Microscopic localization of the enzyme is, therefore, of crucial importance in establishing the subcellular routes of electrolyte flow across structurally complex and functionally polarized epithelia. Recently developed approaches to the localization of Na + , K + -ATPase are reviewed. These methods rely on different properties of the enzyme and encompass cytochemical localization of the K + -dependent nitrophenylphosphatase component of the enzyme, autoradiographic localization of tritiated ouabain binding sites, and immunocytochemical localization of the holoenzyme and of its catalytic subunit. The rationales for each of these techniques are outlined as are the critieria that have been established to validate each method. The observed localization of Na + , K + -ATPase in various tissues is discussed, particularly as it relates to putative and hypothetical mechanisms that are currently thought to mediate reabsorptive and secretory electrolyte transport.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42850/1/10735_2005_Article_BF01005056.pd
Acute response to a salt load of the NaCl excretion mechanisms of the gill ofPlatichthys flesus in sea water
The effects of various external cations and sodium transport inhibitors on sodium uptake by the sailfin molly,Poecilia latipinna, acclimated to sea water
Factors affecting the net fluxes of ions in the isolated perfused gills of freshwaterAnguilla dieffenbachii
A study of the Na+/NH 4 + exchange across the gill of the perfused head of the trout (Salmo gairdneri)
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