Proteome of the aging mice heart

Aging induces pathological cardiovascular changes such as cardiac dysfunction and arteriosclerosis. With aging, heart cells, especially, become more susceptible to lethal damage. In this report, we tried to understand the precise mechanism of myocardial change resulting from aging by examining the heart proteome in aging mice using two-dimensional gel electrophoresis (2DE). The proteins were stained with fluorescence dyes (SYPRO Ruby and Pro-Q Diamond) and identified by subsequent MALDI-TOF-MS / MS. As a result, markedly altered levels of 14 proteins and 7 phosphoproteins were detected in the hearts of 3-, 7-, 11-, and 20-month-old mice. The functions of these identified proteins and phosphoproteins were energy metabolism, muscle contraction, glycolysis, and cytoskeletal support. Additionally, the results of Western blotting confirmed changes in the expression of FTH, CPNE5, and SUCLA2. These findings showed that aging modified the expression of proteins and phosphoproteins in the heart. We suggest that changes in the expression of these proteins are critical to the development of cardiac dysfunction resulting from aging

Rimklb mutation causes male infertility in mice

Rimklb is a mammalian homologue of the E. coli enzyme RimK, which catalyzes addition of glutamic acid to the ribosomal protein S6. To date, no previous studies have shown any physiological role for Rimklb in mammals. In this study, using Western blotting, we found that Rimklb is distributed and expressed in mouse testis and heart. Rimklb was subsequently localized to the testicular Leydig cells using immunohistochemistry with an anti-Rimklb antibody. We generated a Rimklb mutant mouse in which a three-base deletion results in deletion of Ala 29 and substitution of Leu 30 with Val, which we named the RimklbA29del, L30V mutant mouse. RimklbA29del, L30V mutant mice show a decrease in testicular size and weight, and in vitro fertilization demonstrates complete male infertility. Furthermore, we found that a key factor in the mammalian target of the rapamycin/ribosomal protein S6 transcriptional pathway is hyperphosphorylated in the seminiferous tubules of the mutant testis. We conclude that Rimklb has important roles that include spermatogenesis in seminiferous tubules. In summary, male RimklbA29del, L30V mice are infertile

A patient of non-amyloid monoclonal immunoglobulin deposition disease (MIDD) associated with multiple myeloma who rapidly developed disturbance of consciousness and multiple organ failure

We describe a 69-year old man who developed non-amyloid monoclonal immunoglobulin deposition disease (MIDD) associated with multiple myeloma. His MIDD rapidly developed into disturbance of consciousness and multiple organ failure. Several treatments including of chemotherapy were ineffective, and no improvement in disturbed consciousness was observed and multiple organ failure progressed accompanied by pneumonia, with a outcome fatal. The autopsy revealed the depositions of IgA and λchain were observed in the stoma of the heart, pancreas, lung, liver, spleen, and kidney. These results suggest the major cause of multiple organ failure was considered to be MIDD, and the disturbed consciousness may involve in this diseas

A patient of non-amyloid monoclonal immunoglobulin deposition disease (MIDD) associated with multiple myeloma who rapidly developed disturbance of consciousness and multiple organ failure

We describe a 69-year old man who developed non-amyloid monoclonal immunoglobulin deposition disease (MIDD) associated with multiple myeloma. His MIDD rapidly developed into disturbance of consciousness and multiple organ failure. Several treatments including of chemotherapy were ineffective, and no improvement in disturbed consciousness was observed and multiple organ failure progressed accompanied by pneumonia, with a outcome fatal. The autopsy revealed the depositions of IgA and λchain were observed in the stoma of the heart, pancreas, lung, liver, spleen, and kidney. These results suggest the major cause of multiple organ failure was considered to be MIDD, and the disturbed consciousness may involve in this diseas

[Case Reports] Epstein-Barr virus-associated T-cell lymphoma presenting as intractable gastric ulcers due to the vascular wall invasion 4 months before cervical nodal mass formation

[Abstract] We report here a 75-year-old male in whom fever and gastric perforation due to multiple gastric ulcers developed 4 months before the development of cervical lymph node swelling. At the time of disease onset, he did not show the characteristics patterns of anti-Epstein-Barr virus (EBV) antibody responses with raised antiviral capsid (VCA) and anti-early antigen (EA) antibodies. He was diagnosed with chronic active EBV infection (CAEBV) based on an increased EBV-DNA level in the peripheral blood. Four months later, biopsy specimen of the swollen lymph nodes revealed the infiltration of clonal lymphoma cells positive for CD3, CD8, Tcell-restricted intracellular antigen 1 (TIA-1), and EBV-encoded RNA (EBER). He died of progressive infection with cytomegalovirus and invasive aspergillus and intractable multiple hemorrhagic gastric ulcers. The cause of the multiple gastric ulcers could not be identified before death. Autopsy revealed the infiltration of CD8-positive T cells with the same surface phenotypes as those of the cervical malignant lymphoma to the perivascular areas of the ulcer base and necrotic vasculitis. This case suggested that EBV-associated T-cell lymphoma can cause gastric wall infiltration and organ injury without forming an extranodal mass even 4 months earlier than the formation of nodal lesions.Maekura, Syunnji : Otiai, Ke