Nutrient digestibility and ruminal characteristics of buffaloes and bovine fed additived sugar cane silages

It was evaluated nutrients total digestibility (TD) and dry matter (DM) intake, pH and NH3 ruminal concentration in buffaloes and bovine, fed sugar cane silages treated with Lactobacillus buchneri (SSL), plus 10% - cassava by-product meal (SSLC), plus 10% - soybean hulls (SSLS) or plus 1% - urea (SSLU). The experimental design was a double 4x4 Latin squares with a 4 x 2 factorial arrangement. There was no interaction between species x treatment for evaluated parameters except for TD of CP, NDF and ADF. The soybean hulls addition increased (P<0.05) TD of DM, OM and total carbohydrate (TC) while the urea addition reduced (P<0.05) DM intake and TD of DM, OM, TC and no fiber carbohydrate (NFC). Buffaloes showed the highest (P<0.05) TD of CP with SSL and the highest TD of NDF and ADF with SSLS. Bovines showed the highest (P<0.05) TD of CP with the SSL and SSLS; the last increased TD of NDF and ADF. The rumen pH in buffalo was higher than in cattle (6.62 vs. 6.48, respectively). The NH3 ruminal concentration did not differ between species and SSLU treatment presented the highest values (12.9 mg / 100 mL)

Downstream migration of newly-hatched ayu (Plecoglossus altivelis) in the Tien Yen River of northern Vietnam

The ayu (Plecoglossus altivelis) is an annual, amphidromous, plecoglossid fish, distributed in Vietnam, China, Taiwan, Korea, and Japan. To date, ayu have been found only in two rivers in northern Vietnam, where little is known about their life history. The Tien Yen River is believed to be the most southwestern habitat for this species. To determine whether newly hatched ayu larvae drift and to understand their downstream migration, intensive surveys were conducted in the Tien Yen River from October to March of 2013–2016. In total, 529 drifting ayu larvae were collected from four of six sampling stations along the river. Thus, ayu reproduction has been confirmed in this river for the first time, where only adult fish had been found previously. However, we did not successfully collect larvae in the eastern branch of the river, which has a hydroelectric dam, suggesting that ayu do not inhabit this branch or else do not reproduce there. The presence of drifting larvae in the western branch from mid-December to late January implies that they spawn from late November to mid-January. Drifting larvae were captured primarily at night, but peak occurrences varied depending upon the day and the sampling station. With the range of body sizes and variable diel abundance patterns, ayu in the Tien Yen River probably employ multiple spawning grounds. This study provides fundamental life history data for the vulnerable ayu populations in northern Vietnam

Fitting birth-death processes to panel data with applications to bacterial DNA fingerprinting

Continuous-time linear birth-death-immigration (BDI) processes are frequently used in ecology and epidemiology to model stochastic dynamics of the population of interest. In clinical settings, multiple birth-death processes can describe disease trajectories of individual patients, allowing for estimation of the effects of individual covariates on the birth and death rates of the process. Such estimation is usually accomplished by analyzing patient data collected at unevenly spaced time points, referred to as panel data in the biostatistics literature. Fitting linear BDI processes to panel data is a nontrivial optimization problem because birth and death rates can be functions of many parameters related to the covariates of interest. We propose a novel expectation--maximization (EM) algorithm for fitting linear BDI models with covariates to panel data. We derive a closed-form expression for the joint generating function of some of the BDI process statistics and use this generating function to reduce the E-step of the EM algorithm, as well as calculation of the Fisher information, to one-dimensional integration. This analytical technique yields a computationally efficient and robust optimization algorithm that we implemented in an open-source R package. We apply our method to DNA fingerprinting of Mycobacterium tuberculosis, the causative agent of tuberculosis, to study intrapatient time evolution of IS6110 copy number, a genetic marker frequently used during estimation of epidemiological clusters of Mycobacterium tuberculosis infections. Our analysis reveals previously undocumented differences in IS6110 birth-death rates among three major lineages of Mycobacterium tuberculosis, which has important implications for epidemiologists that use IS6110 for DNA fingerprinting of Mycobacterium tuberculosis.Comment: Published in at http://dx.doi.org/10.1214/13-AOAS673 the Annals of Applied Statistics (http://www.imstat.org/aoas/) by the Institute of Mathematical Statistics (http://www.imstat.org

Empiric treatment of pulmonary TB in the Xpert era: Correspondence of sputum culture, Xpert MTB/RIF, and clinical diagnoses.

BackgroundClinical tuberculosis diagnosis and empiric treatment have traditionally been common among patients with negative bacteriologic test results. Increasing availability of rapid molecular diagnostic tests, including Xpert MTB/RIF and the new Xpert Ultra cartridge, may alter the role of empiric treatment.MethodsWe prospectively enrolled outpatients age &gt; = 15 who were evaluated for pulmonary tuberculosis at three health facilities in Kampala, Uganda. Using sputum mycobacterial culture, interviews, and clinical record abstraction, we estimated the accuracy of clinical diagnosis relative to Xpert and sputum culture and assessed the contribution of clinical diagnosis to case detection.ResultsOver a period of 9 months, 99 patients were diagnosed with pulmonary tuberculosis and subsequently completed sputum culture; they were matched to 196 patients receiving negative tuberculosis evaluations in the same facilities. Xpert was included in the evaluation of 291 (99%) patients. Compared to culture, Xpert had a sensitivity of 92% (95% confidence interval 83-97%) and specificity of 95% (92-98%). Twenty patients with negative Xpert were clinically diagnosed with tuberculosis and subsequently had their culture status determined; two (10%) were culture-positive. Considering all treated patients regardless of Xpert and culture data completeness, and considering treatment initiations before a positive Xpert (N = 4) to be empiric, 26/101 (26%) tuberculosis treatment courses were started empirically. Compared to sputum smear- or Xpert-positive patients with positive cultures, empirically-treated, Xpert-negative patients with negative cultures had higher prevalence of HIV (67% versus 37%), shorter duration of cough (median 4 versus 8 weeks), and lower inflammatory markers (median CRP 7 versus 101 mg/L).ConclusionJudged against sputum culture in a routine care setting of high HIV prevalence, the accuracy of Xpert was high. Clinical judgment identified a small number of additional culture-positive cases, but with poor specificity. Although clinicians should continue to prescribe tuberculosis treatment for Xpert-negative patients whose clinical presentations strongly suggest pulmonary tuberculosis, they should also carefully consider alternative diagnoses

Dental students’ levels of understanding panoramic

Background/purpose: In dentistry, panoramic radiography is an important examination technique. The Faculty of Dentistry at Tokushima University educates students about panoramic radiographic anatomical landmarks. The purpose of this study was to investigate differences in the understanding of each panoramic anatomical landmark among students. Materials and methods: This study analyzed the results of 40 fifth- and 79 sixth-year faculty students who had taken a written examination to clarify their knowledge of anatomical landmarks in 28 panoramic radiographic regions. Anatomical landmarks were classified into 3 categories: , , and to compare correct answer rates. Results: The mean overall correct answer rate by the 119 students for the 28 regions was 53%. The rate did not vary between the 2 academic years. On comparison of the 3 categories, significant differences were only observed between and , as the values for the latter were lower. Among the anatomical landmarks, the rates for the condylar head, hyoid bone, panoramic innominate line, styloid process, and nasopalatine line were higher, and those for ghost images of the contralateral nasopalatine line, the cervical vertebrae, mandibular foramen, mastoid air cell, and posterior pharyngeal wall were lower. The values for , such as the dorsum of tongue, middle and inferior nasal conche, and middle and inferior nasal meatuses, were also lower. Conclusion: These results indicate the necessity of improving educational approaches for regions with lower rates of correct answers

The Development of Japanese City\u27s Future Simulation System: My City Forecast

In recent years, the optimization of urban management due to the rapid population decline has been one of the major issues in Japan. Future population estimation and related statistical information, such as the location information of the public facility is now available to open. However, open data utilization in the urban planning field is not advanced in comparison with other countries. We constructed the Web system using FOSS4G that citizens can be the future image of the city to operate on their own. It used mainly below FOSS4G tools; OpenLayers, PostGIS, Pgrouting and Geocolor. The collected data calculated in advance, and the default mode for displaying a simulation result in the 500m mesh unit. In addition to there is a customized mode to be re-calculated using the geodata that citizen is uploading open geospatial data. In the customization mode, to choose the residence induction region arbitrarily, it is possible to more detailed simulation by inputting the future urban areas. We also held a workshop for Japanese citizens the variety of stakeholders, it was also able to get feedback on the functional requirements.For the ordinary citizens are not familiar with GIS, to be able to display an easy-to-understand the future of the region have been evaluated. However, also revealed that the operation of the customized version feels as difficult to use, such as difficult legend of adjustment

現行の口内法デジタルX線撮影システム間の薄い対象物及び厚い対象物におけるコントラストの違いの評価

The purpose is to evaluate the performance of current intraoral digital detectors in detail using a precise phantom and new method. Two aluminum step wedges in 0.5 mm steps were exposed by two photostimulable phosphor plate (PSP) systems—one with automatic exposure compensation (AEC) and the other without AEC—and a CCD sensor. Images were obtained with 3 doses at 60 kV. The effect of metallic material also was evaluated. The contrast-to-noise ratio (CNR) for thinner steps and the low contrast value (LCV) for thicker steps were obtained. The CCD system was the best under all conditions ( < 0.001), although the Gray value was sensitive to the dose, and the Gray value-dose relation varied greatly. The PSP system with AEC was superior to that without AEC for the LCV ( < 0.001) but was inferior to it regarding the CNR ( < 0.001). CNR and LCV in the PSP system without AEC were not affected by the metallic plate. Intraoral digital imaging systems should be chosen according to their diagnostic purpose. PSP system with AEC may be the best for detecting molar proximal caries, whereas the PSP system without AEC may be better for evaluating small bone regeneration in periodontal disease. The CCD system provided the best performance

Availability of Japanese Government's supplemental texts on radiation reflecting the Fukushima Daiichi Nuclear Power Plant accident for elementary and secondary education from dental students' understanding

Following the Fukushima Nuclear Power Plant accident, the Japanese government created two supplemental texts about radiation reflecting the accident for elementary, middle school, and high school students. These texts were made to explain radiation and consequently to obtain public consent for the continuation of the nuclear program. The present study aimed to evaluate the appropriateness of the content of the texts and to collect the basic data on the level of understanding necessary to improve radiation education. Lectures on radiology including nuclear energy and the Fukushima accident were given to 44 fourth-year dental students in 2013. The questionnaire was administered in 2014 when these students were in their sixth-year. The survey was also administered to 40 first-year students and 41 fourth-year students who hadn't any radiology lectures. Students rated their level of understanding of 50 phrases used in the texts on a four-point scale (understanding = 3, a little knowledge = 2, having heard = 1, no knowledge = 0). Questions on taking an advanced physics course in high school and means of learning about radiation in daily life were also asked. The level of understanding of phrases in the supplemental text for middle and high school students was significantly higher among sixth-year students (mean = 1.43) than among first-year (mean = 1.12) or fourth-year (mean = 0.93) students (p < 0.05). Overall, the level of understanding was low, with scores indicating that most students knew only a little. First-year students learning about radiation from television but four-year and six-year students learning about radiation from newspaper scored significantly higher (p < 0.05). It was concluded that radiation education should be improved by using visual material and preparing educators to teach the material for improving the public's understanding of radiation use—especially nuclear power generation because the phrases used in the supplementary texts are very difficult for students to understand

Genomic Epidemiology of Multidrug-Resistant Mycobacterium tuberculosis During Transcontinental Spread

The transcontinental spread of multidrug-resistant (MDR) tuberculosis is poorly characterized in molecular epidemiologic studies. We used genomic sequencing to understand the establishment and dispersion of MDR Mycobacterium tuberculosis within a group of immigrants to the United States. We used a genomic epidemiology approach to study a genotypically matched (by spoligotype, IS6110 restriction fragment length polymorphism, and mycobacterial interspersed repetitive units-variable number of tandem repeat signature) lineage 2/Beijing MDR strain implicated in an outbreak of tuberculosis among refugees in Thailand and consecutive cases within California. All 46 MDR M. tuberculosis genomes from both Thailand and California were highly related, with a median difference of 10 single-nucleotide polymorphisms (SNPs). The Wat Tham Krabok (WTK) strain is a new sequence type distinguished from all known Beijing strains by 55 SNPs and a genomic deletion (Rv1267c) associated with increased fitness. Sequence data revealed a highly prevalent MDR strain that included several closely related but distinct allelic variants within Thailand, rather than the occurrence of a single outbreak. In California, sequencing data supported multiple independent introductions of WTK with subsequent transmission and reactivation within the state, as well as a potential super spreader with a prolonged infectious period. Twenty-seven drug resistance-conferring mutations and 4 putative compensatory mutations were found within WTK strains. Genomic sequencing has substantial epidemiologic value in both low- and high-burden settings in understanding transmission chains of highly prevalent MDR strain

Tuberculosis-Related Deaths within a Well-Functioning DOTS Control Program

To describe the molecular epidemiology of tuberculosis (TB)-related deaths in a well-managed program in a low-HIV area, we analyzed data from a cohort of 454 pulmonary TB patients recruited between March 1995 and October 2000 in southern Mexico. Patients who were sputum acid-fast bacillus smear positive underwent clinical and mycobacteriologic evaluation (isolation, identification, drug-susceptibility testing, and IS6110-based genotyping and spoligotyping) and received treatment from the local directly observed treatment strategy (DOTS) program. After an average of 2.3 years of follow-up, death was higher for clustered cases (28.6 vs. 7%, p=0.01). Cox analysis revealed that TB-related mortality hazard ratios included treatment default (8.9), multidrug resistance (5.7), recently transmitted TB (4.1), weight loss (3.9), and having less than 6 years of formal education (2). In this community, TB is associated with high mortality rates