Repeated supra-maximal sprint cycling with and without sodium bicarbonate supplementation induces endothelial microparticle release

Under normal homeostatic conditions, the endothelium releases microparticles (MP), which are known to increase under stressful conditions and in disease states. CD105 (endoglin) and CD106 (vascular cell adhesion molecule-1) are expressed on the surface of endothelial cells and increased expression in response to stress may be observed. A randomised-controlled double-blinded study aimed to examine the use of endothelial microparticles as a marker for the state of one’s endothelium, as well as whether maintaining acid-base homeostasis affects the release of these MP. This study tested seven healthy male volunteers, who completed a strenuous cycling protocol, with venous blood analysed for CD105+ and CD106+ MP by flow cytometry at regular intervals. Prior to each trial participants consumed either 0.3 g·kg-1 body mass of sodium bicarbonate (NaHCO3), or 0.045 g·kg-1 body mass of sodium chloride (NaCl). A significant rise in endothelial CD105+MP and CD106+MP (p < 0.05) was observed at 90 minutes post exercise. A significant trend was shown for these MP to return to resting levels 180 minutes post exercise in both groups. No significance was found between experimental groups, suggesting that maintaining acid-base variables closer to basal levels has little effect upon the endothelial stress response for this particular exercise mode. In conclusion, strenuous exercise is accompanied by MP release and the endothelium is able to rapidly recover in healthy individuals, whilst maintaining acid-base homeostasis does not attenuate the MP release from the endothelium after exercise

Implications of a pre-exercise alkalosis-mediated attenuation of HSP72 on its response to a subsequent bout of exercise

The aim of this study was to investigate if a pre-exercise alkalosis-mediated attenuation of HSP72 had any effect on the response of the same stress protein after a subsequent exercise. Seven physically active males [25.0 ± 6.5 years, 182.1 ± 6.0 cm, 74.0 ± 8.3 kg, peak aerobic power (PPO) 316 ± 46 W] performed a repeated sprint exercise (EXB1) following a dose of 0.3 g kg⁻¹ body mass of sodium bicarbonate (BICARB), or a placebo of 0.045 g kg⁻¹ body mass of sodium chloride (PLAC). Participants then completed a 90-min intermittent cycling protocol (EXB2). Monocyte expressed HSP72 was significantly attenuated after EXB1 in BICARB compared to PLAC, however, there was no difference in the HSP72 response to the subsequent EXB2 between conditions. Furthermore there was no difference between conditions for measures of oxidative stress (protein carbonyl and HSP32). These findings confirm the sensitivity of the HSP72 response to exercise-induced changes in acid–base status in vivo, but suggest that the attenuated response has little effect upon subsequent stress in the same day

Heat Analysis Manager (HAM), a Thermal Desktop API Based Heat Map Generation Software

Thermal engineers often create custom heat maps to analyze their thermal model. However, generating a heat map is difficult because thermal simulation only readily provide attributes of simulated nodes such as temperature, capacitance, heat generation, and a network of conductances. Heat flow values are a derived quantity from the nodal attributes, and the data processing and management of heat flow between nodes quickly become difficult for large models. Deriving a network of heat flow values requires vast amount of calculations and data handling, heat map generation process generally suffers from processing speed, loss of accuracy, and/or presentation of data in a useful format. Heat Analysis Manager (HAM) is a Thermal Desktop (TD) based free multi-purpose tool developed to aid thermal engineers in analyzing their thermal model, including a heat map generation functionality. HAMs heat map generator retains accuracy and fast processing speed by utilizing TDs application programming interface (API) and built-in TDs Qflow from Results. Furthermore, HAMs heat map output is presented in an easily customizable format in Excel, allowing users to create various custom visual heat maps. A full description of how HAM utilizes TDs API to create a customizable heat map is provided. A simple model demonstration is included along with step-by-step procedures on creating custom heat maps. HAMs heat map result has been verified against TDs and other heat map generation software, and verification methods are also included

Implications of a Pre-Exercise Alkalosis Mediated Attenuation of HSP72 on its Response to a Subsequent Bout of Exercise

The aim of this study was to investigate if a pre-exercise alkalosis mediated attenuation of HSP72 had any effect on the response of the same stress protein after a subsequent exercise. Seven physically active males (25.0 ± 6.5 years, 182.1 ± 6.0 cm, 74.0 ± 8.3 kg, peak aerobic power (PPO) 316 ± 46 W) performed a repeated sprint exercise (EXB1) following a dose of 0.3 g kg-1 body mass of sodium bicarbonate (BICARB), or a placebo of 0.045 g kg-1 body mass of sodium chloride (PLAC). Participants then completed a 90-min intermittent cycling protocol (EXB2). Monocyte expressed HSP72 was significantly attenuated after EXB1 in BICARB compared to PLAC, however there was no difference in the HSP72 response to the subsequent EXB2 between conditions. Furthermore there was no difference between conditions for measures of oxidative stress (protein carbonyl and HSP32). These findings confirm the sensitivity of the HSP72 response to exercise induced changes in acid-base status in vivo, but suggest that the attenuated response has little effect upon subsequent stress in the same day

Autonomous Robot Sphere

The Autonomous Robot Sphere is an interactive robot toy meant to entertain kids. The robot will locate its target and execute algorithms to autonomously evade or chase a child. The sphere will contain a platform equipped with four omni-wheels, which will allow the sphere to maneuver and change direction almost instantaneously. The robot will be configured to maintain a fixed distance from the transmitter, allowing it to chase or evade the child in response to their movement. The primary advantage of our design lies in its capability to quickly adapt to changes in direction

Improved conceptual generation and selection with transcranial direct current stimulation in older adults

Normal aging is associated with deficits in various aspects of spoken language production, including idea generation and selection, and involves activity in frontal brain areas including left inferior frontal cortex (LIFG). These conceptual preparation processes, largely involving executive control, precede formulation and articulation stages and are critical for language production. Noninvasive brain stimulation (e.g., transcranial direct current stimulation, tDCS) has proven beneficial for age-related fluency and naming deficits, but this has not been extended to conceptual preparation mechanisms.We investigated whether tDCS could facilitate idea generation and selection in 24 older adults aged 60-80\ua0years. In the first phase, participants completed an idea generation test and a selection test with no stimulation. In the second phase they completed an alternate version of the tests in conjunction with either active or sham stimulation. Active stimulation applied 1-mA anodal tDCS over LIFG for the test duration (10\ua0min).\ua0 Responses were faster following active stimulation than following sham. Furthermore, improvements were specific to test conditions involving novel generation (p\ua0=\ua0.030) and selection (p\ua0=\ua0.001) and were not observed in control conditions for which these mechanisms were minimally involved.\ua0 We concluded that tDCS benefits conceptual preparation mechanisms. This preliminary evidence is an important step for addressing age-related decline in propositional language generation, which is integral to conversational speech. This approach could also be extended toward rehabilitation in neurological patients with deficits in these processes

Leading Edge Boundary Layer Suction Device for the Cal Poly Rolling Road Wind Tunnel

Over the course of three quarters from Fall of 2016 to Spring of 2017, our team designed and built a boundary layer suction device. The boundary layer suction device has three main functions: a scoop that redirects most of the boundary layer air out of the wind tunnel, fans that suck the remaining boundary layer air through a porous plate and ducting and out of the wind tunnel, and a transition bridge that transitions the remaining air smoothly onto the rolling road. The wind tunnel is owned by Cal Poly and the rolling road is a new addition to it. By the end of our project, the rolling road was not yet functional. A variable-frequency drive (VFD) will be installed over the summer and belt suction fans will also be installed. Once these are in place, the rolling road can be used. We were successful in building our device and installing it, but once the rolling road is functional, further iterations can be made on our device. The framing and ducting will likely stay in place without further iterations. However, which fans are used can be changed around. We designed our device with an American Fan model AF-10 in mind, but this fan cannot be used until the VFD is installed. There are other fans that can be repurposed and tested on this device as well, though. And, if necessary, a more powerful fan could be purchased. The lid, consisting of the scoop, porous plate, and transition bridge, was also designed to be flexible enough for further iterations. All three of its components are separate pieces that fasten to each other and the lid itself is separate from the rest of the assembly and is only meant to attach to it during tests. Two issues could crop up with the scoop: less air than expected being redirected through the scoop, and the scoop creating flow separation. If the latter issue occurs, a new scoop could be made with the angle (currently 10 degrees) reduced. If the former issue occurs, a new scoop could be made with longer overhang, or ducting could be made from where the flow is redirected, to the end of the tunnel. The following report details the process we went through to make this device. It provides details on the design process, final design analysis, manufacturing results, and test plans that show our progress from project ideation all the way to design acceptance and verification