Impacts of a Novel Non-Stimulant-Based Ergogenic Supplement Blend on Maximal Oxygen Uptake and Time to Fatigue: A Pilot Study

CrossFit athletes are frequently subjected to high-intensity training sessions that demand commensurately robust muscular and cardiovascular endurance capacities. Despite the propensity of research investigating the role of aerobically-associated ergogenic aids, few have elucidated their combination in highly trained athletic populations. PURPOSE: to evaluate the preliminary efficacy of a novel supplement comprising several empirically-supported ingredients and their potential ability to impact the aerobic performance in experienced CrossFit athletes. METHODS: Six experienced CrossFit athletes (3M/3F; aged 41±13y; training 5±1 times/week) ingested 2.0mL daily of a novel supplement proprietary formulation (Flow, Cerus, Vero Bleach, FL) containing Beetroot (Beta Vulgaris), Ashwagandha (Withania Somnifera), Arjuna (Terminalia Arjuna), Rhodiola (Rhodiola Rosea), as well as Cayenne (Capsicum Annuum) for 28 days between a baseline and post-supplementation maximal oxygen consumption (VO2MAX) assessment. Participants were asked not to change their exercise habits and consumed the aforementioned supplement 30 minutes prior to each training session and/or during the morning on rest days. A 4.0mL supplement dose was additionally administered 30 minutes prior to the post-supplementation aerobic capacity assessment. Both the treadmill VO2MAX and test time-to-fatigue (elapsed finish-start time) were analyzed using separate paired-samples t-tests at a significance level of pRESULTS: Although VO2MAX did not significantly increase (p=.146; Hedge’s G=.737), the participants’ treadmill assessment TTE demonstrated a statistically significant improvement (p=.012, Hedge’s G=.726) from pre- to post-supplementation (10.67 to 12.11 minutes [13.55%], respectively). Notably, the 2/6 participants that experienced pre- to post-supplementation VO2MAX decrements tested positive for COVID-19 during their supplementation period. CONCLUSION: The present pilot study showcases the potential of a novel aerobically-associated ergogenic supplement. Nevertheless, future research should more rigorously evaluate these data by employing a randomized, double-blinded, and placebo-controlled design, as well as a larger sample size before any definitive conclusions may be inferred

Acute Maltodextrin Supplementation During Resistance Exercise

PURPOSE: Most of the research investigating the ergogenic enhancing mechanisms of carbohydrate have been conducted using aerobic based exercise. Therefore, the purpose of this study was to investigate the effects of pre-exercise maltodextrin ingestion on resistance exercise performance, serum insulin, epinephrine, glucose, and muscle glycogen concentrations. METHODS: In a double blind, cross over, repeated measures design, participants completed four sets to failure at 70% of 1-RM with 45s rest on the angled leg press with or without pre-exercise maltodextrin (2g/kg) after a 3hr fast. Serum glucose, epinephrine, and insulin were assessed at baseline, 30 min post-ingestion, immediately after, and 1hr post-exercise with or without carbohydrate supplementation. Muscle glycogen was assessed from biopsy specimens sampled from the vastus lateralis before supplementation, immediately after exercise, and 1hr post exercise under both conditions. RESULTS: There was no main effect of supplement on resistance exercise performance (p=.18). Muscle glycogen concentration decreased across time for both groups (p\u3c.001). There was an interaction in serum glucose decreasing more during exercise in the carbohydrate condition (p=.026). An interaction occurred showing insulin decreased during exercise in the carbohydrate condition (p=.003). Also, there was a main effect of insulin being elevated with carbohydrate consumption (p=.027). Epinephrine was decreased across all time points after carbohydrate ingestion (p=.023). CONCLUSION: Carbohydrate supplementation before resistance exercise did not improve leg press performance to fatigue despite increased metabolic substrate availability. These results indicate that pre-exercise dietary carbohydrate will be utilized preferentially during exercise due to decreased epinephrine, decreased serum glucose, and increased insulin concentrations. However, the increases in glycolytic substrate availability will not increase exercise performance or glycogen content following 1hr of recovery

Fiber Type Profile and its Relation to Wilks Coefficient in Both Male and Female Powerlifters

While powerlifters tend to display higher fast-twitch fiber content, it is unknown if this content predicts competitive performance via Wilks coefficient. Purpose: to 1) compare the myosin heavy chain (MHC) fiber type (FT) profiles between powerlifters and sedentary controls of both sexes, and 2) determine if fast-twitch fiber content predicts Wilks coefficient. Methods: Twelve actively competing powerlifters (PL; n=6M/6F; age=21±1.0y; 3.0±1.8y competing; 7.3±6.6 meets attended) and ten sedentary controls (CON; n=5M/5F; age=19.4±2.0y) underwent vastus lateralis muscle biopsies, with samples analyzed for MHC isoform content via mixed homogenate SDS-PAGE. Individual MHC isoform differences between group and sex were analyzed using a 3x2x2 (FT [MHC I, IIa, & IIx] x group [PL & CON] x sex [male & female]) ANOVA and MHC IIa content was compared to Wilks coefficient using Pearson correlation coefficient at pResults: Male PL MHC isoform distribution was 50±6% I, 45±6% IIa, and 5±11% IIx, vs 46±6% I, 53±6 IIa, and 0% IIx in PL females. Conversely, male CON MHC distribution was 33±5% I, 38±7% IIa, and 30±8% IIx, vs 35±9% I, 44±8% IIa, and 21±17% IIx in CON females. Analysis revealed a significant FT main effect (pConclusions:These results illustrate powerlifters have higher MHC I and IIa proportions, as well as lower MHC IIx content compared to sedentary controls. While overall limited by sample size, MHC IIa content does not appear to be a significant predictor of powerlifting Wilks coefficient, suggesting this characteristic alone does not define powerlifter skill variations

Influence of Acute Turkesterone Dosing on Resting Metabolic Rate and Substrate Utilization in Recreationally-active Males

Turkesterone is a relatively novel phytoecdysteroid compound that has become increasingly popular amongst recreationally active adults seeking to improve body composition. Although many of the these hypothetical benefits arose from prior rodent data demonstrating enhanced substrate utilization, no data presently exist amongst humans in this regard. PURPOSE: to determine the effect of multiple turkesterone doses on both resting metabolic rate (RMR) and substrate utilization in a healthy human population. METHODS: Eleven recreationally active males (23.3±2.2y) visited the laboratory on three occasions separated by at least seven days and were randomized in single-blind, placebo-controlled, and counter-balanced crossover fashion to either 2000mg cellulose placebo (PLA), 1000mg turkesterone + 1000mg placebo, (1000T) or 2000mg (2000T) turkesterone. RMR and respiratory exchange ratio were assessed using a metabolic cart for 20 minutes prior to supplement provision (i.e. baseline [PRE)), as well as 60-minutes (POST60M), 120-minutes (POST120M), and 180-minutes (POST180M) post-acute supplementation timepoints at each visit. RMR, as well as both carbohydrate (CHO) and Fat (FAT) oxidation were analyzed using a two-way (condition [PLA, 1000T, 2000T] x time [PRE, POST60M, POST120M, POST180M) ANOVA with repeated measures at a significance level of pRESULTS: Analyses failed to reveal any significant condition, time, nor interaction effects for RMR, nor CHO or FAT oxidation (p\u3e0.05). Nonetheless, both 1000T (2.7%, 5.6%, and 7.8%) and 2000T (0.7%, 4.2%, and 3.6%) increased mean RMR above baseline at POST60M, POST120M, and POST180M timepoints, respectively. Conversely, PLA decreased mean RMR by 0.9% and 0.7% at POST60M and POST120M, respectively. Incidentally, the 1000T condition displayed increased mean FAT oxidation by 1.85, 5.34, and 7.96% at the POST60M, POST120M, and POST180M timepoints, respectively, and when compared to the consistent decreases observed with both PLA and 2000T. CONCLUSION: Although these data fail to display a significant turkesterone-mediated enhancement in the investigated metabolic parameters, there were interesting mean differences that should be further explored to determine any longitudinal and/or exercise-dependent permissive impacts on RMR and substrate utilization

Creatine Supplementation Improves Performance Without Deleterious Cardiovascular Parameters in Trained Females

Creatine monohydrate supplementation is broadly substantiated to elicit both favorable ergogenic and health-associated advantages amidst vast demographics. Nevertheless, its impacts on human cardiovascular outcomes are hitherto undescribed in generally healthy populations. Furthermore, females are largely underrepresented in the ergogenic creatine literature and their potentially differential cardiovascular responses are unknown. PURPOSE: to determine whether young, healthy females would glean discernible hypertrophy and enhanced performance, commensurate to favorable post-resistance training cardiovascular parameters and relative to placebo-matched controls across a four week training timeline. METHODS: Twenty-eight resistance trained women (25.5±6.1 years) were randomly assigned to supplement creatine monohydrate (CRE; 5g creatine monohydrate + 5g dextrose) or placebo (PLA; 10g dextrose) four times per day for 7 days in double-blind fashion. Each subject subsequently completed three resistance training sessions per week for four weeks that involved four consecutive sets to muscular failure of both smith machine half-squat (SMHS) and leg press exercise. The change in body mass, exercise repetition number, rated perceived exertion (RPE), and cardiovascular variables (heart rate, as well as systolic and diastolic blood pressures) were assessed at sessions 1, 6, and 12. All aforementioned experimental dependent variables were assessed with multiple three-way (supplement [CRE, PLA] x session [1, 6, 12] x set [1st, 2nd, 3rd]) mixed model ANOVA with repeated measures at a significance level of p≤0.05. RESULTS: Analyses revealed a significant CRE-specific body mass increase (p=0.013; 57.9±7.7kg to 58.7±7.7kg), as well as significant SMHS (p=0.006) and leg press (p=0.017) repetitions per set versus PLA. Additionally, CRE demonstrated significantly lower relative RPE values at session 12 compared with sessions 1 and 6. Analyses failed to reveal any significant main or interaction effects for any aforementioned cardiovascular variable. CONCLUSION: The present data both demonstrate the safety of combined creatine monohydrate supplementation and resistance training on cardiovascular parameters, whilst simultaneously substantiating its ergogenic efficacy in females subjects

A Cross-Sectional Examination of Wrist Wrap Use Prevalence and Characterization for Ergogenic Purposes in Actively Competing Powerlifters

Purpose: Wrist wraps are regularly incorporated by competitive powerlifters, but several product-specific variations may influence any potential ergogenic benefits. In addition, the prevalence of athletic wrist wrap use is hitherto undescribed. Methods: Seventy actively competing powerlifters (n=70; 27±6y) who competed in the last two years were randomly recruited at sanctioned meets, whereby wrist wrap use descriptive data (wrap style [F or S], wrap length, and events used), wrap tightness (assessed via pulse oximeter-detected oxygen saturation (SpO2) and subjective discomfort [Borg CR10+]), as well as post-meet bench press one repetition maximum (1RM) were collected. Wrist wrap use prevalence data (wrap style, wrap length, and events used) were compared across TX, NC/SC, and CA regions, along with any potential correlations between both region-collapsed wrapped SpO2 and bench press. Finally, predictors of bench press 1RM (weight [kg], age [y], stiff wrap, wrap length, wrapped discomfort [WCR10+], and WSpO2) were assessed using linear regression, whereby all aforementioned statistical analyses were set at a significance level of p\u3c.05. Results: Analyses failed to detect any significant regional differences in wrap style, length, or events used (p\u3e0.05). Moreover, linear regression analysis revealed a significant effect (r2= 0.851, p = 0.02) where weight solely predicted bench press 1RM (p = 0.0433). Conclusions: Although we failed to detect any significant wrist wrap relationships, actively competing powerlifters nonetheless prominently utilize wraps across the regions assessed. Therefore, the potential for wrist wraps to augment bench press performance warrants further elucidation in a controlled, standardized investigation

Ubiquitin Proteasome System Activity is Suppressed by Curcumin Following Exercise-Induced Muscle Damage in Human Skeletal Muscle

PURPOSE: Curcumin is a natural polyphenolic compound with antioxidant and anti-inflammatory properties. In vitro and in vivo animal studies have demonstrated that exposure to curcumin leads to dysregulation of the ubiquitin-proteasome system (UPS). However, to date, no study has investigated curcumin’s ability to influence UPS activity in a human model. Therefore, the purpose of this study was to investigate the effects of curcumin supplementation on markers of UPS activity in the presence of elevations in UPS activity due to exercise-induced muscle damage. METHODS: Twenty-three recreationally active male and female participants between the ages of 18-30 were randomized into a curcumin (CUR; n=11) or placebo (PLA; n=12) group. Both groups were instructed to consume 2 g of their respective supplement and 20 mg of piperine for 11 consecutive days. Following 8 consecutive days of supplementation, participants performed a 45 minute eccentrically-biased muscle damaging treadmill protocol at 60% VO2max. Muscle biopsies and delayed onset muscle soreness (DOMS) analyses were performed 30 minutes prior and 3, 24, 48, and 72 hours following exercise-induced muscle damage. Skeletal muscle ubiquitin, MAFbx/Atrogin-1, ubiquitin specific peptidase 19 (USP19), and chymotrypsin-like protease concentrations were measured using ELISA. A 3-way repeated measures ANOVA with pairwise comparisons was conducted with significance set at p≤0.05. RESULTS: Both groups had a significant time effect for DOMS (p \u3c .001). Pairwise comparisons indicated DOMS was significantly greater from baseline at all time points except 72 hours following muscle damage. No significant differences were found for USP19 between groups. Regardless of time, a significant main effect for condition was observed for ubiquitin (p=.016) and MAFbx/Atrogin-1 (p=.006) where CUR was significantly lower than PLA. Additionally, a significant main effect for gender was observed for MAFbx/Atrogin-1 (p=.013) where females were greater than males. A significant group x gender interaction was found for chymotrypsin-like protease (p = .049) where males had lower values with curcumin supplementation while females had slightly higher. CONCLUSION: Curcumin supplementation in humans does appear to dysregulate UPS activity in the presence of exercise-induced muscle damage. Specifically, curcumin’s ability to suppress protein ubiquination provides preliminary evidence of curcumin supplementation’s potential therapeutic role in decreasing protein degradation associated with skeletal muscle damage and perhaps other atrophic scenarios

Wnt/β-Catenin and Androgen Receptor Signaling Increase Following High Load Resistance Exercise Without Elevations in Serum/Muscle Testosterone or Androgen Receptor Content

PURPOSE: The purpose of this study was 1) to determine the effect of single bouts of volume- and intensity-equated low (LL) and high load (HL) full-body resistance exercise (RE) on AR-DNA binding, serum/muscle testosterone and dihydrotestosterone, muscle androgen receptor (AR), and AR-DNA binding and 2) to determine the effect of RE on sarcoplasmic and nucleoplasmic β-catenin concentrations in order to determine their impact on mediating AR-DNA binding in the absence/presence of serum/muscle androgen and AR protein. METHODS: In a cross-over design, ten resistance-trained males completed volume- and intensity-equated LL and HL full-body RE. Blood and muscle samples were collected at pre-, 3h-, and 24h post-exercise. Separate 2x3 factorial ANOVAs with repeated measures and pairwise comparisons with a Bonferroni adjustment were used to analyze main effects. RESULTS: No significant differences were observed in muscle AR, testosterone, dihydrotestosterone, or serum total testosterone in either condition (p \u3e.05). Serum free testosterone was significantly decreased 3h post-exercise and remained significantly less than baseline 24h post-exercise in both conditions (p.05). Moreover, sarcoplasmic β-catenin was significantly greater in HL (p.05). CONCLUSION: Increases in AR-DNA binding in response to HL indicates AR signaling may be load-dependent. Furthermore, despite the lack of increase in serum and muscle androgens or AR content following HL RE, elevations in AR-DNA binding with elevated sarcoplasmic β-catenin suggests β-catenin may be facilitating this response

Combined Blood Flow Restriction Training and Betaine Supplementation Impacts on Serum Betaine and Homocysteine Concentrations

Homocysteine (HCY) is a clinically implicated in inflammation and cardiovascular impairments. Although both betaine supplementation and acute resistance (both high-load [HL] and low-load blood flow restriction [LL-BFR]) training notably attenuate HCY concentrations, it is hitherto unknown if these independent modalities synergistically interact. PURPOSE: to determine whether a combination of betaine supplementation, as well as acute HL and/or LL-BFR training can attenuate post-exercise HCY more effectively than either isolated modality. METHODS: Eighteen recreationally trained males (25±5y) were randomized in double-blind fashion to supplement 6g/day of either betaine anhydrous (BET) or identically dosed cellulose placebo for 14-days. Subsequently, all subjects performed four standardized sets of one-leg press and two additional sets to muscular failure on both legs in a counter-balanced and crossover design. Specifically, one leg performed standard high-load (HL; 70%1RM) exercise and contralateral limb underwent BFR (LL-BFR; 20%1RM) training at 80% arterial occlusion pressure. Serum homocysteine (HCY) and betaine (BET) concentrations were analyzed before and 30-minutes post-exercise prior to quantification via ELISA and liquid chromatography-mass spectrometry, respectively. The changes in all aforementioned variables from baseline (∆HCY and ∆BET) were assessed via separate two-way mixed model ANOVA with repeated measures at a significance level of pRESULTS: Analyses failed to reveal any significant main nor interaction effects for serum ∆BET. Although no apparent main supplement nor interaction effects were observed, ∆HCY demonstrated a significant main exercise condition effect (p=.045; ηp2=.228), whereby the LL-BFR group displayed significantly greater concentrations versus HL (p=.045). CONCLUSION: While these findings ultimately do not support a betaine-resistance training synergy-mediated reduction in serum HCY, our data otherwise suggest BFR training may preferentially result in lower post-training concentrations relative to a commonly employed, high-load approach. Future research should elucidate the credence of this interpretation via additional longitudinal investigations amidst hyperhomocysteinemia-predisposed clinical populations

Influence of Acute Turkesterone Dosing on Serum Insulin-like Growth Factor 1 (IGF-1) and Subjective Digestibility Scores in Recreationally-active Males

Turkesterone is a relatively novel phytoecdysteroid compound that has become increasingly popular amongst recreationally active demographics. Despite prior in vitro data suggesting that this compound may support enhanced body composition via both insulin-like growth factor 1 (IGF-1)-mediated protein synthesis, no human evidence exists in this regard nor how well its digestibility is tolerated. PURPOSE: To determine the effect of multiple turkesterone doses on serum IGF-1 and to report any gastrointestinal (GI) distress symptoms in a healthy human sample. METHODS: Eleven recreationally active males (23.3±2.2y) visited the laboratory on three occasions separated by at least seven days and were randomized in single-blind, placebo-controlled, and counter-balanced crossover fashion to either 2000mg cellulose placebo (PLA), 1000mg turkesterone + 1000mg placebo, (1000T) or 2000mg (2000T) turkesterone. Venous blood was sampled to determine serum IGF-1 concentrations and a GI distress questionnaire was (nausea, vomiting, heartburn symptoms, etc.) administered both at baseline (PRE), as well as 3-hours (POST3H) and 24-hours (POST24H) post-acute supplementation at each visit. Serum IGF-1 was analyzed using a two-way (condition [PLA, 1000T, 2000T] x time [PRE, POST3H, POST24H]) ANOVA with repeated measures at a significance level of pRESULTS: Analyses failed to reveal any significant condition (p=.180; ηp2=0.228), time (p=0.227; ηp2=.390), nor interaction effects (p=0.547; ηp2=0.211) for serum IGF-1. Moreover, no participants reported any GI distress symptoms across any condition and/or time permutation. CONCLUSION: Although the current study did not find any significant IGF-1-associated serum alterations to multiple acute turkesterone doses in the times assessed, there were fortunately no adverse GI symptoms experienced by the participants across any dose throughout the investigation. Nevertheless, these data support turkesterone supplementation is well tolerated and thus future research should build upon our analysis by employing a longitudinal supplementation regimen alongside an exercise intervention to elucidate the potential long-term and anabolism-permissive impacts of this compound on the presently-explored and additional associated parameters