Hydrophilic Interaction Liquid Chromatography-Electrospray Ionization Mass Spectrometry for Therapeutic Drug Monitoring of Metformin and Rosuvastatin in Human Plasma

In this work a hydrophilic interaction liquid chromatography/positive ion electrospray mass spectrometric assay (HILIC/ESI-MS) has been developed and fully validated for the quantitation of metformin and rosuvastatin in human plasma. Sample preparation involved the use of 100 µL of human plasma, following protein precipitation and filtration. Metformin, rosuvastatin and 4-[2-(propylamino) ethyl] indoline 2 one hydrochloride (internal standard) were separated by using an X-Bridge-HILIC BEH analytical column (150.0 × 2.1 mm i.d., particle size 3.5 µm) with isocratic elution. A mobile phase consisting of 12% (v/v) 15 mM ammonium formate water solution in acetonitrile was used for the separation and pumped at a flow rate of 0.25 mL min−1 . The linear range of the assay was 100 to 5000 ng mL−1 and 2 to 100 ng mL−1 for metformin and rosuvastatin, respectively. The current HILIC-ESI/MS method allows for the accurate and precise quantitation of metformin and rosuvastatin in human plasma with a simple sample preparation and a short a chromatographic run time (less than 15 min). Plasma samples from eight patients were further analysed proving the capability of the proposed method to support a wide range of clinical studies

EuroDRONE, A European unmanned traffic management testbed for U-space

EuroDRONE is an Unmanned Traffic Management (UTM) demonstration project, funded by the EU’s SESAR organization, and its aim is to test and validate key UTM technologies for Europe’s ‘U-Space’ UTM program. The EuroDRONE UTM architecture comprises cloud software (DroNav) and hardware (transponder) to be installed on drones. The proposed EuroDRONE system is a Highly Automated Air Traffic Management System for small UAVs operating at low altitudes. It is a sophisticated, self-learning system based on software and hardware elements, operating in a distributed computing environment, offering multiple levels of redundancy, fail-safe algorithms for conflict prevention/resolution and assets management. EuroDRONE focuses its work on functionalities which involve the use of new communication links, the use of vehicle-to-infrastructure (V2I) and vehicle-to-vehicle (V2V) technology to communicate information between drones and operators for safe and effective UTM functionality. Practical demonstrations that took place in Patras/Messolonghi in 2019 are presented and show the benefits and shortcomings of near-term UTM implementation in Europe

EuroDRONE, a European Unmanned Traffic Management Testbed for U-Space

EuroDRONE is an Unmanned Traffic Management (UTM) demonstration project, funded by the EU's SESAR organization, and its aim is to test and validate key UTM technologies for Europe's `U-Space' UTM program. The EuroDRONE UTM architecture comprises cloud software (DroNav) and hardware (transponder) to be installed on drones. The proposed EuroDRONE system is a Highly Automated Air Traffic Management System for small UAVs operating at low altitudes. It is a sophisticated, self-learning system based on software and hardware elements, operating in a distributed computing environment, offering multiple levels of redundancy, fail-safe algorithms for conflict prevention/resolution and assets management. EuroDRONE focuses its work on functionalities which involve the use of new communication links, the use of vehicle-to-infrastructure (V2I) and vehicle-to-vehicle (V2V) technology to communicate information between drones and operators for safe and effective UTM functionality. Practical demonstrations that took place in Patras/Messolonghi in 2019 are presented and show the benefits and shortcomings of near-term UTM implementation in Europe