Transcriptomics datasets for Clostridium autoethanogenum grown on CO in different environmental conditions

The datasets contain comparative transcriptome data of Clostridium autoethanogenum in different environmental conditions, as described in Chapter 6 of the PhD Thesis of Martijn Diender (February 2019). Data was collected and analysed at Wageningen University, the laboratory of microbiology and is part of a project funded by a SIAM gravitation grant (024.002.002

Genome assembly of a novel psychrotolerance bacterium, Trichococcus ART1 .

A psychrotolerant anaerobe, strain ART1T, was isolated from a psychrophilic anaerobic digester treating . 16S rRNA gene sequence of strain ART1T was highly similar to those of other Trichococcus species (> 99%), but digital DNA-DNA hybridization (dDDH) values were lower than 70% indicating that strain ART1 is a new species of the genus Trichococcus. Cells of strain ART1T were immotile cocci and stained Gram-positive. Growth was optimal at pH 7.5 and cells could grow in a temperature range of 0 to 37°C (optimum 30°C). Strain ART1T could degrade several carbohydrates, and the main products from glucose fermentation are lactate, acetate, formate, and ethanol.

Genome assembly of a novel psychrotolerance bacterium, Trichococcus ART1 .

A psychrotolerant anaerobe, strain ART1T, was isolated from a psychrophilic anaerobic digester treating . 16S rRNA gene sequence of strain ART1T was highly similar to those of other Trichococcus species (> 99%), but digital DNA-DNA hybridization (dDDH) values were lower than 70% indicating that strain ART1 is a new species of the genus Trichococcus. Cells of strain ART1T were immotile cocci and stained Gram-positive. Growth was optimal at pH 7.5 and cells could grow in a temperature range of 0 to 37°C (optimum 30°C). Strain ART1T could degrade several carbohydrates, and the main products from glucose fermentation are lactate, acetate, formate, and ethanol.

Genome assembly of a novel psychrotolerance bacterium, Trichococcus ART1 .

A psychrotolerant anaerobe, strain ART1T, was isolated from a psychrophilic anaerobic digester treating . 16S rRNA gene sequence of strain ART1T was highly similar to those of other Trichococcus species (> 99%), but digital DNA-DNA hybridization (dDDH) values were lower than 70% indicating that strain ART1 is a new species of the genus Trichococcus. Cells of strain ART1T were immotile cocci and stained Gram-positive. Growth was optimal at pH 7.5 and cells could grow in a temperature range of 0 to 37°C (optimum 30°C). Strain ART1T could degrade several carbohydrates, and the main products from glucose fermentation are lactate, acetate, formate, and ethanol.

Comparative genomic and physiological analysis of Trichococcus species

Axenic cultures of Trichococcus species will be analysed, with a special emphasis on Trichococcus strain ES5 that can produce PDO from glycerol. PDO can be used to compose polyesters and polyurethanes or it can be used as solvent and an antifreeze/protective agent5,6; its production from glycerol is attractive because this is a low cost substrate (byproduct resulting from the production of biodiesel). Among the known Trichoccocus species, production of PDO from glycerol was only observed by Trichococcus strain ES5. Although Trichococcus strains share high similarity in their 16S RNA gene, differences at genomic level seem to be much higher and low DNA-DNA hybridization values were observed for the different species7. We have currently a collaboration with the German Collection of Microorganisms (DSMZ) and the Joint Genome Institute (JGI) for sequencing the genomes of all the thus far isolated Tricochoccus species, and the comparative analysis of these genomes will be done within this PhD project. Further on, proteomics will be performed to study specific physiological properties. For example, one fascinating physiological characteristic of Trichococcus species is the capability of growing at very low temperatures – specifically, Trichococcus patagoniensis8 can be a targeted species to study this feature as it can grow at -5oC by producing a mucous substance that covers the cells

Comparative genomic and physiological analysis of Trichococcus species

Axenic cultures of Trichococcus species will be analysed, with a special emphasis on Trichococcus strain ES5 that can produce PDO from glycerol. PDO can be used to compose polyesters and polyurethanes or it can be used as solvent and an antifreeze/protective agent5,6; its production from glycerol is attractive because this is a low cost substrate (byproduct resulting from the production of biodiesel). Among the known Trichoccocus species, production of PDO from glycerol was only observed by Trichococcus strain ES5. Although Trichococcus strains share high similarity in their 16S RNA gene, differences at genomic level seem to be much higher and low DNA-DNA hybridization values were observed for the different species7. We have currently a collaboration with the German Collection of Microorganisms (DSMZ) and the Joint Genome Institute (JGI) for sequencing the genomes of all the thus far isolated Tricochoccus species, and the comparative analysis of these genomes will be done within this PhD project. Further on, proteomics will be performed to study specific physiological properties. For example, one fascinating physiological characteristic of Trichococcus species is the capability of growing at very low temperatures – specifically, Trichococcus patagoniensis8 can be a targeted species to study this feature as it can grow at -5oC by producing a mucous substance that covers the cells

Comparative genomic and physiological analysis of Trichococcus species

Axenic cultures of Trichococcus species will be analysed, with a special emphasis on Trichococcus strain ES5 that can produce PDO from glycerol. PDO can be used to compose polyesters and polyurethanes or it can be used as solvent and an antifreeze/protective agent5,6; its production from glycerol is attractive because this is a low cost substrate (byproduct resulting from the production of biodiesel). Among the known Trichoccocus species, production of PDO from glycerol was only observed by Trichococcus strain ES5. Although Trichococcus strains share high similarity in their 16S RNA gene, differences at genomic level seem to be much higher and low DNA-DNA hybridization values were observed for the different species7. We have currently a collaboration with the German Collection of Microorganisms (DSMZ) and the Joint Genome Institute (JGI) for sequencing the genomes of all the thus far isolated Tricochoccus species, and the comparative analysis of these genomes will be done within this PhD project. Further on, proteomics will be performed to study specific physiological properties. For example, one fascinating physiological characteristic of Trichococcus species is the capability of growing at very low temperatures – specifically, Trichococcus patagoniensis8 can be a targeted species to study this feature as it can grow at -5oC by producing a mucous substance that covers the cells

Comparative genomic and physiological analysis of Trichococcus species

Axenic cultures of Trichococcus species will be analysed, with a special emphasis on Trichococcus strain ES5 that can produce PDO from glycerol. PDO can be used to compose polyesters and polyurethanes or it can be used as solvent and an antifreeze/protective agent5,6; its production from glycerol is attractive because this is a low cost substrate (byproduct resulting from the production of biodiesel). Among the known Trichoccocus species, production of PDO from glycerol was only observed by Trichococcus strain ES5. Although Trichococcus strains share high similarity in their 16S RNA gene, differences at genomic level seem to be much higher and low DNA-DNA hybridization values were observed for the different species7. We have currently a collaboration with the German Collection of Microorganisms (DSMZ) and the Joint Genome Institute (JGI) for sequencing the genomes of all the thus far isolated Tricochoccus species, and the comparative analysis of these genomes will be done within this PhD project. Further on, proteomics will be performed to study specific physiological properties. For example, one fascinating physiological characteristic of Trichococcus species is the capability of growing at very low temperatures – specifically, Trichococcus patagoniensis8 can be a targeted species to study this feature as it can grow at -5oC by producing a mucous substance that covers the cells

Comparative genomic and physiological analysis of Trichococcus species

Axenic cultures of Trichococcus species will be analysed, with a special emphasis on Trichococcus strain ES5 that can produce PDO from glycerol. PDO can be used to compose polyesters and polyurethanes or it can be used as solvent and an antifreeze/protective agent5,6; its production from glycerol is attractive because this is a low cost substrate (byproduct resulting from the production of biodiesel). Among the known Trichoccocus species, production of PDO from glycerol was only observed by Trichococcus strain ES5. Although Trichococcus strains share high similarity in their 16S RNA gene, differences at genomic level seem to be much higher and low DNA-DNA hybridization values were observed for the different species7. We have currently a collaboration with the German Collection of Microorganisms (DSMZ) and the Joint Genome Institute (JGI) for sequencing the genomes of all the thus far isolated Tricochoccus species, and the comparative analysis of these genomes will be done within this PhD project. Further on, proteomics will be performed to study specific physiological properties. For example, one fascinating physiological characteristic of Trichococcus species is the capability of growing at very low temperatures – specifically, Trichococcus patagoniensis8 can be a targeted species to study this feature as it can grow at -5oC by producing a mucous substance that covers the cells

Comparative genomic and physiological analysis of Trichococcus species

Axenic cultures of Trichococcus species will be analysed, with a special emphasis on Trichococcus strain ES5 that can produce PDO from glycerol. PDO can be used to compose polyesters and polyurethanes or it can be used as solvent and an antifreeze/protective agent5,6; its production from glycerol is attractive because this is a low cost substrate (byproduct resulting from the production of biodiesel). Among the known Trichoccocus species, production of PDO from glycerol was only observed by Trichococcus strain ES5. Although Trichococcus strains share high similarity in their 16S RNA gene, differences at genomic level seem to be much higher and low DNA-DNA hybridization values were observed for the different species7. We have currently a collaboration with the German Collection of Microorganisms (DSMZ) and the Joint Genome Institute (JGI) for sequencing the genomes of all the thus far isolated Tricochoccus species, and the comparative analysis of these genomes will be done within this PhD project. Further on, proteomics will be performed to study specific physiological properties. For example, one fascinating physiological characteristic of Trichococcus species is the capability of growing at very low temperatures – specifically, Trichococcus patagoniensis8 can be a targeted species to study this feature as it can grow at -5oC by producing a mucous substance that covers the cells