Population Characteristics of Human-Commensal Rodents Present in Households from Mérida, Yucatán, México

Anthropocommensal rodents live in close proximity to humans in many habitats around the world. They are a threat to public health because of the pathogens they carry. Recent studies in Mérida, Yucatán, México, have shown that commensal rodents harbor potential zoonotic pathogens such as bacteria, helminths, and viruses. In this study, we describe reproductive and demographic parameters of house mice and black rats present in households from Mérida, Yucatán, México, a municipality located in a tropical region in southern México. Rodents were trapped in 142 households within the municipality of Mérida from 2011 to 2014. A total of 832 rodents were trapped, constituting 556 (66.8%) house mice (Mus musculus) and 276 (33.2%) black rats (Rattus rattus). The sex ratio in mice was different than parity, while in rats it was 1:1. Mice in the weight class 8.1–12 g were most abundant in both females (52.9%) and males (57.2%). On the other hand, rats weighing ≤ 40 g (25% of females and 28.6% of males) were most abundant. The percentage of pregnancy in mice was 46.7%, whereas in rats it was 21.3%. The mean number of embryos was 4.7 and 5.8 in mice and rats, respectively. This study provides relevant demographic information on the ecology of commensal rodents from a tropical region of Latin America. We consider that our findings could be useful as a first step toward understanding the ecological behavior of rodent populations in tropical regions

Metabarcoding: A Powerful Yet Still Underestimated Approach for the Comprehensive Study of Vector-Borne Pathogen Transmission Cycles and Their Dynamics

The implementation of sustainable control strategies aimed at disrupting the transmission of vector-borne pathogens requires a comprehensive knowledge of the vector ecology in the different eco-epidemiological contexts, as well as the local pathogen transmission cycles and their dynamics. However, even when focusing only on one specific vector-borne disease, achieving this knowledge is highly challenging, as the pathogen may exhibit a high genetic diversity and multiple vector species or subspecies and host species may be involved. In addition, the development of the pathogen and the vectorial capacity of the vectors may be affected by their midgut and/or salivary gland microbiome. The recent advent of Next-Generation Sequencing (NGS) technologies has brought powerful tools that can allow for the simultaneous identification of all these essential components, although their potential is only just starting to be realized. We present a metabarcoding approach that can facilitate the description of comprehensive host-pathogen networks, integrate important microbiome and coinfection data, identify at-risk situations, and disentangle the transmission cycles of vector-borne pathogens. This powerful approach should be generalized to unravel the transmission cycles of any pathogen and their dynamics, which in turn will help the design and implementation of sustainable, effective, and locally adapted control strategies

Perceptions of rodent-associated problems: an experience in urban and rural areas of Yucatan, Mexico

Rodents are a threat to agriculture and homes, and are a public health risk. Local perceptions about rodents and the damage they cause are vital, as a first step, to the design and implementation of rodent control or educational programs. A total of 111 interviews were conducted in two urban neighborhoods and two rural villages in Yucatan, Mexico. More than 90% of the interviewed inhabitants perceived rodents as a problem. The fear of rodents (57%), damage to food and stocks (56%), and damage to clothes (34%), were the most cited problems. In the urban neighborhoods, the use of rodent control methods was more frequent (57%) than in the villages (33%) in this study. In addition, the percentage of damage to domestic appliances was lower in villages (10%) than in neighborhoods (33%). Our preliminary results suggest that rodent pests represent a threat to human health and to human food security in the studied sites.Fil: Panti May, Jesús Alonso. Universidad Autónoma de Yucatán; MéxicoFil: Sodá Tamayo, Lorenzo. Universidad Autónoma de Yucatán; MéxicoFil: Gamboa Tec, Naivy. Universidad Autónoma de Yucatán; MéxicoFil: Cetina Franco, Rosy. Universidad Autónoma de Yucatán; MéxicoFil: Cigarroa Toledo, Nohemi. Universidad Autónoma de Yucatán; MéxicoFil: Machaín Williams, Carlos. Universidad Autónoma de Yucatán; MéxicoFil: Robles, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Hernández Betancourt, Silvia F.. Universidad Autónoma de Yucatán; Méxic