DNA-barcoding revela la existencia de un posible nuevo género del complejo Laurencia (Rodophyta, Ceramiales) en las islas Canarias

XIX Simposio de Botánica Criptogámica, Las Palmas de Gran Canaria, 24-28 de junio de 2013.Basado en análisis morfológicos y moleculares, en la actualidad el complejo Laurencia (Rhodophyta, Ceramiales) incluye seis géneros (Chondrophycus, Laurencia, Laurenciella, Osmundea, Palisada y Yuzurua). El objetivo principal de este estudio preliminar es valorar a nivel molecular la posible existencia de un nuevo género dentro del complejo Laurencia presente en las Islas Canarias, así como establecer posibles relaciones filogenéticas entre éste y otros taxones del complejo citados en la Macaronesia

A molecular perspective of the Laurencia complex (Ceramiales, Rhodophyta) in Macaronesia region

IV Congress of Marine Sciences. Las Palmas de Gran Canaria, June 11th to 13th 2014.In the present study, we undertook an integrative approach, using molecular data to assess the diversity of the Laurencia complex in Macaronesian islands (Azores, Madeira and Canary Islands) where speciation events are supposedly common leading to a high endemism. Identification of species of the Laurencia complex based on anatomical and morphological characters is extremely difficult due to phenotypic plasticity and overlaps in many morphological characters. As a consequence, among the 28 species reported so far from these Macaronesian archipelagos, 14 species records have been regarded as doubtful. We used DNA barcode data (mitochondrial COI gene and partial nuclear LSU marker) as a tool for species delimitation. A third marker (rbcL gene) was also studied and phylogenetic analyses were carried out using the three independent markers as well as the combined data set, in the aim to infer the phylogenetic relationships and biogeographic affinities of members of the complex from Macaronesia. Our results proved the usefulness of the DNA barcode markers for uncovering several putative new species of the Laurencia complex in Macaronesia and phylogenetic results revealed the existence of a potential new genus present in Canary Islands, which adds to the six pre-existing genera: Laurencia, Osmundea, Chondrophycus, Palisada, Yuzurua and Laurenciella

A DNA barcode approach of the Laurencia complex (Ceramiales, Rodophyta) in the tropical and subtropical Atlantic ocean

10th International Phycological Congress, Orlando, Florida, USA, 4-10 de agosto 2013.The diversity of the Laurencia complex is being assessed in tropical and subtropical Atlantic by an international cooperation project involving Brazil, Mexico, Spain (Canary Islands), Portugal (Azores and Madeira) and USA (Florida) on the base of molecular data allied to a detailed morphological study of species. The diversity of the complex was analyzed for the first time for the Atlantic Ocean, including specimens from all five localities, using the plastid 23S rRNA gene (UPA) which has been investigated as potential DNA Barcode marker for photosynthetic eukaryotes. The mitochondrial cytochrome c oxidase I gene (COI-5P) was also used as DNA barcode for the same set of species, and the rbcL gene was used for phylogenetic inferences. The range of genetic variation was compared for the three markers. The UPA proved to be more conserved; however, the same genetic groups were resolved with each of the three markers confirming the six genera currently established for the complex: Chondrophycus, Laurencia, Laurenciella, Palisada, Osmundea and Yuzurua

La resolución de problemas no rutinarios en el aula de primaria y secundaria. Un estudio con profesores

En este trabajo se analizan los resultados obtenidos al aplicar un cuestionario antes del inicio de un curso para profesores dirigido a tres tipos de docentes. Se trata de que expresen su opinión sobre el uso de la resolución de problemas en el aula de Matemáticas. Este análisis reveló similitudes y diferencias entre las opiniones que tienen estos profesores sobre cómo afrontan la resolución de problemas, cuáles son los aspectos pedagógicos que involucran su desarrollo en el aula y la forma de construir las matemáticas cuando la usan

Preliminary study on diversity of the Laurentia Complex Rhodophita, Ceramiales in the Macaronesian region assessed by DNA barcoding and phylogenetic inferences

International Simposio in Marine Science 2012 [III Simposio Internacional de Ciencias del Mar], Marine Biology Section. Cádiz, Andaluzia, Espanha, 24-27 de Janeiro

The presence and suppressive activity of Myeloid-Derived Suppressor Cells are potentiated after Interferon-ß treatment in a murine model of Multiple Sclerosis

Multiple sclerosis (MS) is an autoimmune demyelinating disease of the human central nervous system (CNS), mainly affecting young adults. Among the immunomodulatory disease modifying treatments approved up to date to treat MS, IFN-β remains to be one of the most widely prescribed for the Relapsing-Remitting (RR) variant of the disease, although its mechanism of action is still partially understood. RR-MS variant is characterized by phases with increasing neurological symptoms (relapses) followed by periods of total or partial recovery (remissions), which implies the existence of immunomodulatory agents to promote the relapsing-to-remitting transition. Among these agents, it has been described the immunosuppressive role of a heterogeneous population of immature myeloid cells, namely the myeloid-derived suppressor cells (MDSCs) during the clinical course of the experimental autoimmune encephalomyelitis (EAE), the most used MS model to study RRMS. However, it is still unknown how the current MS disease modifying treatments, e.g. IFN- β, affects to MDSCs number or activity. Our present results show that a single injection of IFN-β at the onset of the clinical course reduces the severity of the EAE, enhancing the presence of MDSCs within the smaller demyelinated areas. Moreover, the single dose of IFN-β promotes MDSC immunosuppressive activity both in vivo and in vitro, augmenting T cell apoptosis. Finally, we show that IFN-ß preserves MDSC immaturity, preventing their differentiation to mature and less suppressive myeloid cell subsets. Taking together, all these data add new insights into the mechanism of IFN-β treatment in EAE and point to MDSCs as a putative endogenous mediator of its beneficial role in this animal model of MS.This work was supported by the Spanish Ministerio de Economía, Industria y Competitividad-MINEICO (SAF2012-40023; SAF2016-77575-R; RD12-0032-12; RD16-0015-0019; PI15-00963; PI18/00357, partially financed by F.E.D.E.R.: European Union, “Una Manera de hacer Europa”), the Spanish Research Council/Consejo Superior de Investigaciones Científicas-CSIC (CSIC-2015201023), ADEM-TO (Spain) and ARSEP Foundation (France). DC, RL-G and IM-D were financed by SESCAM; CM-J holds a predoctoral Research Training contract from MINEICO (BES-2013-062630, –associated to SAF2012-40023 and PI15-00963) and is currently hired under SAF2016-77577-R. Dr. Clemente's group was sponsored by Aciturri Aeronáutica SLU, Vesuvius Ibérica LA and Fundación Galletas Coral

DNA-barcoding revela la existencia de un posible nuevo género del complejo <i>Laurencia</i> (Rodophyta, Ceramiales) en Las Islas Canarias

International audienc

Diversidade do complexo Laurencia (Ceramiales. Rhodophyta) no Atlântico Tropical e Subtropical baseado em "DNA barcoding" e inferências filogenéticas.

XIV Congresso Brasileiro de Ficologia. Estação Cabo Branco - João Pessoa, Paraíba, 17-21 de Julho

Diversity of the Laurencia complex (Ceramiales, Rhodophyta) in Lusitanian Macronesia

10th International Phycological Congress, Orlando, Florida, USA, 4-10 de agosto 2013.Our aim was to investigate the diversity of the Laurencia complex in the Azores, Madeira, Salvagens and Canary Islands, Atlantic oceanic archipelagos, which belong to the same ecoregion, Lusitanian province of the Macaronesian region. We assessed the species limits among members of the complex using DNA barcode data (partial sequences of mitochondrial COI and nuclear LSU markers). The information obtained from the analyses of COI and LSU sequences was consistent; both markers displayed adequate signal for the delineation of species. In addition, preliminary phylogenetic analyses were completed using three independent markers (rbcL, LSU, and COI) as well as the combined data set, in the aim to infer the phylogenetic relationships and biogeographic affinities of members of the complex from Macaronesia. Our results revealed the existence of several putative new species and a potential new genus included in the Laurencia complex. Prior the proposition of new taxa, more thorough morphological and molecular analyses of specimens of type localities, together with the results obtained in our study, are essential to assign the available taxonomic name to the different species uncovered in our studies

A combined barcode and morphological approach to the systematics and biogeography of Laurencia pyramidalis and Laurenciella marilzae (Rhodophyta)

Copyright © 2014 British Phycological Society.In the present study we undertook an integrative approach, using both morphological and molecular data (COI-5P + rbcL), to assess the presence of Laurencia pyramidalis in Lusitanian Macaronesia.We studied type material of L. pyramidalis from the herbarium of the Naturalis Biodiversity Center, the Netherlands, and designated a lectotype and syntypes. Vegetative and reproductive features of L. pyramidalis were observed and we included a specimen from the type locality in our molecular analyses.We also investigated the geographical distribution of Laurenciella marilzae, a species recently described from the Canary Islands. Barcode sequences (COI-5P and rbcL) were generated for L. pyramidalis from the type locality (Normandy, France), the Azores, Madeira and the Canary Islands, and for L. marilzae from its type locality (Tenerife, Canary Islands), the Azores and Brazil