29 research outputs found
Neuroadaptive events in the offspring induced by maternal voluntary ingestion of hypertonic sodium solution: brain neurochemical system programming
Fil: Vivas, Laura. Universidad Nacional de C贸rdoba. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; Argentina.Fil: Macchione, Ana Fabiola. Universidad Nacional de C贸rdoba. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; Argentina.Osmoregulatory mechanisms can be vulnerable to electrolyte and/or endocrine
environmental changes during the perinatal period, differentially programming the
developing offspring and affecting them even in adulthood. The aim of our study was to
evaluate whether the perinatal availability of hypertonic sodium solution [PM-Na] from
the pregnancy until one month old of the offspring may induce a differential
programming of osmoregulatory mechanisms in adult offspring. We have studied the
perinatal manipulation [PM] effects on adult water and sodium intake, brain cell activity
and gene expression changes induced by different osmotic challenges: acute sodium
depletion (ASD) and systemic sodium overload (SSO).Fil: Vivas, Laura. Universidad Nacional de C贸rdoba. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; Argentina.Fil: Macchione, Ana Fabiola. Universidad Nacional de C贸rdoba. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; Argentina.Biolog铆a del Desarroll
Reclutamiento de procesos de aprendizaje a nivel fetal a trav茅s de la intoxicaci贸n et铆lica: Programaci贸n de la apetencia hacia la droga y de disrupciones en la plasticidad respiratoria
El etanol durante el embarazo ejerce efectos teratog茅nicos; en otras palabras, la droga m谩s consumida en el mundo posee la capacidad de inducir o aumentar la incidencia de malformaciones cong茅nitas cuando se administra o act煤a durante la organog茅nesis. El Espectro de Desordenes Fetales del Alcohol incluye una serie de alteraciones anatomo-fisiol贸gicas que ponen en peligro el desarrollo del individuo prenatalmente expuesto a la droga. Sin embargo, este rango de malformaciones y consecuencias negativas a nivel neurocomportamental no incluye aun a los fen贸menos de programaci贸n fetal respecto a la afinidad hacia la droga y en relaci贸n a los trastornos funcionales que impactan sobre la neuroplasticidad respiratoria. Como ser谩 discutido en el presente simposio, ambos fen贸menos est谩n 铆ntimamente ligados a tempranos procesos de aprendizaje derivados de la exposici贸n a la droga. Al aunar la literatura precl铆nica, cl铆nica y epidemiol贸gica, se observa que infantes y adolescentes prenatalmente expuestos al psicotr贸pico, exhiben una marcada predisposici贸n a consumir la droga. En especies altriciales incluyendo la humana, esta afinidad et铆lica ha sido corroborada al examinar el reconocimiento y la preferencia hacia atributos olfato-gustativos de la droga y patrones de consumo voluntario de la misma en funci贸n de la exposici贸n et铆lica prenatal. Recientes investigaciones de orden precl铆nicas, realizadas durante periodos ontog茅nicos an谩logos al segundo y tercer trimestres de gestaci贸n humana, han enfatizado la noci贸n que la programaci贸n de la apetencia et铆lica est谩 determinada por la interacci贸n de al menos tres factores. Los mismos son: (i) el procesamiento fetal de los atributos quimiosensiorales de la droga que est谩n presentes en el contexto intrauterino, (ii) el reclutamiento et铆lico de efectos reforzantes a nivel cerebral que tambi茅n implica una progresiva sensibilizaci贸n hacia la propiedades motivacionales de la droga y (iii) un proceso de aprendizaje asociativo derivado de la contingencia entre los dos primeros factores antes mencionados. Este fen贸meno de aprendizaje Pavloviano es dependiente del reclutamiento del sistema opioid茅rgico cerebral. El mismo modula tanto la sensibilidad hacia las aristas reforzantes positivas de la droga como en referencia a sus propiedades reforzantes negativas; en otros t茅rminos, su acci贸n ansiol铆tica. Asimismo, los estudios indican un significativo rol del principal metabolito del etanol (acetaldeh铆do) que r谩pidamente se genera y acumula en el cerebro a trav茅s de la acci贸n metab贸lica del sistema de catalasas cerebral. Recientes investigaciones indican que este metabolito desencadena tempranos efectos reforzantes positivos e incluso procesos de orden ansiol铆ticos. En forma concomitante con estos procesos de orden motivacionales, el etanol y el acetaldeh铆do, ejercen efectos depresores sobre la respiraci贸n fetal y neonatal. Estudios precl铆nicos y cl铆nicos indican que la neuroplasticidad respiratoria derivada de la exposici贸n temprana al psicotr贸pico tambi茅n es dependiente de procesos de aprendizaje no asociativos (sensibilizaci贸n) y asociativos. En su conjunto, los estudios aqu铆 presentados enfatizan la noci贸n que, incluso al considerarse dosis subteratog茅nicas del etanol, la experiencia temprana con el psicotr贸pico recluta tempranas capacidades sensoriales y de aprendizaje que programan patol贸gicamente al organismo en t茅rminos de su reactividad fisiol贸gica y comportamental hacia la droga.Fil: Macchione, Ana Fabiola. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; Argentina. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; ArgentinaFil: Anunziata, Florencia. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Abate, Paula. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; Argentina. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaFil: Molina, Juan Carlos. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; Argentina. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaXVIII Reuni贸n Nacional; VII Encuentro Internacional de la Asociaci贸n Argentina de Ciencias del ComportamientoMar del PlataArgentinaAsociaci贸n Argentina de Ciencias del ComportamientoInstituto de Psicolog铆a B谩sica, Aplicada y Tecnolog铆aUniversidad Nacional de Mar del Plat
Learning experiences comprising central ethanol exposure in rat neonates: Effects upon respiratory plasticity and the brain catalase system
Fetal ethanol (EtOH) exposure represents a risk factor for the Sudden Infant Death Syndrome and it鈥檚 early effects upon respiration also promotes hypoxic ischemic consequences. This study analyzes central ethanol鈥檚 effects upon breathing plasticity during a stage in the development of the rat equivalent to the 3rd human gestational trimester. The study not only analyzed ethanol鈥檚 unconditioned breathing effects but also how they are regulated by learning processes. Taking into account that ethanol is primarily metabolized in the brain via the catalase system, we examined the effects of early history with the drug upon the activity of this enzymatic system. During postnatal days 3, 5 and 7 (PDs 3-7) pups either received intracisternal (i.c.) administrations of vehicle or ethanol (300 mg%). They were subsequently exposed to a whole body plethysmograph under normoxia. The apparatus was scented or not with the ethanol odor. The presence of the odorant increased breathing rates. The state of intoxication attenuated the onset of apneas; a phenomenon indicative of an antianxiety effect of the drug given the state of arousal caused by the novel environment, maternal deprivation and the stress of i.c. administrations. At PD9, pups were tested while sober under sequential air conditions (initial-normoxia, hypoxia and recovery-normoxia). Once again the plethysmograh was unscented or contained EtOH odor. Prior experience with the scented chamber associated with EtOH鈥檚 central effects elicited a conditioned isodirectional response relative to the onset of apneas previously observed during PDs 3-7. Yet, prior history with the drug exacerbated the onset of apneas when pups were defied with hypoxia. Following this test, pups ingested 0.8 g/kg of absolute EtOH and their brains were analyzed to determine catalase activity. Pre-exposure to EtOH鈥檚 central effects paired with the odor of the drug resulted in heightened enzymatic activity. The results indicate that central EtOH accumulation may exert antianxiety effects that attenuate apneic disruptions but that also has long-lasting effects upon respiratory plasticity under hypoxia. Most importantly, these effects appear to be related with how the brain catalase system reacts to the presence of EtOH in accordance with the nature of prior experiences with the drug.Fil: Trujillo, Ver贸nica. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; Argentina. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; ArgentinaFil: Albretch, Paula Alejandra. Universidad Nacional de C贸rdoba. Facultad de Ciencias Qu铆micas. Departamento de Farmacolog铆a; ArgentinaFil: Deza Ponzio, Romina. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Farmacolog铆a Experimental de C贸rdoba. Universidad Nacional de C贸rdoba. Facultad de Ciencias Qu铆micas. Instituto de Farmacolog铆a Experimental de C贸rdoba; Argentina. Universidad Nacional de C贸rdoba. Facultad de Ciencias Qu铆micas. Departamento de Farmacolog铆a; ArgentinaFil: Virgolini, Miriam Beatriz. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Farmacolog铆a Experimental de C贸rdoba. Universidad Nacional de C贸rdoba. Facultad de Ciencias Qu铆micas. Instituto de Farmacolog铆a Experimental de C贸rdoba; Argentina. Universidad Nacional de C贸rdoba. Facultad de Ciencias Qu铆micas. Departamento de Farmacolog铆a; ArgentinaFil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; Argentina. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; ArgentinaIX International Meeting of the Latin American Society for Biomedical Research on Alcoholism (LASBRA): Determinants of Alcoholism: Bridging the gap between epidemiological and basic researchC贸rdobaArgentinaLatin American Society for Biomedical Research on Alcoholis
Developmental programing of thirst and sodium appetite
Thirst and sodium appetite are the sensations responsible for the motivated behaviors of water and salt intake, respectively, and both are essential responses for the maintenance of hydromineral homeostasis in animals. These sensations and their related behaviors develop very early in the postnatal period in animals. Many studies have demonstrated several pre- and postnatal stimuli that are responsible for the developmental programing of thirst and sodium appetite and, consequently, the pattern of water and salt intake in adulthood in need-free or need-induced conditions. The literature systematically reports the involvement of dietary changes, hydromineral and cardiovascular challenges, renin-angiotensin system and steroid hormone disturbances, and lifestyle in these developmental factors. Therefore, this review will address how pre- and postnatal challenges can program lifelong thirst and sodium appetite in animals and humans, as well as which neuroendocrine substrates are involved. In addition, the possible epigenetic molecular mechanisms responsible for the developmental programing of drinking behavior, the clinical implications of hydromineral disturbances during pre- and postnatal periods, and the developmental origins of adult hydromineral behavior will be discussed.Fil: Mecawi, Andre S.. Universidade Federal Rural do Rio de Janeiro; Brasil. University of Malaysia; MalasiaFil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Nu帽ez, Paula. Universidad de Oviedo; Espa帽aFil: Perillan, Carmen. Universidad de Oviedo; Espa帽aFil: Reis, Luis C.. Universidade Federal Rural do Rio de Janeiro; BrasilFil: Vivas, Laura Marta. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Arguelles, Juan. Universidad de Oviedo; Espa帽
Sensitization to ethanol鈥檚 disruptive effects upon early breathing plasticity associated with hypoxia and hypercapnia
Ethanol (EtOH) consumption during pregnancy and lactation represents a risk factor related with the Sudden Infant Death Syndrome (SIDS). This phenomenon has promoted research linking prenatal EtOH effects on the respiratory system during early ontogeny. It should also be noted that prolonged episodes of neonatal respiratory depression represent a risk factor in terms of hypoxic-ischemic effects with negative consequences on brain development. In a first study during postnatal day (PD) 9 we analyzed the impact of different doses of EtOH (0.0, 0.75, 1.37 or 2.0 g/kg) upon the respiratory response and the potential psychomotor effects in pup rats pre-exposed or not to 2.0 g/kg of EtOH during PDs 3-7. At PD 9 animals were also subjected to sequential air conditions defined as initial normoxia, hypoxia and recovery normoxia. In a second study we analyzed the blood of animals only exposed to 0.0 or 2.0g/kg of EtOH during PDs 3-9 (not subjected to a hypoxic event). The aim was to examine if mere intoxication with a moderate dose of EtOH is capable of modifying blood metabolic patterns associated with hypoxia or hypercapnia. In the first study during PDs 3-7 EtOH exerted a depressant effect upon breathing frequencies. These animals also showed a progressive sensitization effect relative to the depressant effects of the drug and lesser levels of apneas. At PD 9 dose dependent respiratory depressions were observed when pups were challenged with a hypoxic event. Independently from prior experience with EtOH, drug treatment at PD 9 significantly disrupted respiratory frequencies particularly during the hypoxic and the recovery normoxia phases. Respiratory disorders triggered by these air conditions have been implicated in the pathophysiology of SIDS. These results show that breathing plasticity is disrupted during a critical stage where respiratory alterations may lead to hypoxiaassociated syndromes that endanger brain development. In terms of psychomotor activity, animals exposed to 2.0 g/kg of EtOH at PD 9 showed heightened duration and frequency of grooming. In the second study animals exposed at least one time to EtOH exhibited lower pH and higher CO2 than animals that were never exposed to EtOH. This results suggest metabolic acidosis probably due to EtOH-related hypercapnia during a vulnerable stage in development relative to SIDS.Fil: Anunziata, Florencia. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; Argentina. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaFil: Angulo Alcalde, Asier. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Trujillo, Mar铆a Ver贸nica. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Wille-bille, Aranza. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Amigone, Jos茅 Luis. Hospital Privado de C贸rdoba. Laboratorio de Bioqu铆mica Cl铆nica; ArgentinaFil: Molina, Juan Carlos. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; Argentina. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaIX LASBRA International Meeting: Determinants of Alcoholism: bridging the gap between epidemiologic and basic researchC贸rdobaArgentinaLatin American Society for Biomedical Research on Alcoholis
Omega 3 mitigation on deficient hypoxic-ventilatory response (HVR) induced by moderate ethanol doses in an animal model equivalent to the third trimester of human pregnancy
Maternal ethanol (EtOH) intake during pregnancy and lactation is a highly frequent "social" behaviour in Argentine, expounding fetus or neonates to moderate EtOH intoxication through amniotic fluid and maternal milk. Early exposure to EtOH triggers in the organism a spectrum of neurobehavioral dysfunctions affecting, also, the breathing response. Hypoxia acts as an environmental stressor eliciting breathing adaptations, like Hypoxic Ventilatory Responses-HVR, that may be altered by the EtOH exposure. One of the detrimental effects of early EtOH exposure is the reduction of the Omega3-O3 levels in the CNS. Experiment 1: Pups were intoxicated with 2.0g/kg or 0.0g/kg of EtOH, ig at postnatal days (PDs) 3-5-7-9. At PD9, also were subjected to a hypoxic event [5 min of initial normoxia, followed by 3 episodes of hypoxia (O2 8%) of 5 min, separated by periods of recovery-normoxia of the same duration]. Breathing frequencies and apneas were recorded in a whole body plethysmograph at PDs 3-5-7 x 5 min and at PD9 x 35 min of test. Experiment 2: The same experimental protocol of EtOH exposure at PDs 3-5-7-9 was employed. However, at PDs 3-5-7, pups received 0.0 or 720 mg/kg O3 (ig), 20 min after EtOH administration. At PDs 5 and 7, EtOH-intoxicated pups elicited a breathing depression that was not affected by the O3 administration. During the hypoxic challenge, EtOH-exposed pups expressed a significant breathing depression during initial normoxia and also at each hypoxic event (i.e. a reduced HVR) relative to it expressed in vehicle-exposed pups (p<0.05). Preliminary results indicated a restoration of the HVR in pups exposed to EtOH with O3. A significant increase in the number of apneas in EtOH intoxicated pups was observed after the 2nd and 3rd hypoxic events. At these periods, a protective O3 effect was observed by a reduction in the number of apneas in EtOH-intoxicated pups. These results seem to indicate a mitigation due to O3 upon deleterious EtOH effects in the neonatal HVR. Funding by MINCyT-Cba; FONCyT and SECyT-UNC.Fil: Segovia, Marisol Magali. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaFil: Balaszczuk, Veronica. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; Argentina. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; ArgentinaFil: Macchione, Ana Fabiola. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; Argentina. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaLXVII Reuni贸n Anual de la Sociedad Argentina de Investigaci贸n Cl铆nica; LXX Reuni贸n Anual de la Sociedad Argentina de Inmunolog铆a; 3er Congreso Franco Argentino de Inmunolog铆a y Reuni贸n Anual 2022 de la Sociedad Argentina de Fisiolog铆aMar del PlataArgentinaSociedad Argentina de Investigaci贸n Cl铆nicaSociedad Argentina de Inmunolog铆aSociedad Argentina de Fisiolog铆
Exposici贸n temprana a dosis moderadas de etanol y omega 3: Efectos sobre la respiraci贸n y la degeneraci贸n celular en el SNC de neonatos de rata
La exposici贸n temprana a EtOH desencadena en el organismo un espectro de disfunciones neuroconductuales afectando la respuesta respiratoria frente a hipoxia, reduciendo los niveles de Omega3-O3 en el SNC e incrementando la tasa de degeneraci贸n neuronal. El objetivo de este estudio fue analizar, en un modelo experimental equivalente al tercer trimestre gestacional humano, los efectos del etanol y la acci贸n protectiva del O3 sobre la respuesta respiratoria frente a hipoxia y el n煤mero de c茅lulas picn贸ticas-CP en 谩reas del tronco encef谩lico involucradas en dicha respuesta. Se intoxicaron neonatos con 2,0 o 0,0 g/kg EtOH (ig) en los d铆as postnatales-DP 3-5-7-9 [Experimento-1] y adem谩s en los DP3-5-7, las cr铆as recibieron0,0 o 720 mg/kg O3 (ig), 20 minutos despu茅s de la administraci贸n de EtOH[Experimento-2]. Al DP9, las cr铆as fueron sometidas a un evento de hipoxia intermitente (EHI) x 35 minutos. Posteriormente, se colectaron los cerebros y se realiz贸 una tinci贸n con Azul de Toluidina al 0,25% para la evaluaci贸n del n煤mero de CP en 谩reas cerebrales de inter茅s. i-La exposici贸n temprana a EtOH indujo depresiones respiratorias durante la EHI; mientras que el O3 tuvo un efecto protector restaurando dicha respuesta. Ii-La exposici贸n a EtOH increment贸 significativamente el n煤mero de CP en el n煤cleo del tracto solitario-NTS, rafe magnus y pallidus. iii-La exposici贸n a EIH aument贸 significativamente el n煤mero de CP en NTS y rafe magnus. iv-En rafe obscurus se encontr贸 una acci贸n sin茅rgica entre ambos tratamientos (EtOH+EHI). v-S ehall贸 un efecto protector del O3 en NTS ante el desarrollo de CP en animales expuestos a EHI. En conclusi贸n, existir铆a un efecto protectivo del O3 sobre los efectos del EtOH durante un EHI evidenciado por una mejora en la ventilaci贸n y una disminuci贸n en el n煤mero de CP en 谩reas implicadas en dicha mejora.Fil: Segovia, Marisol Magali. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaFil: Balaszczuk, Veronica. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; Argentina. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaFil: Macchione, Ana Fabiola. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; Argentina. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaII Jornadas de investigaci贸n sobre consumo de sustancias psicoactivas en UruguayMontevideoUruguayUniversidad de la Rep煤blica. Facultad de Ciencias Veterinarias. Departamentos de Biociencia
Differential effects of the ethanol acute or chronic exposure on the early hypoxic ventilatory response (hvr) in rat neonates
Early ethanol exposure disrupts neonatal respiratory patterns and it has been suggested as risk factor associated with the Sudden Infant Death Syndrome. Ambient hypoxia acts as an environmental stressor eliciting breathing adaptations that may be altered by the EtOH exposure. However, the specific effects induce by chronic, acute or the combination of them EtOH intoxication are not clearly understood. In an animal model equivalent to the 3rd human gestational trimester, breathing frequencies and apneas were recorded against an intermittent hypoxic experience as a function of EtOH pre-exposure and/or acute EtOH intoxication. Pups pre-exposed to 0.0 or 2.0g/kg of EtOH (DPs 3-5-7, ig) were evaluated at DP9 in sobriety-0.0g/kg- or under the state of EtOH intoxication-2.0g/kg-. Breathing rates and apneas were recorded through whole body plexismography during 35 minutes [5 min of initial normoxia, followed by 3 episodes of hypoxia (O2 8%) of 5 min, separated by periods of recovery-normoxia of the same duration].First acute EtOH intoxication diminished the hypoxic ventilatory response (HVR) during the test (p=0.034) relative to it expressed in pups never intoxicated. The prior experience with the drug significantly modified the HVR patterns, as follow: in sobriety, EtOH pre-exposed pups exhibited a depressed HVR relative to vehicle pre-exposed pups. On the contrary, under the state of intoxication, EtOH pre-exposed pups elicited an exacerbated HVR when were defied by hypoxia respect to vehicle pre-exposed pups. With regard to apneas, an increase in the number of apneas was triggered by both, the first acute EtOH intoxication or by the history with the drug in sober pups (p= 0.017). In summary, specific HVR alterations and apneic episodes occurrence were observed in neonates depending on the type of EtOH exposure received (acute or chronic). These results emphasize the complexity of the disruptive EtOH effects upon breathing at this early and critical stage of development.Fil: Macchione, Ana Fabiola. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; Argentina. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaFil: Segovia, Marisol Magal铆. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaFil: Molina, Juan Carlos. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; Argentina. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Abate, Paula. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; Argentina. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaReuni贸n de Sociedades De Biociencias 2021; Lxvi Reuni贸n Anual de la Sociedad Argentina de Investigaci贸n Cl铆nica (Saic); Lxix Reuni贸n Anual de la Sociedad Argentina de Inmunolog铆a (Sai); Liii Reuni贸 Anual de La Asociaci贸n Argentina de Farmacolog铆a Experimental (Aafe); Xi Reuni贸n Anual de la Asociaci贸n Argentina de Nanomedicinas (Nanomed-Ar)Buenos AiresArgentinaSociedad Argentina de Inmunolog铆aSociedad Argentina de Investigaci贸n Cl铆nicaAsociaci贸n Argentina de Farmacolog铆a ExperimentalSociedad Argentina de Nanomedicina
Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats
EtOH麓s effects upon respiration are attributed to central respiratory network disruptions, especially in the medullary serotonin (5HT) system. 5HT2A/2C receptors are involved in the reduction of the phrenic nerve activity and breathing depression. We hypothesize that early EtOH preexposure alters neonatal respiration through the 5HT system麓s plasticity. Here, we evaluated breathing rates and the relative expression of 5HT 2A and 2C receptors in the brainstem as a function of EtOH preexposure in neonates. Pups received i.g administrations of 2.0 or 0.0g/kg EtOH at postnatal days (PD) 3, 5 and 7. At PD 9, breathing frequencies were recorded under normoxia or hypoxia. Brainstems were collected to quantified relative mRNA expression of 5HT 2A and 2C receptors, by qPCR. Under normoxia, EtOH preexposed pups (preEtOH) exhibited high 5HT2A expression levels and breathing depressions. An opposite phenomenon was observed in preEtOH pups tested under hypoxia. An exacerbated hyperventilation associated with low 5HT2A expression levels was found. No significant differences were found in 5HT2C expression levels. These results together with our previous findings that show changes in the raphe obscurus activation patterns, suggest that a brief EtOH preexposure is enough to induce 5HT system?s plasticity, disturbing neonatal breathing. The 5HT components mismatch may be associated with breathing disruptions commonly observed in human neonates, such as Sudden Infant Death Syndrome.Fil: Macchione, Ana Fabiola. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; ArgentinaFil: Anunziata, Florencia. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Sahonero, Milagros. Universidad Nacional de C贸rdoba. Facultad de Psicolog铆a; ArgentinaFil: Trujillo, Ver贸nica. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaXXXIV Reuni贸n Anual de la Sociedad Argentina de NeurocienciasVilla Carlos PazArgentinaSociedad Argentina de Investigaci贸n en Neurociencia
Comparison of dipsogenic responses of adult rat offspring as a function of different perinatal programming models
The perinatal environment interacts with the genotype of the developing organism resulting in a unique phenotype through a developmental or perinatal programming phenomenon. However, it remains unclear how this phenomenon differentially affects particular targets expressing specific drinking responses depending on the perinatal conditions. The main goal of the present study was to compare the dipsogenic responses induced by different thirst models as a function of two perinatal manipulation models, defined by the maternal free access to hypertonic sodium solution and a partial aortic ligation (PAL-W/Na) or a sham-ligation (Sham-W/Na). The programmed adult offspring of both perinatal manipulated models responded similarly when was challenged by overnight water dehydration or after a sodium depletion showing a reduced water intake in comparison to the non-programmed animals. However, when animals were evaluated after a body sodium overload, only adult Sham-W/Na offspring showed drinking differences compared to PAL and control offspring. By analyzing the central neurobiological substrates involved, a significant increase in the number of Fos + cells was found after sodium depletion in the subfornical organ of both programmed groups and an increase in the number of Fos + cells in the dorsal raphe nucleus was only observed in adult depleted PAL-W/Na. Our results suggest that perinatal programming is a phenomenon that differentially affects particular targets which induce specific dipsogenic responses depending on matching between perinatal programming conditions and the osmotic challenge in the latter environment. Probably, each programmed-drinking phenotype has a particular set point to elicit specific repertoires of mechanisms to reestablish fluid balance.Fil: Dadam, Florencia Maria. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Amigone, Jorge Luis. Hospital Privado Universitario de C贸rdoba; ArgentinaFil: Vivas, Laura Marta. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra. Universidad Nacional de C贸rdoba. Instituto de Investigaci贸n M茅dica Mercedes y Mart铆n Ferreyra; ArgentinaFil: Macchione, Ana Fabiola. Universidad Nacional de C贸rdoba. Instituto de Investigaciones Psicol贸gicas. - Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - C贸rdoba. Instituto de Investigaciones Psicol贸gicas; Argentin