Development of a 3D coculture system to study adipocyte and lymph node cell interactions

We have developed a long term 3-dimensional coculture system with adipocytes and lymph node cells for the purpose of investigating interactions between these cells in vitro. Present experimental work with the culture system is aimed at introducing lymph node cells, in proportions similar to those found in intact lymph nodes, among differentiated adipocytes and observing interactions and the establishment of a spatial relationship between them. Co-cultures will be used to investigate the lymph node adipocyte interactions following immune stimulation (lipopolysaccharide treatment) measuring production of inflammatory mediators (cytokines) and lipolytic activity

The Role of Adipokines in Understanding the Associations between Obesity and Depression

Objective. Two major causes of disability, major depression and obesity, share overlapping psychosocial and pathophysiological etiologies. Studies are now focused on biological mechanisms linking the two illnesses, and there is interest in the role that adipokines may have in mediating the association between obesity and depression. We reviewed the literature to look at what is currently known about this association, focusing on the adipokines leptin, adiponectin, and resistin. Methods. A MEDLINE search, citing articles from 1966 onward, supplemented by a review of bibliographies, was conducted to identify relevant studies. Results. This paper identified plausible pathways underlying a link between adipokines and depression. Only a few studies have yet been conducted specifically examining these biomarkers in patients with depression, but the results are intriguing. Conclusion. This paper is one of the first to examine the association between adipokines and depression. It provides an overview of the physiological role of adipokines and summarizes the data suggesting that they may be dysregulated in major depression. This area of research may become increasingly important as new treatment strategies are developed

Stellar Activity and its Implications for Exoplanet Detection on GJ 176

We present an in-depth analysis of stellar activity and its effects on radial velocity (RV) for the M2 dwarf GJ 176 based on spectra taken over 10 years from the High Resolution Spectrograph on the Hobby-Eberly Telescope. These data are supplemented with spectra from previous observations with the HIRES and HARPS spectrographs, and V- and R-band photometry taken over 6 years at the Dyer and Fairborn observatories. Previous studies of GJ 176 revealed a super-Earth exoplanet in an 8.8-day orbit. However, the velocities of this star are also known to be contaminated by activity, particularly at the 39-day stellar rotation period. We have examined the magnetic activity of GJ 176 using the sodium I D lines, which have been shown to be a sensitive activity tracer in cool stars. In addition to rotational modulation, we see evidence of a long-term trend in our Na I D index, which may be part of a long-period activity cycle. The sodium index is well correlated with our RVs, and we show that this activity trend drives a corresponding slope in RV. Interestingly, the rotation signal remains in phase in photometry, but not in the spectral activity indicators. We interpret this phenomenon as the result of one or more large spot complexes or active regions which dominate the photometric variability, while the spectral indices are driven by the overall magnetic activity across the stellar surface. In light of these results, we discuss the potential for correcting activity signals in the RVs of M dwarfs.Comment: Accepted for publication in Ap

Searching for the expelled hydrogen envelope in Type I supernovae via late-time H-alpha emission

We report the first results from our long-term observational survey aimed at discovering late-time interaction between the ejecta of hydrogen-poor Type I supernovae and the hydrogen-rich envelope expelled from the progenitor star several decades/centuries before explosion. The expelled envelope, moving with a velocity of ~10 -- 100 km s$^{-1}$, is expected to be caught up by the fast-moving SN ejecta several years/decades after explosion depending on the history of the mass-loss process acting in the progenitor star prior to explosion. The collision between the SN ejecta and the circumstellar envelope results in net emission in the Balmer-lines, especially in H-alpha. We look for signs of late-time H-alpha emission in older Type Ia/Ibc/IIb SNe having hydrogen-poor ejecta, via narrow-band imaging. Continuum-subtracted H-alpha emission has been detected for 13 point sources: 9 SN Ibc, 1 SN IIb and 3 SN Ia events. Thirty-eight SN sites were observed on at least two epochs, from which three objects (SN 1985F, SN 2005kl, SN 2012fh) showed significant temporal variation in the strength of their H-alpha emission in our DIAFI data. This suggests that the variable emission is probably not due to nearby H II regions unassociated with the SN, and hence is an important additional hint that ejecta-CSM interaction may take place in these systems. Moreover, we successfully detected the late-time H-alpha emission from the Type Ib SN 2014C, which was recently discovered as a strongly interacting SN in various (radio, infrared, optical and X-ray) bands.Comment: 8 pages, 7 figures, accepted in Ap

The Pinhole/Occulter Facility

A large occulting system in space can be used for high resolution X-ray observations and for large aperture coronagraphic observations in visible and UV light. The X-ray observations can combine high angular resolution in hand (10 keV) X-radiation with the high sensitivity of a multiple pinhole camera, and can permit sensitive observations of bremsstrahlung from nonthermal particles in the corona. The large aperture coronagraphs have two major advantages: high angular resolution and good photon collection. This will permit observations of small scale structures in the corona for the first time and will give sufficient counting rates above the coronal background rates for sensitive diagnostic analysis of intensities and line profiles for coronal structures in the solar wind acceleration region. The technical basis for performing observations with a large occulting system in these three wavelength ranges is described as well as a pinhole/occulter facility presently being considered for Spacelab. Some indications about future developments are included

Material-based Stratification

A simple probability model was applied to detection sampling in a room or space in which different surface materials are present. The model assesses the overall detection capability when the sampling and analytical methods have different performance properties for the different materials. The results suggest that some common sampling strategies may not be ideal. In particular: (1) In a single room or area that includes different surface types with different detection properties, do not use a single sampling grid with a common spacing throughout. (2) If it is known or strongly suspected that one material has better detection properties than the other, place all samples on that material. (3) When it is completely unknown which material has the better detection properties, allocate the samples equally between them

Beryllium Health and Safety Committee Data Reporting Task Force

On December 8, 1999, the Department of Energy (DOE) published Title 10 CFR 850 (hereafter referred to as the Rule) to establish a chronic beryllium disease prevention program (CBDPP) to: {sm_bullet} reduce the number of workers currently exposed to beryllium in the course of their work at DOE facilities managed by DOE or its contractors, {sm_bullet} minimize the levels of, and potential for, expos exposure to beryllium, and {sm_bullet} establish medical surveillance requirements to ensure early detection of the disease