The inferior turbinate flap in skull base reconstruction

BACKGROUND: As the indications for expanded endonasal approaches continue to evolve, alternative reconstructive techniques are needed to address increasingly complex surgical skull base defects. In the absence of the nasoseptal flap, we describe our experience with the posterior pedicle inferior turbinate flap (PPITF) in skull base reconstruction. DESIGN: Case series. SETTING: Academic tertiary care centre. METHODS: Patients who underwent reconstruction of the skull base with the PPITF were identified. Medical records were reviewed for demographic, presentation, treatment, follow-up, surgical and outcomes data. MAIN OUTCOME MEASURES: Flap survival, adequacy of seal, and complications. RESULTS: Two patients with residual/recurrent pituitary adenomas met the inclusion criteria. The nasoseptal flap was unavailable in each case due to a prior septectomy. Salvage of the original nasoseptal flap was not possible, as it did not provide adequate coverage of the resultant defect due to contraction from healing. All PPITFs healed uneventfully and covered the entire defect. No complications were observed in the early post-operative period. Endoscopic techniques and limitations of the PPITF are also discussed. CONCLUSIONS: Our clinical experience supports the PPITF to be a viable alternative for reconstruction of the skull base in the absence of the nasoseptal flap

Death and Display in the North Atlantic: The Bronze and Iron Age Human Remains from Cnip, Lewis, Outer Hebrides

YesThis paper revisits the series of disarticulated human remains discovered during the 1980s excavations of the Cnip wheelhouse complex in Lewis. Four fragments of human bone, including two worked cranial fragments, were originally dated to the 1st centuries BC/AD based on stratigraphic association. Osteoarchaeological reanalysis and AMS dating now provide a broader cultural context for these remains and indicate that at least one adult cranium was brought to the site more than a thousand years after the death of the individual to whom it had belonged

Isotopic evidence of increasing water abundance and lake hydrological change in Old Crow Flats, Yukon, Canada

Lake-rich northern permafrost landscapes are sensitive to changing climate conditions, but ability to track real-time and potentially multiple hydrological responses (e.g. lake expansion, drawdown, drainage) is challenging due to absence of long-term, sustainable monitoring programs in these remote locations. Old Crow Flats (OCF), Yukon, is a Ramsar Wetland of International Importance where concerns about low water levels and their consequences for wildlife habitat and traditional ways of life prompted multidisciplinary studies during the International Polar Year (2007–2008) and led to the establishment of an aquatic ecosystem monitoring program. Here, we report water isotope data from 14 representative thermokarst lakes in OCF, the foundation of the monitoring program, and time-series of derived metrics including the isotope composition of input waters and evaporation-to-inflow ratios for a 13 year period (2007–2019). Although the lakes spanned multiple hydrological categories (i.e. rainfall-, snowmelt- and evaporation-dominated) based on initial surveys, well-defined trends from application of generalized additive models and meteorological records reveal that lakes have become increasingly influenced by rainfall, and potentially waters from thawing permafrost. These sources of input have led to more positive lake water balances. Given the documented role of rainfall in causing thermokarst lake drainage events in OCF and elsewhere, we anticipate increased vulnerability of lateral water export from OCF. This study demonstrates the value of long-term isotope-based monitoring programs for identifying hydrological consequences of climate change in lake-rich permafrost landscapes.Northern Scientific Training Program of Polar Knowledge Canad

Multicolour correlative imaging using phosphor probes

Correlative light and electron microscopy exploits the advantages of optical methods, such as multicolour probes and their use in hydrated live biological samples, to locate functional units, which are then correlated with structural details that can be revealed by the superior resolution of electron microscopes. One difficulty is locating the area imaged by the electron beam in the much larger optical field of view. Multifunctional probes that can be imaged in both modalities and thus register the two images are required. Phosphor materials give cathodoluminescence (CL) optical emissions under electron excitation. Lanthanum phosphate containing thulium or terbium or europium emits narrow bands in the blue, green and red regions of the CL spectrum; they may be synthesised with very uniform-sized crystals in the 10- to 50-nm range. Such crystals can be imaged by CL in the electron microscope, at resolutions limited by the particle size, and with colour discrimination to identify different probes. These materials also give emissions in the optical microscope, by multiphoton excitation. They have been deposited on the surface of glioblastoma cells and imaged by CL. Gadolinium oxysulphide doped with terbium emits green photons by either ultraviolet or electron excitation. Sixty-nanometre crystals of this phosphor have been imaged in the atmospheric scanning electron microscope (JEOL ClairScope). This probe and microscope combination allow correlative imaging in hydrated samples. Phosphor probes should prove to be very useful in correlative light and electron microscopy, as fiducial markers to assist in image registration, and in high/super resolution imaging studies

Spontaneous Inter-layer Coherence in Double-Layer Quantum-Hall Systems I: Charged Vortices and Kosterlitz-Thouless Phase Transitions

At strong magnetic fields double-layer two-dimensional-electron-gas systems can form an unusual broken symmetry state with spontaneous inter-layer phase coherence. In this paper we explore the rich variety of quantum and finite-temperature phase transitions associated with this broken symmetry. We describe the system using a pseudospin language in which the layer degree-of-freedom is mapped to a fictional spin 1/2 degree-of-freedom. With this mapping the spontaneous symmetry breaking is equivalent to that of a spin 1/2 easy-plane ferromagnet. In this language spin-textures can carry a charge. In particular, vortices carry e/2 electrical charge and vortex-antivortex pairs can be neutral or carry charge e. We derive an effective low-energy action and use it to discuss the charged and collective neutral excitations of the system. We have obtained the parameters of the Landau-Ginzburg functional from first-principles estimates and from finite-size exact diagonalization studies. We use these results to estimate the dependence of the critical temperature for the Kosterlitz-Thouless phase transition on layer separation.Comment: 56 pages, 19 figures available upon request at [email protected]. RevTex 3.0. IUCM94-00

Electrodynamics of a Clean Vortex Lattice

We report on a microscopic evaluation of electrodynamic response for the vortex lattice state of a model s-wave superconductor. Our calculation accounts self-consistently for both quasiparticle and order parameter response and establishes the collective nature of linear response in the clean limit. We discuss the effects of homogeneous and inhomogeneous pinning on the optical conductivity and the penetration depth, and comment on the relationship between macroscopic and local penetration depths. We find unexpected relationships between pinning arrangements and conductivity due to the strongly non-local response.Comment: 4 pages, 2 figure

A retinoblastoma allele that is mutated at its common E2F interaction site inhibits cell proliferation in gene-targeted mice

The retinoblastoma protein (pRB) is best known for regulating cell proliferation through E2F transcription factors. In this report, we investigate the properties of a targeted mutation that disrupts pRB interactions with the transactivation domain of E2Fs. Mice that carry this mutation endogenously (Rb1δG) are defective for pRB-dependent repression of E2F target genes. Except for an accelerated entry into S phase in response to serum stimulation, cell cycle regulation in Rb1δG/δG mouse embryonic fibroblasts (MEFs) strongly resembles that of the wild type. In a serum deprivation-induced cell cycle exit, Rb1δG/δG MEFs display a magnitude of E2F target gene derepression similar to that of Rb1-/- cells, even though Rb1δG/δG cells exit the cell cycle normally. Interestingly, cell cycle arrest in Rb1δG/δG MEFs is responsive to p16 expression and gamma irradiation, indicating that alternate mechanisms can be activated in G1 to arrest proliferation. Some Rb1δG/δG mice die neonatally with a muscle degeneration phenotype, while the others live a normal life span with no evidence of spontaneous tumor formation. Most tissues appear histologically normal while being accompanied by derepression of pRB-regulated E2F targets. This suggests that non- E2F-, pRB-dependent pathways may have a more relevant role in proliferative control than previously identified. © 2014, American Society for Microbiology

Metabolic variability in seafloor brines revealed by carbon and sulphur dynamics

Brine fluids that upwell from deep, hot reservoirs below the sea bed supply the sea floor with energy-rich substrates and nutrients that are used by diverse microbial ecosystems. Contemporary hypersaline environments formed by brine seeps may provide insights into the metabolism and distribution of microorganisms on the early Earth or on extraterrestrial bodies. Here we use geochemical and genetic analyses to characterize microbial community composition and metabolism in two seafloor brines in the Gulf of Mexico: an active mud volcano and a quiescent brine pool. Both brine environments are anoxic and hypersaline. However, rates of sulphate reduction and acetate production are much higher in the brine pool, whereas the mud volcano supports much higher rates of methane production. We find no evidence of anaerobic oxidation of methane, despite high methane fluxes at both sites. We conclude that the contrasting microbial community compositions and metabolisms are linked to differences in dissolved-organic-matter input from the deep subsurface and different fluid advection rates between the two sites. DOI: 10.1038/NGEO47

The effects of insulin resistance on individual tissues: an application of a mathematical model of metabolism in humans

Whilst the human body expends energy constantly, the human diet consists of a mix of carbohydrates and fats delivered in a discontinuous manner. To deal with this sporadic supply of energy, there are transport, storage and utilisation mechanisms, for both carbohydrates and fats, around all tissues of the body. Insulin-resistant states such as type 2 diabetes and obesity are characterised by reduced efficiency of these mechanisms. Exactly how these insulin-resistant states develop, for example whether there is an order in which tissues become insulin resistant, is an active area of research with the hope of gaining a better overall understanding of insulin resistance. In this paper we use a previously derived system of 12 first-or der coupled differential equations that describe the transport between, and storage in, different tissues of the human body. We briefly revisit the derivation of the model before parametrising the model to account for insulin resistance. We then solve the model numerically, separately simulating each individual tissue as insulin resistant, and discuss and compare these results, drawing three main conclusions. The implications of these results are in accordance with biological intuition. First, insulin resistance in a tissue creates a knock-on effect on the other tissues in the body, whereby they attempt to compensate for the reduced efficiency of the insulin resistant tissue. Secondly, insulin resistance causes a fatty liver; and the insulin resistance of tissues other than the liver can cause fat to accumulate in the liver. Finally, although insulin resistance in individual tissues can cause slightly reduced skeletal-muscle metabolic flexibility, it is when the whole body is insulin resistant that the biggest effect on skeletal muscle flexibility is see