“Orange-Peeling” An innovative fixed tooth preparation technique

This report describes an innovative technique of crown preparation, called “Orange-Peeling”, a straight forward technique valuable for increasing accuracy and speed of the procedure. The concept of this new technique evolves about undermining surface enamel by cutting through it from the side rather than grinding throughout the entire hard surface enamel

Determination of some nutrientions (PO43-, NO3-, NO2-, NH4+) In the waters of the Dreikish dam

The periodic analytical study of Dreikish dam water - Dreikish region - Syria was carried out by sampling water samples every two months in 2017-2018 for monitoring sites inside the lake area and for the water received from multiple sampling sites. The study included the determination of some ions that feed the water of the Dreikish dam such as phosphate, nitrate, nitrite, and ammonium ions by UV/VIS spectroscopy. The concentration of phosphate ions was ranged between (0.00-0.26) mg / l, with a percentage relative standard deviation not exceed than5.00%. The concentration of nitrate ion was ranged between (0.3-12.0) mg / l,  with a percentage relative standarddeviation of only 0.5%. The concentration of nitrite ion was ranged between (0.01-0.71) mg / l,  with a percentage relative standarddeviation of only 5.00%. The concentration of Ammonium ion was ranged between (0.00-1.51) mg / l,  with a percentage relative standarddeviation of only 5.00%. Nitrite concentration was exceeded its permissible limit of drinking water at the dam site in the beginning of the summer of 2017. Ammonium values were ​​exceeded thepermissible limit of drinking water at the dump site in the ninth month and at the lake tail site in the seventh month of 2017.  

Modulation of Human Time Processing by Subthalamic Deep Brain Stimulation

Timing in the range of seconds referred to as interval timing is crucial for cognitive operations and conscious time processing. According to recent models of interval timing basal ganglia (BG) oscillatory loops are involved in time interval recognition. Parkinsońs disease (PD) is a typical disease of the basal ganglia that shows distortions in interval timing. Deep brain stimulation (DBS) of the subthalamic nucleus (STN) is a powerful treatment of PD which modulates motor and cognitive functions depending on stimulation frequency by affecting subcortical-cortical oscillatory loops. Thus, for the understanding of BG-involvement in interval timing it is of interest whether STN-DBS can modulate timing in a frequency dependent manner by interference with oscillatory time recognition processes. We examined production and reproduction of 5 and 15 second intervals and millisecond timing in a double blind, randomised, within-subject repeated-measures design of 12 PD-patients applying no, 10-Hz- and ≥130-Hz-STN-DBS compared to healthy controls. We found under(re-)production of the 15-second interval and a significant enhancement of this under(re-)production by 10-Hz-stimulation compared to no stimulation, ≥130-Hz-STN-DBS and controls. Milliseconds timing was not affected. We provide first evidence for a frequency-specific modulatory effect of STN-DBS on interval timing. Our results corroborate the involvement of BG in general and of the STN in particular in the cognitive representation of time intervals in the range of multiple seconds

Neuroanatomical background and functional considerations for stereotactic interventions in the H fields of Forel

The H fields of Forel constitute an intricate neuroanatomical structure that occupies a central position within the posterior subthalamus. Anatomically, it features a dense concentration of fiber bundles including corticofugal, pallidothalamic, cerebellothalamic and other projections that connect functionally relevant areas of the brain. Functionally, the fields of Forel are embedded within the cortico-striato-thalamo-cortical circuit and constitute the main link between the striatopallidal system and the thalamocortical network. Given the current understanding of basal ganglia involvement in movement disorders and neuropsychiatric disease we sought to investigate the H fields of Forel as a potential target in stereotactic functional neurosurgery. Although historically recognized in the treatment of movement disorders, behavioral disorders and epilepsy, the significance of the H fields is considerably diminished today receiving only little attention. Owing to the current lack of reviews addressing the anatomical and functional organization of Forel's fields, we aim to deliver an up-to-date overview of the H fields in this paper. We investigate the complex neuroanatomy and describe the passage of the various fiber systems that course through the posterior subthalamus. We revise the role of Forel's fields in the current context of our understanding of cortico-basal ganglia circuitry and discuss the historic relevance of Forel's fields during the lesional era. Finally, we provide an outlook regarding the potential of deep brain stimulation in close proximity and within the H fields of Forel

Investigating the Existence of Ribosomal Protein L5 Gene in Syrian Strain of Leishmania tropica Genome: Sequencing It and Evaluating Its Immune Response as DNA Vaccine

Cutaneous leishmaniasis in Syria is caused mainly by Leishmania tropica. It represents a serious health problem, which has aggravated further after the civil war in the country. Until now, there are no effective protective strategies, safe therapy, or efficacious vaccine to protect from this infection. DNA vaccines represent a promising approach for achieving protection against leishmaniasis. The L5 ribosomal protein plays fundamental roles in the assembly process of the ribosome subunits, so this study has chosen the ribosomal protein L5 gene to design a DNA vaccine against Leishmania tropica infection. After proving the existence of the ribosomal protein L5 gene in a Syrian strain of Leishmania tropica (LCED Syrian 01), it was sequenced and cloned into a pCI plasmid, and the designed DNA vaccine was administered to BALB/c mice. The protective response was evaluated by measuring lesion development in immunized BALB/c mice for 6 weeks after challenging mice with the parasite. RT-qPCR was used to quantify IL-12, IFN-γ, and IL-4 in draining lymph nodes (DLNs) of immunized mice. In the final week, the parasite burden was determined in footpad lesions and local draining lymph nodes (DLNs). This study demonstrated the presence and expression of the ribosomal protein L5 gene in the Syrian strain of Leishmania tropica promastigotes. The sequence of the ribosomal protein cDNA L5 gene was determined and published in Genbank. The gene size was 918 bp. Expression was also demonstrated at the level of cDNA. This study also demonstrated that vaccination with the ribosomal protein L5 gene induces TH1 response in immunized mice. This response prevents the partial development of a skin lesion of Leishmania

Impedance learning adaptive super‐twisting control of a robotic exoskeleton for physical human‐robot interaction

Abstract This study addresses two issues about the interaction of the upper limb rehabilitation robot with individuals who have disabilities. The first step is to estimate the human's target position (also known as TPH). The second step is to develop a robust adaptive impedance control mechanism. A novel Non‐singular Terminal Sliding Mode Control combined with an adaptive super‐twisting controller is being developed to achieve this goal. This combination's purpose is to provide high reliability, continuous performance tracking of the system's trajectories. The proposed adaptive control strategy reduces matched dynamic uncertainty while also lowering chattering, which is the sliding mode's most glaring issue. The proposed TPH is coupled with adaptive impedance control with the use of a Radial Basis Function Neural Network, which allows a robotic exoskeleton to simply track the desired impedance model. To validate the approach in real‐time, an exoskeleton robot was deployed in controlled experimental circumstances. A comparison study has been set up to show how the adaptive impedance approach proposed is better than other traditional controllers

A Retrospective Case Series of High-Frequency Spinal Cord Stimulation (HF10-SCS) in Neurogenic Bladder Incontinence

BACKGROUND Current treatment options for bladder disorders of neurogenic etiology often leave unsatisfactory results. Therefore, new and effective treatments must be investigated. High-frequency spinal cord stimulation (HF-SCS) at 10 kHz has proven to be effective in the treatment of refractory chronic back and leg pain. OBJECTIVE To evaluate the efficacy of HF-SCS at 10 kHz in alleviating lower urinary tract dysfunction and bladder incontinence in 5 patients with underlying neurological disease or spinal cord injury, through retrospective study. METHODS Urodynamic parameters such as voiding frequency, residual volume, episodes of incontinence, and the patients' subjective impression impairment of life were assessed and compared pre- and postoperatively. Reduction in pain intensity was assessed as change on the Numeric Rating Scale (NRS). RESULTS All 5 patients had significantly positive outcomes. Episodes of leakage per day improved by 83% on average. Quality of life questionnaires and subjective bother scale revealed an improvement of 36% and 57%, respectively. Individual symptoms among the patient group such as residual volume also responded to the treatment as well. Mean pain NRS of 8.6 cm was reduced to 3.9 cm (55%) at 6 mo follow-up. CONCLUSION HF-SCS at 10 kHz significantly alleviated symptoms of neurogenic bladder incontinence in patients suffering from neurological disease or spinal cord injury. However, larger and prospective, randomized studies are necessary to make a clear statement regarding the efficacy of this therapy in lower urinary tract dysfunction and bladder incontinence