106 research outputs found
Generation and dietary modulation of anti-inflammatory electrophilic omega-3 fatty acid derivatives
Dietary ω-3 polyunsaturated fatty acids (PUFAs) decrease cardiovascular risk via suppression of inflammation. The generation of electrophilic α,β-unsaturated ketone derivatives of the ω-3 PUFAs docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA) in activated human macrophages is catalyzed by cyclooxygenase-2 (Cox-2). These derivatives are potent pleiotropic anti-inflammatory signaling mediators that act via mechanisms including the activation of Nrf2- dependent phase 2 gene expression and suppression of pro-inflammatory NF-κB-driven gene expression. Herein, the endogenous generation of ω-3 PUFAs electrophilic ketone derivatives and their hydroxy precursors was evaluated in human neutrophils. In addition, their dietary modulation was assessed through a randomized clinical trial. Methods: Endogenous generation of electrophilic omega-3 PUFAs and their hydroxy precursors was evaluated by mass spectrometry in neutrophils isolated from healthy subjects, both at baseline and upon stimulation with calcium ionophore. For the clinical trial, participants were healthy adults 30-55 years of age with a reported EPA+DHA consumption of ≤ 300 mg/day randomly assigned to parallel groups receiving daily oil capsule supplements for a period of 4 months containing either 1.4 g of EPA+DHA (active condition, n = 24) or identical appearing soybean oil (control condition, n = 21). Participants and laboratory technicians remained blinded to treatment assignments. Results: 5-lypoxygenase-dependent endogenous generation of 7-oxo-DHA, 7-oxo-DPA and 5-oxo-EPA and their hydroxy precursors is reported in human neutrophils stimulated with calcium ionophore and phorbol 12-myristate 13-acetate (PMA). Dietary EPA+DHA supplementation significantly increased the formation of 7-oxo-DHA and 5-oxo-EPA, with no significant modulation of arachidonic acid (AA) metabolite levels. Conclusions: The endogenous detection of these electro.©2014 Cipollina et al
Modeling Translation in Protein Synthesis with TASEP: A Tutorial and Recent Developments
The phenomenon of protein synthesis has been modeled in terms of totally
asymmetric simple exclusion processes (TASEP) since 1968. In this article, we
provide a tutorial of the biological and mathematical aspects of this approach.
We also summarize several new results, concerned with limited resources in the
cell and simple estimates for the current (protein production rate) of a TASEP
with inhomogeneous hopping rates, reflecting the characteristics of real genes.Comment: 25 pages, 7 figure
Origin and ascent history of unusually crystal-rich alkaline basaltic magmas from the western Pannonian Basin
The last eruptions of the monogenetic Bakony-Balaton Highland Volcanic Field
(western Pannonian Basin, Hungary) produced unusually crystal- and xenolith-rich
alkaline basalts which are unique among the alkaline basalts of the Carpathian-
Pannonian Region. Similar alkaline basalts are only rarely known in other volcanic
fields of the world. These special basaltic magmas fed the eruptions of two closely
located volcanic centres: the BondorĂł-hegy and the FĂĽzes-tĂł scoria cone. Their
uncommon enrichment in diverse crystals produced unique rock textures and modified
original magma compositions (13.1-14.2 wt.% MgO, 459-657 ppm Cr, 455-564 ppm Ni
contents).
Detailed mineral-scale textural and chemical analyses revealed that the BondorĂł-hegy
and FĂĽzes-tĂł alkaline basaltic magmas have a complex ascent history, and that most
of their minerals (~30 vol.% of the rocks) represent foreign crystals derived from
different levels of the underlying lithosphere. The most abundant xenocrysts, olivine,
orthopyroxene, clinopyroxene and spinel, were incorporated from different regions and
rock types of the subcontinental lithospheric mantle. Megacrysts of clinopyroxene and
spinel could have originated from pegmatitic veins / sills which probably represent
magmas crystallized near the crust-mantle boundary. Green clinopyroxene xenocrysts
could have been derived from lower crustal mafic granulites. Minerals that crystallized
in situ from the alkaline basaltic melts (olivine with Cr-spinel inclusions, clinopyroxene,
plagioclase, Fe-Ti oxides) are only represented by microphenocrysts and overgrowths
on the foreign crystals. The vast amount of peridotitic (most common) and mafic
granulitic materials indicates a highly effective interaction between the ascending
magmas and wall rocks at lithospheric mantle and lower crustal levels. However,
fragments from the middle and upper crust are absent from the studied basalts,
suggesting a change in the style (and possibly rate) of magma ascent in the crust.
These xenocryst- and xenolith-rich basalts yield divers tools for estimating magma
ascent rate that is important for hazard forecasting in monogenetic volcanic fields.
According to the estimated ascent rates, the BondorĂł-hegy and FĂĽzes-tĂł alkaline
basaltic magmas could have reached the surface within hours to few days, similarly to
the estimates for other eruptive centres in the Pannonian Basin which were fed by
"normal" (crystal- and xenolith-poor) alkaline basalts
Implementing the WHO integrated tool to assess quality of care for mothers, newborns and children: results and lessons learnt from five districts in Malawi
Benefits, risks and clinical practices related to augmentation of labour with oxytocin in low- and lower-middle-income countries:A systematic review
Replication of Bacteriophage M-13. Replication of Bacteriophage M-13 Positive Role of Gene-5 Protein in Single-Strand-DNA Synthesis
RELATIONSHIP OF TEMPERATURE TO INAPPARENT INFECTION OF HELA CELLS WITH HERPES SIMPLEX VIRUS
Reasons for performing a caesarean section in public hospitals in rural Bangladesh
Background
It is estimated that 18.5 million Caesarean Sections (CS) are conducted annually worldwide and about one-third of them are done without medical indications and described as “unnecessary”. Although developed countries account for most of the rise in the trend of unnecessary CS, more studies report a similar trend in developing countries, putting a strain on existing but limited healthcare resources, jeopardizing families' financial security and presenting a barrier to equitable universal coverage. We examined indications for CS in public hospitals of one district in Bangladesh and explored factors influencing decision to perform the procedure.
Methods
Retrospective review of case notes of 530 women who had CS in 5 public hospitals in Thakurgaon District of Bangladesh. Key Informant Interviews (KII) with 18 service providers to explore factors associated with the decision to perform a CS.
Results
The commonest recorded indications for CS were: previous CS (29.4%), fetal distress (15.7%), cephalo-pelvic disproportion (10.2%), prolonged obstructed labor (8.3%) and post-term dates (7.0%). The majority (68%) of CS were performed as emergency; mainly during daytime working hours. Previous CS and “post-term dates” were common indications for elective CS with “post dates” – the commonest indication for CS in primiparous women. 16.0% of all CS were conducted for cases where alternative forms of care might have been more appropriate. Providers reported not using protocols and evidence based guidelines even though these are available. Pressure from patients and relatives to deliver by CS strongly influenced decision making. External agents from private hospitals receive a financial reward for every CS performed and are present in public hospitals to “lobby” for CS.
Conclusion
Factors other than evidence based practice or the presence of a clear medical indication influence providers’ decision to perform both elective and emergency CS in public hospitals in Bangladesh
- …