Characterization of CMR5c and CMR12a, novel fluorescent Pseudomonas strains from the cocoyam rhizosphere with biocontrol activity

Aim: To screen for novel antagonistic Pseudomonas strains producing both phenazines and biosurfactants that are as effective as Pseudomonas aeruginosa PNA1 in the biocontrol of cocoyam root rot caused by Pythium myriotylum. Material and Results: Forty pseudomonads were isolated from the rhizosphere of healthy white and red cocoyam plants appearing in natural, heavily infested fields in Cameroon. In vitro tests demonstrated that Py. myriotylum antagonists could be retrieved from the red cocoyam rhizosphere. Except for one isolate, all antagonistic isolates produced phenazines. Results from whole-cell protein profiling showed that the antagonistic isolates are different from other isolated pseudomonads, while BOX-PCR revealed high genomic similarity among them. 16S rDNA sequencing of two representative strains within this group of antagonists confirmed their relatively low similarity with validly described Pseudomonas species. These antagonists are thus provisionally labelled as unidentified Pseudomonas strains. Among the antagonists, Pseudomonas CMR5c and CMR12a were selected because of their combined production of phenazines and biosurfactants. For strain CMR5c also, production of pyrrolnitrin and pyoluteorin was demonstrated. Both CMR5c and CMR12a showed excellent in vivo biocontrol activity against Py. myriotylum to a similar level as Ps. aeruginosa PNA1. Conclusion: Pseudomonas CMR5c and CMR12a were identified as novel and promising biocontrol agents of Py. myriotylum on cocoyam, producing an arsenal of antagonistic metabolites. Significance and Impact of the Study: Present study reports the identification of two newly isolated fluorescent Pseudomonas strains that can replace the opportunistic human pathogen Ps. aeruginosa PNA1 in the biocontrol of cocoyam root rot and could be taken into account for the suppression of many plant pathogens

How to implement the organic regulation to increase production & use of organic seed. Policy recommendations for national and regional authorities

Contents Introduction Part One - Policy Measures 1.1 Measures to stimulate organic seed production and use 1.1.1 Training 1.1.2 Subsidies 1.1.3 Access to cleaning facilities for organic seed producers 1.1.4 Organic cultivar trials 1.2 Derogation Rules 1.3 National Annex 1.4 Equivalent cultivars 1.5 Vegetative propagating material 1.6 Expert Groups 1.6.1 Role and mandate of Expert Groups Part Two - Organic Seed Databases 2.1 Upload of seed offers on national databases 2.2 Features and operation of national organic seed databases 2.3 EU Router database Part Three - Alternative sources of organic seed 3.1 Traditional cultivars & farm saved seed 3.2 Populations and Organic Heterogeneous Materia

Organic seed production and use in Hungary

Organic seed production and use in Hungar

Securing Commitments from Stakeholders in 10 EU Member States - The Organic Seed Declaration to Foster Stakeholder Involvement

The new European organic regulation 2018/848 aims to phase out the use of non-organic seeds in organic farming by 2036. At present, achieving this goal in countries with a poorly developed organic seed sector is difficult, and therefore there is a great need to increase organic seed supply by promoting the development of the organic seed sector in Europe. This paper presents a conceptual framework to secure voluntary stakeholder involvement in the process of a gradual increase in the supply of organic seeds for organic farming. Stakeholders showed a high motivation to commit to concrete action points for moving forward. In addition, further actors were involved in the fulfillment of the commitments, a sign of a positive network effect in favor of organic seed production and use. The study indicates application potential and can complement mandatory policy instruments. Further progress monitoring is necessary to ensure that established structures maintain their function, and to keep the shared sense of responsibility alive

European project on improving the competitiveness of organic agriculture by boosting organic seed and plant breeding across Europe (2017 – 2021)

European project on improving the competitiveness of organic agriculture by boosting organic seed and plant breeding across Europe (2017 – 2021

How to implement the organic regulation to increase production & use of organic seed - policy recommendations for national and regional authorities

This booklet for policymakers and stakeholders at the national and regional level describes, based on the results of the LIVESEED project, how to stimulate organic seed production and use, and how to enable organic seed availability on the market. Topics discussed in the booklet include: - policy measures such as training, subsidies, access to facilities and post-release cultivar trials - how to manage the derogation process in combination with the National organic seed databases and an EU router, including the role and mandate of expert groups - the need for adapted cultivars, enabled through alternative seed sources, among which are farm saved seed, populations and organic heterogeneous materia

LIVESEED boosting organic seed and plant breeding across Europe

The European project LIVESEED (www.liveseed.eu) is based on the concept that cultivars adapted to organic systems are key for realizing the full potential of organic agriculture in Europe. LIVESEED will help to establish a level playing field in the organic seed market across Europe, improve the competitiveness of the organic seed and breeding sector, and encourage greater use of organic seeds by farmers. LIVESEED will improve guidelines for cultivar testing and strategies for ensuring seed health. It will develop innovative breeding approaches suited to organic farming. Finally, it will investigate socio-economic aspects relating to the use and production of organic seed and their interaction with relevant (EU) regulations. The LIVESEED project (2017 – 2021) is coordinated by IFOAM EU with FiBL for scientific coordination and consists of 35 partners and 14 third linked parties from 18 European countries. LIVESEED received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 727230 and by the Swiss State Secretariat for Education, Research and Innovation (SERI) under contract number 17.00090. By connecting several networks LIVESEED will combine co construction and exchange of knowledge integrating biological, technical, legal, organisational, financial and political aspects as well as market development to facilitate fast upscaling and outreach of various tailor made socio-technical, evidence based, innovation tracks. LIVESEED will generate (i) a tool box of measures and interventions to match production and demand of organic seed, (ii) validated options for competent authorities to reduce the number of derogation for untreated conventional seed, (iii) develop technical solutions for national databases of organic seed including an interface to a European wide router database to increase the transparency on the availability of organic seed, (iv) analyse in depth formal and informal seed chains, (v) explore various business models including checklists for setting up new multiplication and breeding initiatives, (vi) produce technical factsheet on the best practice guidelines for seed production for major crops including vegetative plant propagation material, and (vii) an develop organic seed quality strategy including quality control of farm saved seeds and efficiency of seed exchange networks. With respect to organic plant breeding LIVESEED will provide new concepts for designing and implementing decentralized breeding initiatives for organic and low-input agriculture through (i) establishing networks across Europe (e.g. for apple, brassica vegetables) and fostering public-private partnerships, (ii) combining farmer, value chain or community based system breeding with functional trait-based and molecular breeding approaches, (iii) identifying trade-offs between resilience traits and sensory and nutritional quality, (iv) developing breeding schemes for heterogeneous cultivars and species mixtures, and (v) elaborating different financing models. Considering the plant as a mega organism, including the above and below ground associated microorganism, has the potential to evoke a paradigm shift in plant breeding, and will be elucidated in case studies

Afstudeerwerk

Voorwoord bij een nieuwe rubriek met afstudeerwerk Het Bulletin KNOB biedt een podium aan zowel ervaren onderzoekers als aanstormend talent. Met een zekere regelmaat publiceren afgestudeerden een artikel op basis van hun masterscriptie of ander onderzoek dat zij aan de universiteit deden. Maar dit is slechts een topje van de ijsberg. Er is immers veel meer werk van studenten dat de aandacht van de lezer verdient. Daarom starten wij in het Bulletin KNOB een rubriek met afstudeerwerk. Eens in de twee jaar vragen wij een aantal pas afgestudeerden van wie de masterthesis of eindopdracht goed is beoordeeld hiervan een synthese te maken. Voor de eerste aflevering van deze rubriek zijn dat degenen die meedongen naar de KNOB Stimuleringsprijs 2021. Het resultaat vindt u in dit nummer: zeventien bijdragen over uiteenlopende onderwerpen, van Cubex-keuken tot herbestemming van industrieel erfgoed en van klimaatverbetering in steden tot Antwerpse zeemanshuizen. De auteurs zijn alumni van universitaire opleidingen op het gebied van architectuur- en stadsgeschiedenis, erfgoed en (landschaps)architectuur in Nederland en België. Met deze rubriek laten wij onze lezers kennisnemen van door studenten verricht onderzoek en bieden wij beginnende onderzoekers en ontwerpers een gelegenheid hun werk in het Bulletin KNOB te presenteren. Onze dank gaat daarbij uit naar collega Jeroen Goudeau, die ons het idee voor een rubriek met afstudeerwerk aan de hand deed. Wij wensen u veel leesplezier en inspiratie toe. De redactieVoorwoord bij een nieuwe rubriek met afstudeerwerk Het Bulletin KNOB biedt een podium aan zowel ervaren onderzoekers als aanstormend talent. Met een zekere regelmaat publiceren afgestudeerden een artikel op basis van hun masterscriptie of ander onderzoek dat zij aan de universiteit deden. Maar dit is slechts een topje van de ijsberg. Er is immers veel meer werk van studenten dat de aandacht van de lezer verdient. Daarom starten wij in het Bulletin KNOB een rubriek met afstudeerwerk. Eens in de twee jaar vragen wij een aantal pas afgestudeerden van wie de masterthesis of eindopdracht goed is beoordeeld hiervan een synthese te maken. Voor de eerste aflevering van deze rubriek zijn dat degenen die meedongen naar de KNOB Stimuleringsprijs 2021. Het resultaat vindt u in dit nummer: zeventien bijdragen over uiteenlopende onderwerpen, van Cubex-keuken tot herbestemming van industrieel erfgoed en van klimaatverbetering in steden tot Antwerpse zeemanshuizen. De auteurs zijn alumni van universitaire opleidingen op het gebied van architectuur- en stadsgeschiedenis, erfgoed en (landschaps)architectuur in Nederland en België. Met deze rubriek laten wij onze lezers kennisnemen van door studenten verricht onderzoek en bieden wij beginnende onderzoekers en ontwerpers een gelegenheid hun werk in het Bulletin KNOB te presenteren. Onze dank gaat daarbij uit naar collega Jeroen Goudeau, die ons het idee voor een rubriek met afstudeerwerk aan de hand deed. Wij wensen u veel leesplezier en inspiratie toe. De redacti