Synthesis of Gold Nanoparticles to Capture Lifelike Proteins: Application on the Multichannel Sensor Array Design

The chemical elements of proteins are similar to that of DNA (e.g., C, H, O, and N), and DNA shows different knotted architectures. So we imagine that proteins may show a wealth of highly complex structures, especially when proteins interact with each other. The imagination was proved by synthesizing gold nanoparticles (GNPs) to capture the lifelike protein structures. The optical responses (i.e., color) of as-prepared GNPs are found to be characteristic to a given protein (or heavy metal ion). Based on the “three colors” principle of Thomas Young, we extracted the red, green, and blue (RGB) alterations of as-synthesized GNPs to fabricate multichannel sensor arrays for proteins (or heavy metal ions) discrimination. The designed multichannel sensor arrays demonstrate possibilities in semiquantitative analysis of multiple analytes (e.g., proteins and heavy metal ions). This work is believed to open new opportunities for GNPs-based label-free sensing

ChatKBQA: A Generate-then-Retrieve Framework for Knowledge Base Question Answering with Fine-tuned Large Language Models

Knowledge Base Question Answering (KBQA) aims to derive answers to natural language questions over large-scale knowledge bases (KBs), which are generally divided into two research components: knowledge retrieval and semantic parsing. However, three core challenges remain, including inefficient knowledge retrieval, retrieval errors adversely affecting semantic parsing, and the complexity of previous KBQA methods. In the era of large language models (LLMs), we introduce ChatKBQA, a novel generate-then-retrieve KBQA framework built on fine-tuning open-source LLMs such as Llama-2, ChatGLM2 and Baichuan2. ChatKBQA proposes generating the logical form with fine-tuned LLMs first, then retrieving and replacing entities and relations through an unsupervised retrieval method, which improves both generation and retrieval more straightforwardly. Experimental results reveal that ChatKBQA achieves new state-of-the-art performance on standard KBQA datasets, WebQSP, and ComplexWebQuestions (CWQ). This work also provides a new paradigm for combining LLMs with knowledge graphs (KGs) for interpretable and knowledge-required question answering. Our code is publicly available.Comment: Preprin

Propofol Anesthesia Increases Long-range Frontoparietal Corticocortical Interaction in the Oculomotor Circuit in Macaque Monkeys.

What we already know about this topicA decrease in frontoparietal functional connectivity has been demonstrated with multiple anesthetic agents, and this decrease has been proposed as a final common functional pathway to produce anesthesia.Two alternative measures of long-range cortical interaction are coherence and phase-amplitude coupling. Although phase-amplitude coupling within frontal cortex changes with propofol administration, the effects of propofol on phase-amplitude coupling between different cortical areas have not previously been reported.What this article tells us that is newUsing a previously published monkey electrocorticography data set, it was found that propofol induced coherent slow oscillations in visual and oculomotor networks made up of cortical areas with strong anatomic projections.Frontal eye field within-area phase-amplitude coupling increased.Contrary to expectations from previous functional connectivity studies, interareal phase-amplitude coupling also increased with propofol.BackgroundFrontoparietal functional connectivity decreases with multiple anesthetics using electrophysiology and functional imaging. This decrease has been proposed as a final common functional pathway to produce anesthesia. Two alternative measures of long-range cortical interaction are coherence and phase-amplitude coupling. Although phase-amplitude coupling within frontal cortex changes with propofol administration, the effects of propofol on phase-amplitude coupling between different cortical areas have not previously been reported. Based on phase-amplitude coupling observed within frontal lobe during the anesthetized period, it was hypothesized that between-lead phase-amplitude coupling analysis should decrease between frontal and parietal leads during propofol anesthesia.MethodsA published monkey electrocorticography data set (N = 2 animals) was used to test for interactions in the cortical oculomotor circuit, which is robustly interconnected in primates, and in the visual system during propofol anesthesia using coherence and interarea phase-amplitude coupling.ResultsPropofol induces coherent slow oscillations in visual and oculomotor networks made up of cortical areas with strong anatomic projections. Frontal eye field within-area phase-amplitude coupling increases with a time course consistent with a bolus response to intravenous propofol (modulation index increase of 12.6-fold). Contrary to the hypothesis, interareal phase-amplitude coupling also increases with propofol, with the largest increase in phase-amplitude coupling in frontal eye field low-frequency phase modulating lateral intraparietal area β-power (27-fold increase) and visual area 2 low-frequency phase altering visual area 1 β-power (19-fold increase).ConclusionsPropofol anesthesia induces coherent oscillations and increases certain frontoparietal interactions in oculomotor cortices. Frontal eye field and lateral intraparietal area show increased coherence and phase-amplitude coupling. Visual areas 2 and 1, which have similar anatomic projection patterns, show similar increases in phase-amplitude coupling, suggesting higher order feedback increases in influence during propofol anesthesia relative to wakefulness. This suggests that functional connectivity between frontal and parietal areas is not uniformly decreased by anesthetics

Co(OH)₂ Nanoflowers Decorated α-NiMoO₄ Nanowires as a Bifunctional Electrocatalyst for Efficient Overall Water Splitting

The development of bifunctional electrocatalysts with high catalytic activity and cyclic stability is an effective method for electrocatalytic water splitting. Herein, a promising hydroxide/oxide Co(OH)2/α-NiMoO4 NWs/CC heterostructure with nanoflowers decorating the nanowires was fabricated on a carbon cloth (CC) substrate via hydrothermal and calcination methods. In contrast to one-dimensional nanomaterials, the interfaces of Co(OH)2 nanoflowers and α-NiMoO4 nanowires on CC provide more active sites for electrocatalytic reactions; therefore, they exhibit obviously enhanced electrocatalytic activities in overall water splitting. Specifically, the Co(OH)2/α-NiMoO4 NWs/CC electrodes exhibit an overpotential of 183.01 mV for hydrogen evolution reaction (HER) and of 170.26 mV for oxygen evolution reactions (OER) at the current density of 10 mA cm−2 in 1.0 M KOH. Moreover, the electrocatalytic oxygen evolution reaction (OER) activity of the Co(OH)2/α-NiMoO4 NWs/CC electrocatalyst was enhanced after long-term stability tests

Correction: Transcriptional and physiological analyses of reduced density in apple provide insight into the regulation involved in photosynthesis.

[This corrects the article DOI: 10.1371/journal.pone.0239737.]

Transcriptional and physiological analyses of reduced density in apple provide insight into the regulation involved in photosynthesis.

Different densities have a great influence on the physiological process and growth of orchard plants. Exploring the molecular basis and revealing key candidate genes for different densities management of orchard has great significance for production capacity improvement. In this study, transcriptome sequencing of apple trees was carried out at three different sampling heights to determine gene expression patterns under high density(HD) and low density(LD) and the physiological indices were measured to determine the effect of density change on plants. As a result, physiological indexes showed that the content of Chlorophyll, ACC, RUBP and PEP in the LD was apparently higher than that in control group(high density, HD). While the content of PPO and AO in the LD was noticeably lower than that in the HD. There were 3808 differentially expressed genes (DEGs) were detected between HD and LD, of which 1935, 2390 and 1108 DEGs were found in the three comparisons(middle-upper, lower-outer and lower-inner), respectively. 274 common differentially expressed genes (co-DEGs) were contained in all three comparisons. Functional enrichment and KEGG pathway analysis found these genes were involved in Carbon fixation in photosynthetic organisms, Circadian rhythm, Photosynthesis - antenna proteins, Photosynthesis, chlorophyll metabolism, Porphyrin, sugar metabolism and so on. Among these genes, LHCB family participated in photosynthesis as parts of photosystem II. In addition, SPA1, rbcL, SNRK2, MYC2, BSK, SAUR and PP2C are involved in Circadian rhythm, the expression of genes related to glycometabolism and hormone signaling pathway is also changed. The results revealed that the decrease of plant density changed the photosynthetic efficiency of leaves and the expression of photosynthesis-related genes, which provide a theoretical basis for the actual production regulation of apples

Zwitterionic polymers-armored amyloid-like protein surface combats thrombosis and biofouling

Proteins, cells and bacteria adhering to the surface of medical devices can lead to thrombosis and infection, resulting in significant clinical mortality. Here, we report a zwitterionic polymers-armored amyloid-like protein surface engineering strategy we called as “armored-tank” strategy for dual functionalization of medical devices. The “armored-tank” strategy is realized by decoration of partially conformational transformed LZM (PCTL) assembly through oxidant-mediated process, followed by armoring with super-hydrophilic poly-2-methacryloyloxyethyl phosphorylcholine (pMPC). The outer armor of the “armored-tank” shows potent and durable zone defense against fibrinogen, platelet and bacteria adhesion, leading to long-term antithrombogenic properties over 14 days in vivo without anticoagulation. Additionally, the “fired” PCTL from “armored-tank” actively and effectively kills both Gram-positive and Gram-negative bacterial over 30 days as a supplement to the lacking bactericidal functions of passive outer armor. Overall, this “armored-tank” surface engineering strategy serves as a promising solution for preventing biofouling and thrombotic occlusion of medical devices

Additional file 1 of Toward efficient and high-fidelity metagenomic data from sub-nanogram DNA: evaluation of library preparation and decontamination methods

Additional file 1: Table S1. Composition and sequences of the synthetic DNA material sequins. Table S2. Characteristics of library construction kits. Table S3. 495 core microbial contaminations at the genus level