Onsite data processing and monitoring for the Daya Bay Experiment

The Daya Bay Reactor Neutrino Experiment started running on September 23, 2011. The offline computing environment, consisting of 11 servers at Daya Bay, was built to process onsite data. With current computing ability, onsite data processing is running smoothly. The Performance Quality Monitoring system (PQM) has been developed to monitor the detector performance and data quality. Its main feature is the ability to efficiently process multi-data-stream from three experimental halls. The PQM processes raw data files from the Daya Bay data acquisition system, generates and publishes histograms via a graphical web interface by executing the user-defined algorithm modules, and saves the histograms for permanent storage. The fact that the whole process takes only around 40 minutes makes it valuable for the shift crew to monitor the running status of all the sub-detectors and the data quality

Understanding Client Reactions in Online Mental Health Counseling

Communication success relies heavily on reading participants' reactions. Such feedback is especially important for mental health counselors, who must carefully consider the client's progress and adjust their approach accordingly. However, previous NLP research on counseling has mainly focused on studying counselors' intervention strategies rather than their clients' reactions to the intervention. This work aims to fill this gap by developing a theoretically grounded annotation framework that encompasses counselors' strategies and client reaction behaviors. The framework has been tested against a large-scale, high-quality text-based counseling dataset we collected over the past two years from an online welfare counseling platform. Our study shows how clients react to counselors' strategies, how such reactions affect the final counseling outcomes, and how counselors can adjust their strategies in response to these reactions. We also demonstrate that this study can help counselors automatically predict their clients' states.Comment: Accept to ACL 2023, oral. For code and data, see https://github.com/dll-wu/Client-Reac

(E)-2-Chloro-N′-(2-hydr­oxy-1-naphthyl­methyl­ene)benzohydrazide

In the structue of the title compound, C18H13ClN2O2, a new Schiff base, the dihedral angle between the benzene and naphthyl ring system mean planes is 22.5 (2)°. The mol­ecule has an E configuration about the C=N bond, and an intra­molecular hydrogen bond involving the hydoxyl substituent on the naphthyl ring and the N′ atom of the hydrazide. The crystal structure is stabilized by inter­molecular N—H⋯O hydrogen bonds, forming one-dimensional chains running parallel to the a axis

Essential oil from Chenopodium ambrosioides L. induces mitochondrial-mediated pathway and endoplasmic reticulum stress-related apoptosis in human liver cancer SMMC-7721 cells

Purpose: To evaluate the cytotoxic effect of essential oil derived from Chenopodium ambrosioides L. in Sichuan Province on human liver cancer SMMC-7721 cells, as well as its possible molecular mechanisms.Methods: Cytotoxicity was characterized by MTT assay and transmission electron microscopy (TEM) of SMMC-7721 cells ultrastructure. The apoptotic effect of the essential oil was evaluated by changes in mitochondrial membrane potential and Western blot assay.Results: MTT assay data indicate that the essential oil was cytotoxic to SMMC-7721 cells, while TEN revealed that there were vacuoles and nucleus fragmentation in the SMMC-7721 cell cytosol, cell swelling, and a large amount of leakage. Mitochondrial membrane potential assay and Western Blot data indicate that the essential oil induced cell apoptosis.Conclusion: The essential oil of Chenopodium ambrosioides L. in Sichuan Province seems to induce apoptosis of human liver cancer SMMC-7721 cells via the mitochondrial-mediated pathway and endoplasmic reticulum stress. Thus, this plant requires further investigation as a potential source of ananti-liver cancer drug. Keywords: Chenopodium ambrosioides Essential oil; Anti-tumor activity, Liver cancer Apoptosis, SMMC-7721 cell

Response of temperate forest ecosystem services to rainfall: A case study in the forest nature reserves of northern China

In the context of global climate change, temperate forests in climate-sensitive areas are inevitably affected. To deepen the understanding of the impact on precipitation changes into the relationship between key ecosystem services (ESs), this study selected net primary productivity (NPP), soil conservation (SC) and water yield (WY) of temperate forest in northern China as objects, and the Spearman correlation test and redundancy analysis were applied to analyze the response of ESs relationship to precipitation gradient. The results show that precipitation is the meteorological factor with the greatest impact (contribution 21.2%, p<0.01) on ESs and their relationships in temperate forests. The 600-700 mm precipitation gradient is the key turning point in the change of ESs relationship of WY with NPP and SC. This indicates that attention should be paid to the spatial variation of the 600-700 mm precipitation region in the future warm-wet in northern China, which should be used as a dividing line of forest management and policy development. Based on the results, future restoration projects in northern temperate forest should focus on (1) in areas with less than 600-700 mm of precipitation, attention should be paid to the selection of tree species for afforestation to maintain regional water balance; (2) in areas with more than 700 mm of precipitation, soil and water conservation projects need to be planned, especially in mountainous area. The research can not only support the management of temperate forest ecosystems in northern China, but also provide reference to other forest ecosystems to cope with climate change

An efficient and rapid method to detect and verify natural antisense transcripts of animal genes

AbstractHigh-throughput sequencing has identified a large number of sense-antisense transcriptional pairs, which indicates that these genes were transcribed from both directions. Recent reports have demonstrated that many antisense RNAs, especially lncRNA (long non-coding RNA), can interact with the sense RNA by forming an RNA duplex. Many methods, such as RNA-sequencing, Northern blotting, RNase protection assays and strand-specific PCR, can be used to detect the antisense transcript and gene transcriptional orientation. However, the applications of these methods have been constrained, to some extent, because of the high cost, difficult operation or inaccuracy, especially regarding the analysis of substantial amounts of data. Thus, we developed an easy method to detect and validate these complicated RNAs. We primarily took advantage of the strand specificity of RT-PCR and the single-strand specificity of S1 endonuclease to analyze sense and antisense transcripts. Four known genes, including mouse β-actin and Tsix (Xist antisense RNA), chicken LXN (latexin) and GFM1 (G elongation factor, mitochondrial 1), were used to establish the method. These four genes were well studied and transcribed from positive strand, negative strand or both strands of DNA, respectively, which represented all possible cases. The results indicated that the method can easily distinguish sense, antisense and sense-antisense transcriptional pairs. In addition, it can be used to verify the results of high-throughput sequencing, as well as to analyze the regulatory mechanisms between RNAs. This method can improve the accuracy of detection and can be mainly used in analyzing single gene and was low cost

Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII a in epileptic hippocampal neurons

Purpose: To investigate the mechanism of the anti-epileptic effect of Ganoderma lucidum polysaccharides (GLP), the changes of intracellular calcium and CaMK II a expression in a model of epileptic neurons were investigated. Method: Primary hippocampal neurons were divided into: 1) Control group, neurons were cultured with Neurobasal medium, for 3 hours; 2) Model group I: neurons were incubated with Mg2+ free medium for 3 hours; 3) Model group II: neurons were incubated with Mg2+ free medium for 3 hours then cultured with the normal medium for a further 3 hours; 4) GLP group I: neurons were incubated with Mg2+ free medium containing GLP (0.375 mg/ml) for 3 hours; 5) GLP group II: neurons were incubated with Mg2+ free medium for 3 hours then cultured with a normal culture medium containing GLP for a further 3 hours. The CaMK II a protein expression was assessed by Western-blot. Ca2+ turnover in neurons was assessed using Fluo-3/AM which was added into the replacement medium and Ca2+ turnover was observed under a laser scanning confocal microscope. Results: The CaMK II a expression in the model groups was less than in the control groups, however, in the GLP groups, it was higher than that observed in the model group. Ca2+ fluorescence intensity in GLP group I was significantly lower than that in model group I after 30 seconds, while in GLP group II, it was reduced significantly compared to model group II after 5 minutes. Conclusion: GLP may inhibit calcium overload and promote CaMK II a expression to protect epileptic neuron

Acute Stress Facilitates LTD Induction at Glutamatergic Synapses in the Hippocampal CA1 Region by Activating μ-Opioid Receptors on GABAergic Neurons

Acute stress impairs recall memory through the facilitation of long-term depression (LTD) of hippocampal synaptic transmission. The endogenous opioid system (EOS) plays essential roles in stress-related emotional and physiological responses. Specifically, behavioral studies have shown that the impairment of memory retrieval induced by stressful events involves the activation of opioid receptors. However, it is unclear whether signaling mediated by μ-opioid receptors (μRs), one of the three major opioid receptors, participates in acute stress-related hippocampal LTD facilitation. Here, we examined the effects of a single elevated platform (EP) stress exposure on excitatory synaptic transmission and plasticity at the Schaffer collateral-commissural (SC) to CA1 synapses by recording electrically evoked field excitatory postsynaptic potentials and population spikes of hippocampal pyramidal neurons in anesthetized adult mice. EP stress exposure attenuated GABAergic feedforward and feedback inhibition of CA1 pyramidal neurons and facilitated low-frequency stimulation (LFS)-induced long-term depression (LTD) at SC-CA1 glutamatergic synapses. These effects were reproduced by exogenously activating μRs in unstressed mice. The specific deletion of μRs on GABAergic neurons (μRGABA) not only prevented the EP stress-induced memory impairment but also reversed the EP stress-induced attenuation of GABAergic inhibition and facilitation of LFS-LTD. Our results suggest that acute stress endogenously activates μRGABA to attenuate hippocampal GABAergic signaling, thereby facilitating LTD induction at excitatory synapses and eliciting memory impairments

A simulation study on the measurement of D0-D0bar mixing parameter y at BES-III

We established a method on measuring the \dzdzb mixing parameter $y$ for BESIII experiment at the BEPCII $e^+e^-$ collider. In this method, the doubly tagged $\psi(3770) \to D^0 \overline{D^0}$ events, with one $D$ decays to CP-eigenstates and the other $D$ decays semileptonically, are used to reconstruct the signals. Since this analysis requires good $e/\pi$ separation, a likelihood approach, which combines the $dE/dx$, time of flight and the electromagnetic shower detectors information, is used for particle identification. We estimate the sensitivity of the measurement of $y$ to be 0.007 based on a $20fb^{-1}$ fully simulated MC sample.Comment: 6 pages, 7 figure