Comparative transcriptome analysis reveals the adaptive mechanisms of halophyte Suaeda dendroides encountering high saline environment

Suaeda dendroides, a succulent euhalophyte of the Chenopodiaceae family, intermittently spread around northern Xinjiang, China, has the ability to grow and develop in saline and alkali environments. The objective of this study was therefore to investigate the underlying molecular mechanisms of S. dendroides response to high salt conditions. 27 sequencing libraries prepared from low salt (200 mM NaCl) and high salt (800 mM NaCl) treated plants at 5 different stages were sequenced using Illumina Hiseq 2000. A total of 133,107 unigenes were obtained, of which 4,758 were DEGs. The number of DEGs in the high salt group (3,189) was more than the low salt treatment group (733) compared with the control. GO and KEGG analysis of the DEGs at different time points of the high salt treatment group showed that the genes related to cell wall biosynthesis and modification, plant hormone signal transduction, ion homeostasis, organic osmolyte accumulation, and reactive oxygen species (ROS) detoxification were significantly expressed, which indicated that these could be the main mechanisms of S. dendroides acclimate to high salt stress. The study provides a new perspective for understanding the molecular mechanisms of halophytes adapting to high salinity. It also provides a basis for future investigations of key salt-responsive genes in S. dendroides

The impact of community-based pulmonary rehabilitation on the health and lives of migrant workers with pneumoconiosis in China: a qualitative study exploring patient experience

# Background Globally, China has the highest prevalence and incidence of pneumoconiosis, which mainly occurs among migrant workers employed in dusty work environments. Pulmonary rehabilitation (PR) is recommended as the most effective therapeutic strategy in managing chronic obstructive pulmonary disease, and recent quantitative studies have validated PR's effect on the health outcomes of patients with pneumoconiosis. However, qualitative evidence regarding PR's impact on pneumoconiosis patients' health and lives is lacking in the literature. Therefore, this study aimed to address this knowledge gap by exploring the experience of pneumoconiosis patients in community-based PR (CBPR). # Methods A qualitative study based on the phenomenological perspective was conducted. The convenience　sampling method was used to recruit pneumoconiosis patients attending a CBPR program supported by a patient advocacy non-governmental organisation. Web-based semi-structured interviews were conducted using a self-made interview guide. Thematic analysis was performed to analyse the interview data. This study was conducted after gaining ethical approval and informed consent from all participants. # Results Fifteen migrant workers with pneumoconiosis patients aged 49 to 71 years old (median: 54 years old) participated in this study. Four themes were identified from the interview data: triggering of a difficult life, reconstruction of life, sense of empowerment, and gaps in PR. Pneumoconiosis severely impaired participants' well-being, and it triggered heavy financial strain and care burden in their families. The CBPR program allowed them to restore their physical and psychosocial health, and they achieved reconstructing their lives by leading a PR-centred life. Participating in the CBPR program, pneumoconiosis patients recognised multiple supports and were empowered with enhanced disease-coping abilities and strengthened hope to survive. Overall, participants reflected on their positive experience in the CBPR program, despite their unmet needs and existing barriers in PR. # Conclusions The CBPR program integrating multiple supports empowered the vulnerable migrant workers with pneumoconiosis and facilitated their health transition and life reconstruction experience. To optimise their PR experience and improve their QoL, health service addressing their unmet needs and barriers in PR is needed. PR for pneumoconiosis patients with different sociodemographic characteristics and family-oriented management of pneumoconiosis should be explored in future research

Giant perivascular spaces: two case reports

Giant perivascular spaces or Virchow-Robin spaces (VRSs) are uniquely inherent developmental malformation and are generally lined by ependymal or leptomeningeal cells. The cerebral hemispheres with VRSs present multiple cysts of curvilinear, round, oval, or layered configuration, which have the same signal intensity as the cerebrospinal fluid (CSF) and represent extremely dilated VRSs. The cortex became extremely thin with a well-defined margin. Herein, we report two cases of true giant perivascular spaces and present a review of pertinent literature. A patient has multiple cysts in the unilateral and bilateral and has polycystic liver. The clinical presentation, image logical features, and diagnosis are discussed

Spatiotemporal Variation Characteristics of Groundwater Storage and Its Driving Factors and Ecological Effects in Tibetan Plateau

Known as the “Asian Water Tower”, the Tibetan Plateau (TP) is a rich water resource and serves an important ecological function. Climate change may cause changes to the water cycle, and these changes may affect the alpine vegetation growth. However, the variation characteristics of groundwater storage (GWS) and its driving factors and associated ecological effects in the TP are poorly understood. In this study, terrestrial water storage changes retrieved by GRACE (Gravity Recovery and Climate Experiment) were combined with GLDAS (Global Land Data Assimilation System) to estimate the GWS changes in the TP. The temporal and spatial variation characteristics of GWS were identified using linear regression and the modified Mann–Kendall (MMK) test, respectively. The analyses showed that the GWS of the TP decreased at an average rate of −0.89 mm/a from January 2003 to December 2021, but since January 2016, it gradually recovered at a rate of 1.47 mm/a. This shows that the GWS in the eastern and northern parts of the TP is decreasing, while the GWS in the western and southern parts is increasing. The influence of climate change on GWS in time and space was determined using the correlation analysis method. Decreased precipitation and permafrost degradation caused by increasing temperatures will lead to a decrease in GWS. On the other hand, rising temperatures may result in an increase in GWS in regions where glaciers are distributed. In this study, the ecological effects were represented by the relationship between GWS and vegetation change. A decline in GWS means that the vegetation will not receive enough water, leading to a decrease in the NDVI and the eventual degradation of grassland to sand, desert, or other kinds of unused land on the TP. On the other hand, an increase in GWS would promote vegetation restoration. The results of this study offer a new opportunity to reveal the groundwater changes in a cryosphere region and to assess the impact of changes in hydrological conditions on ecology.</p

The Greenhouse Gas Emission from Portland Cement Concrete Pavement Construction in China.

This study proposes an inventory analysis method to evaluate the greenhouse gas (GHG) emissions from Portland cement concrete pavement construction, based on a case project in the west of China. The concrete pavement construction process was divided into three phases, namely raw material production, concrete manufacture and pavement onsite construction. The GHG emissions of the three phases are analyzed by a life cycle inventory method. The CO₂e is used to indicate the GHG emissions. The results show that for 1 km Portland cement concrete pavement construction, the total CO₂e is 8215.31 tons. Based on the evaluation results, the CO₂e of the raw material production phase is 7617.27 tons, accounting for 92.7% of the total GHG emissions; the CO₂e of the concrete manufacture phase is 598,033.10 kg, accounting for 7.2% of the total GHG emissions. Lastly, the CO₂e of the pavement onsite construction phase is 8396.59 kg, accounting for only 0.1% of the total GHG emissions. The main greenhouse gas is CO₂ in each phase, which accounts for more than 98% of total emissions. N₂O and CH₄ emissions are relatively insignificant

Denoising Analysis of Partial Discharge Acoustic Signal Based on SVMD-PCA

Partial discharge (PD) acoustic signal detection is one of the effective means to assess the insulation status of power transformers. In actual monitoring, white noise is likely to cause strong interference to the partial discharge acoustic signal of the transformer, which seriously affects the discharge fault identification and monitoring results. In order to suppress the interference of white noise in partial discharge detection, this paper proposes an adaptive partial discharge based on the combination of variational mode decomposition (VMD) and principal component analysis (PCA) based on improved Spearman correlation coefficient. The white noise suppression method is analyzed for the separation and denoising of partial discharge acoustic signals in the environment of −10 ∼ 10 dB. Firstly, the Spearman correlation coefficient is used to determine the optimal number of decomposing modes of VMD. Then the decomposed modal components are adaptively reduced and reconstructed by principal component analysis to remove redundant clutter interference and reduce the influence of human error. Finally, through the simulation signal and actual discharge pulse acoustic signal are tested for denoising. The results show that SVMD-PCA can suppress the interference of white noise in partial discharge acoustic signals and extract clean discharge pulse signal characteristics, the method has enhanced anti-noise performance and can effectively suppress white noise interference

Implementation strategies to promote linkage to care for key populations after HIV self-testing: a scoping review

# Background In human immunodeficiency virus (HIV) care, key populations are sex workers, people who inject drugs, prisoners, transgender people, and men who have sex with men (MSM), who are at high risk and burden of HIV infection but face barriers to HIV prevention, treatment, and health services. HIV self-testing (HIVST) is effective in scaling up HIV testing uptake among key populations due to its cost-effective, confidential, and convenient feature. However, lacking linkage to care support remains a key challenge. This scoping synthesised the global evidence regarding implementing strategies to promote linkage to care for key populations after HIVST. # Methods This scoping review was conducted according to the Joanna Briggs Institute Manual for Evidence Synthesis. A data search was conducted on September 1, 2021. The searched databases were PubMed, Embase, EBSCOhost, Cochrane Library, Wan Fang Data (Chinese), China National Knowledge Infrastructure (Chinese), and Japan Medical Abstracts Society (Japanese). # Results Twenty studies from 2011 records met the eligibility criteria and were included for review. The study populations were MSM (9), mixed high-risk populations (7), female sex workers (3), and transgender youth (1). Implementation strategies to promote linkage to care for key populations after HIVST were classified into seven categories: 1) HIVST kits with linkage-to-care information, 2) web-based or mobile app-guided HIVST, 3) remote HIVST counselling, 4) intensive follow-up, 5) HIVST promotion through social media and key opinion leaders, 6) community engagement in HIVST and 7) financial incentive. The digital-supported intervention mainly targeted MSM in high-income settings, and the community-based approach and financial incentive strategies were mainly implemented in low and middle-income settings. Comparison of linkage outcomes between studies is problematic due to mixed interpretation and measurement of linkage to care after HIVST. # Conclusions Implementation strategies to promote linkage to care for key populations are diverse and should be incorporated in different settings and backgrounds. Innovative digital-supported HIVST research for female sex workers and high-risk populations in low and middle-income settings is warranted. A standardised definition of linkage to care following HIVST and a unified measurement of linkage outcomes should be developed and applied in future research