Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow

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A high throughput approach for discovery of catalytic nucleic acids

Poster Presentation: no. P4Enzymes are biologic polymers. Catalytic nucleic acids are extremely useful and hence there is constant need for improving and discovering of catalytic molecules for industrial, medical and biotechnological applications. In vitro evolution has established that single-stranded nucleic acids can display substrate dependent catalysis of specific biochemical reactions. These single stranded oligonucleotides are called aptamers. Mass production and qualitative detection of single-stranded deoxyribonucleic acids are essential for each round of successful in vitro evolutionary pathways for high affinity binding or catalytic aptamers. In this work we optimized the asymmetric polymerase chain reaction protocol for mass production and subsequently developed a new assay system for detection and quantification of single-stranded deoxyribonucleic acid on the native TBE gel. Further enzyme assay of asymmetric polymerase chain reaction product reflect the quality of single-stranded nucleic acids present in the bulk reaction. We would further translate the ePCR approach into the most powerful ultrahigh-throughput inexpensive droplet-based microfluidics system for discovery of new catalytic nucleic acid variants having stronger catalytic activity. Isolate variants can further be used in diagnostic assay upon modification of nucleotides for resistance to enzymatic and chemical degradation

Ultrafast label-free multi-parametric cellular analysis by interferometric time-stretch microscopy

Session: Best Student Paper Award Competition (ATh1I): paper no. ATh1I.7We present label-free multi-parametric single-cell analysis by ultrafast quantitative phase-contrast imaging flow cytometry (a flow speed up to 8 m/s) based on interferometric time-stretch microscopy, operating at an ultrahigh frame rate of 26 MHz

Ultrafast high-contrast microfluidic cellular imaging by asymmetric-detection time-stretch optical microscopy (ATOM)

FW6A.7.pdfWe demonstrate asymmetric-detection time-stretch optical microscopy which delivers high-contrast (simultaneous enhanced phase-gradient and absorption contrasts) microfluidic imaging with subcellular resolution and in-line optical image amplification (20dB), at a record imaging flow speed of 10 m/s. © 2013 OSAlink_to_OA_fulltex