34 research outputs found
A framework for the detection of de novo mutations in family-based sequencing data
Francioli LC, Cretu-Stancu M, Garimella KV, et al. A framework for the detection of de novo mutations in family-based sequencing data. European Journal of Human Genetics. 2016;25(2):227-233
NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation
How neuronal proteomes self-organize is poorly understood because of their inherent molecular and cellular complexity. Here, focusing on mammalian synapses we use blue-native PAGE and ‘gene-tagging’ of GluN1 to report the first biochemical purification of endogenous
NMDA receptors (NMDARs) directly from adult mouse brain. We show that NMDARs partition between two discrete populations of receptor complexes and B1.5MDa supercomplexes.
We tested the assembly mechanism with six mouse mutants, which
indicates a tripartite requirement of GluN2B, PSD93 and PSD95 gate the incorporation of receptors into B1.5MDa supercomplexes, independent of either canonical PDZ-ligands or GluN2A. Supporting the essential role of GluN2B, quantitative gene-tagging revealed a fourfold molar excess of GluN2B over GluN2A in adult forebrain. NMDAR supercomplexes
are assembled late in postnatal development and triggered by synapse maturation involving epigenetic and activity-dependent mechanisms. Finally, screening the quaternary organization of 60 native proteins identified numerous discrete supercomplexes that populate the
mammalian synapse
Asymmetrical barcode adapter-assisted recovery of duplicate reads and error correction strategy to detect rare mutations in circulating tumor DNA
Reasons for Reprogramming Dual Chamber Pacemakers to VVI Mode: A Retrospective Review Using a Computer Database
Contemporary practice and short-term outcomes after liver resections in a complete national cohort
Neutrophils promote inflammatory angiogenesis via release of preformed VEGF in an in vivo corneal model
10.1007/s00441-009-0908-5Cell and Tissue Research3392437-44