5 research outputs found
Structure of the cohesin loader Scc2
The functions of cohesin are central to genome integrity, chromosome organization and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2âScc4 complex; however, little is known about how it stimulates the cohesion-loading activity. Here we determine the large âhook' structure of Scc2 responsible for catalysing cohesin loading. We identify key Scc2 surfaces that are crucial for cohesin loading in vivo. With the aid of previously determined structures and homology modelling, we derive a pseudo-atomic structure of the full-length Scc2âScc4 complex. Finally, using recombinantly purified Scc2âScc4 and cohesin, we performed crosslinking mass spectrometry and interaction assays that suggest Scc2âScc4 uses its modular structure to make multiple contacts with cohesin