A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Food-grade sugar can promote differentiation in melon (Cucumis melo L.) tissue culture

The objective of the present study was to investigate the origin of discrepancy between experimental results in in vitro culture of Turkish melon (Cucumis melo L.) cultivars, conducted by the same individual using the same protocol and same seed batches in two different laboratories. The difference in the sucrose source was found to be the major reason for the deviation in results between the two laboratories. The percentage of regenerating explants and the number of bud-like protuberances and/or shoots were significantly greater when a food-grade Turkish sucrose was used in the medium compared with analytical-grade sucrose. Media formulated with the food-grade sucrose regenerated 37 and 67 % more explants and bud-like protuberances and/or shoots per explant, respectively, than media containing analytical-grade sucrose. No meaningful differences were found in added elements or anions between the sucrose sources or by liquid chromatography/mass spectroscopy. The only significant chemical difference observed between the sucrose samples was the presence of melanoidins (Maillard reaction products) in the food-grade sucrose. The melanoidins were of high molecular weight (>3,000 Da determined by ultrafiltration), with characteristic ultraviolet–visible spectra and in vitro antioxidant activity. Melanoidin-containing sucrose can be differentiated by color and spectroscopy

Reactivity and Environmental Factors

Hydrated mineral trioxide aggregate (MTA) is composed of calcium silicate hydrate, calcium hydroxide, ettringite, monosulphate and bismuth oxide together with unreacted tricalcium and dicalcium silicate. The presence of calcium hydroxide, which is usually leached out of the material, renders MTA reactive and thus susceptible to changes as a result of the environment in which it is placed. The specific alterations to the material will vary depending on its use and thus its relationship with other materials, tissues and fluids