Simultaneous determination of aliskiren, amlodipine and hydrochlorothiazide in spiked human plasma and urine by high performance liquid chromatography

A reversed-phase (RP) liquid chromatographic technique for the simultaneous determination of aliskiren (ALS), amlodipine (AMD) and hydrochlorothiazide (HCT) in spiked human plasma and urine is presented. The method employs a gradient elution using 10 mM orthophosphoric acid containing 0.1% triethylamine (pH 2.5, v/v) and acetonitrile and an RP-C-18 column (4.6 mm x 250 mm, 5 mu m, Phenomenex) at 1 mL/min of flow rate, with a UV photo diode array detector at 271 nm. The linear ranges were 0.01-10 mu g/mL in plasma and 0.05-10 mu g/mL in urine for both ALS and AMD. The linearty of HCT was in the range of 0.0125-2.5 mu g/mL in plasma and urine. Correlation coefficients (r (2)) were higher than 0.9983 for all of the analytes, indicating good linear relationship. The method validation was performed with respect to linearity, recovery, accuracy, precision and stability. The developed method could be applied in the routine clinical analysis

Adoption of Internet of Things in Healthcare Organizations

The Internet of things (IoT) is a paradigm that integrates different types of devices which can operate in any environment with their sensing, communicating, and processing capability. This domain has been a revolutionary changing of healthcare operations by providing a new architecture for devices and applications through IP-based connection capability. Furthermore, IoT is a generic platform to exchange information between mobile health devices and other ubiquitous technologies. Although there have been several studies that focus on IoT applications in healthcare, adoption of IoT in healthcare remains largely unknown. The goal of this study is (i) to close the gap in literature by addressing IoT adoption in healthcare; (ii) to help decision-makers to understand the main variables which affect adoption decision among different users in terms of top level managers, healthcare professionals, technical staff, and patients; and (iii) to present recommendations for healthcare managers to improve the adoption process of IoT devices in their organizations. We believe that this study would provide a better understanding of main barriers and facilitators regarding user perspectives during organizational IoT transformation in healthcare