Low genetic diversity and absence of population differentiation of hilsa (Tenualosa ilisha) revealed by mitochondrial DNA cytochrome b region in Ganga and Hooghly rivers

We investigated the mtDNA cytochrome b based genetic structure of anadromous clupeid hilsa, Tenualosa ilisha, from the rivers Ganga and Hooghly. Six different haplotypes were observed, in sample size of 240, with a single dominant haplotype present in both rivers. Analysis of molecular variance (AMOVA) of Ganga and Hooghly populations does not suggest existence of population structuring in hilsa. AMOVA conducted on the whole population from Ganga and Hooghly suggested existence of a single population, migrating to Ganga and Hooghly rivers through the estuaries for spawning and breeding.Keywords: Hilsa, Tenualosa ilisha, Ganga, Hooghly, Bay of Bengal, West Bengal, India, mtDNA cytochrome bAfrican Journal of Biotechnology Vol. 12(22), pp. 3383-338

High levels of genetic variability and differentiation in hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) populations revealed by PCR-RFLP analysis of the mitochondrial DNA D-loop region

The hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) is an important anadromous clupeid species from the Western division of the Indo-Pacific region. It constitutes the largest single fishable species in Bangladesh. Information on genetic variability and population structure is very important for both management and conservation purposes. Past reports on the population structure of T. ilisha involving morphometric, allozyme and RAPD analyses are contradictory. We examined genetic variability and divergence in two riverine (the Jamuna and the Meghna), two estuarine (Kuakata and Sundarbans) and one marine (Cox's Bazar) populations of T. ilisha by applying PCR-RFLP analysis of the mtDNA D-loop region. The amplified PCR products were restricted with four restriction enzymes namely, XbaI, EcoRI, EcoRV, and HaeIII. High levels of haplotype and gene diversity within and significant differentiations among, populations of T. ilisha were observed in this study. Significant FST values indicated differentiation among the river, estuary and marine populations. The UPGMA dendrogram based on genetic distance resulted in two major clusters, although, these were subsequently divided into three, corresponding to the riverine, estuarine and marine populations. The study underlines the usefulness of RFLP of mtDNA D-loop region as molecular markers, and detected at least two differentiated populations of T. ilisha in Bangladesh waters

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Not AvailableThe lockdowns imposed to contain the COVID-19 spread had an indisputably cascading effect on the aquaculture industry across the globe. It has adversely impacted the Indian fisheries sector through changing purchaser demand, supply chain disruptions, trade restrictions, and also the livelihood of millions dependent on it. However, the consequential reduced fishing will also likely help in replenishment of the aquatic biodiversity and provide the balance needed in the long-term sustenance of the fisheries sector. The technological and policy interventions in pre- and post-harvest fisheries operations are anticipated to reduce some of these negative impacts in similar lockdown scenarios.Not Availabl

NIASM Annual Report 2010-11

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Not AvailableHeat shock proteins (Hsp) are known to play major role in protection of cells from thermal stress. Nucleotide polymorphisms within the promoter of Hsp affect degree of expression and inducibility of Hsp mRNA. The present study aimed to investigate the effect of polymorphism within promoter region on the cellular expression of Hsp70.1 mRNA and association of identified polymorphisms with the physiological parameters during summer stress and milk production traits in dairy cattle. Two hundred Frieswal cows were genotyped using double PCR-RFLP to identify deletion of cytosine within the Hsp70.1 promoter AP2 box at base position 895. Homozygous wild type genotypes (CC) were found in lower frequency (39.29, n=78) than heterozygous cytosine deletion mutant genotypes (C-) (60.71, n=122). In the observed physiological parameters (rectal temperature, respiration rate and heat tolerance coefficient), cows that were homozygous wild types had better significant (P<0.05) summer tolerance than the heterozygous deletion genotypes. Cytosine deletion mutation in the promoter region negatively affected (P<0.01) the expression of Hsp70.1 mRNA in peripheral bovine mononuclear cells (PBMC) subjected to in vitro heat stress. Further association of observed polymorphism with the milk production traits was significant as the heterozygous cytosine deletion cows had lower total milk yield, peak yield, yield at 300 days, protein% (P<0.01) and fat% (P<0.05) than the native wild type promoter cows. The results from the present study suggest that the promoter region of bovine hsp70.1 gene is polymorphic and may be useful in selection of dairy cows for relatively better thermotolerance and higher milk production.Not Availabl