Utilização de lactose na dieta de leitões desmamados aos 21 dias de idade.

bitstream/item/58417/1/CUsersPiazzonDocuments228.pd

Recently Integrated \u3cem\u3eAlu\u3c/em\u3e Elements in Capuchin Monkeys: A Resource for \u3cem\u3eCebus\u3c/em\u3e/\u3cem\u3eSapajus\u3c/em\u3e Genomics

Capuchins are platyrrhines (monkeys found in the Americas) within the Cebidae fam-ily. For most of their taxonomic history, the two main morphological types of capuchins, gracile (untufted) and robust (tufted), were assigned to a single genus, Cebus. Further, all tufted capuchins were assigned to a single species, Cebus apella, despite broad geographic ranges spanning Central and northern South America. In 2012, tufted capuchins were assigned to their genus, Sapajus, with eight currently recognized species and five Cebus species, although these numbers are still under debate. Alu retrotransposons are a class of mobile element insertion (MEI) widely used to study primate phy-logenetics. However, Alu elements have rarely been used to study capuchins. Recent genome-level assemblies for capuchins (Cebus imitator; [Cebus_imitator_1.0] and Sapajus apella [GSC_monkey_1.0]) facilitated large scale ascertainment of young lineage-specific Alu insertions. There are 1607 capuchin specific and 678 Sapajus specific Alu insertions along with candidate oligonucleotides for locus-specific PCR assays for many elements. PCR analyses identified 104 genus level and 51 species level Alu insertion polymorphisms. The Alu datasets reported in this study provide a valuable resource that will assist in the classification of archival samples lacking phenotypic data and for the study of capuchin phylogenetic relationships

Caracterização do perfil sorológico de nulíparas suínas e da progênie frente ao parvovírus suíno.

Projeto: 03.09.00.046

The Meta-Position of Phe4 in Leu-Enkephalin Regulates Potency, Selectivity, Functional Activity, and Signaling Bias at the Delta and Mu Opioid Receptors

This work is licensed under a Creative Commons Attribution 4.0 International License.As tool compounds to study cardiac ischemia, the endogenous δ-opioid receptors (δOR) agonist Leu5-enkephalin and the more metabolically stable synthetic peptide (d-Ala2, d-Leu5)-enkephalin are frequently employed. However, both peptides have similar pharmacological profiles that restrict detailed investigation of the cellular mechanism of the δOR’s protective role during ischemic events. Thus, a need remains for δOR peptides with improved selectivity and unique signaling properties for investigating the specific roles for δOR signaling in cardiac ischemia. To this end, we explored substitution at the Phe4 position of Leu5-enkephalin for its ability to modulate receptor function and selectivity. Peptides were assessed for their affinity to bind to δORs and µ-opioid receptors (µORs) and potency to inhibit cAMP signaling and to recruit β-arrestin 2. Additionally, peptide stability was measured in rat plasma. Substitution of the meta-position of Phe4 of Leu5-enkephalin provided high-affinity ligands with varying levels of selectivity and bias at both the δOR and µOR and improved peptide stability, while substitution with picoline derivatives produced lower-affinity ligands with G protein biases at both receptors. Overall, these favorable substitutions at the meta-position of Phe4 may be combined with other modifications to Leu5-enkephalin to deliver improved agonists with finely tuned potency, selectivity, bias and drug-like properties

Coeficientes técnicos para o cálculo do custo de produção de suínos, 2012.

bitstream/item/78973/1/Comunicado-506.pdfProjeto/Plano de Ação: 04.08.08.017

Efeitos dos fosfatos de tapira e nonocálcico no desempenho produtivo e reprodutivo de suínos. I - gestação e lactação.

Com o objetivo de comparar fontes de fósforo(Fosfato Bicálcico, Tapira e Monocálcico) para porcas durante a reprodução (gestação e lactação), foi conduzido um experimento no CNPSA envolvendo 60 marrãs Landrace x Large White com peso médio inicial de 135,60 kg

Estudo da ocorrência de circovírus suíno tipo 2 (PCV2) em suínos vivos ou em materiais com suspeita clínica de circovirose suína enviados para diagnóstico.

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Novel putative candidate genes associated with umbilical hernia in pigs.

Abstract: Umbilical hernia is one of the most frequent anatomical defects in pigs in which abdominal contents protrude through the umbilical ring. This condition is considered to have a multifactorial basis in which environmental, infectious and genetic factors play a role. However, a better understanding about the genetic components involved in the umbilical hernia development has not yet been achieved. Although a few studies have mapped QTL for umbilical hernia, just a few candidate genes were reported. Therefore, the aim of this study was to identify genomic regions related to the development of umbilical hernias in pigs and search for potential candidate genes. A GWAS was performed with 92 cases and 233 control crossbred pigs. Five SNPs were associated with umbilical hernia: on SSC4/SSC6/SSC13 and one with unknown position. Candidate genes TBX15 and WARS2 were identified close to the SNP on SSC4. Another two candidate genes were located near the SNP associated with umbilical hernia on SSC13 (LIPI and RBM11). Further validation of these genes should be performed to improve the knowledge about umbilical hernia development in order to improve pig welfare and production by eliminating susceptible animals through genetic selection Resumo: A hérnia umbilical é um dos defeitos anatômicos mais freqüentes em suínos nos quais o conteúdo abdominal se projeta através do anel umbilical. Considera-se que esta condição tem uma base multifatorial na qual fatores ambientais, infecciosos e genéticos desempenham um papel. Entretanto, um melhor entendimento sobre os componentes genéticos envolvidos no desenvolvimento da hérnia umbilical ainda não foi alcançado. Embora alguns estudos tenham mapeado o QTL para a hérnia umbilical, apenas alguns genes candidatos foram relatados. Portanto, o objetivo deste estudo foi identificar regiões genômicas relacionadas ao desenvolvimento de hérnias umbilicais em porcos e buscar potenciais genes candidatos. Um GWAS foi realizado com 92 casos e 233 porcos cruzados de controle. Cinco SNPs foram associados com hérnia umbilical: em SSC4 / SSC6 / SSC13 e um com posição desconhecida. Os genes candidatos TBX15 e WARS2 foram identificados próximos ao SNP em SSC4. Outros dois genes candidatos foram localizados perto do SNP associado à hérnia umbilical na SSC13 (LIPI e RBM11). A validação adicional desses genes deve ser realizada para melhorar o conhecimento sobre o desenvolvimento de hérnia umbilical, a fim de melhorar o bem-estar ea produção de suínos, eliminando os animais suscetíveis por meio da seleção genétic

Discrimination between oral corticosteroid-treated and oral corticosteroid-non-treated severe asthma patients by an electronic nose platform

Rationale: Some severe asthma patients require oral corticosteroids (OCS) likely due to greater disease severity. Exhaled molecular markers can provide phenotypic information in asthma. Objectives: Determine whether patients on OCS (OCS+) have a different breathprint compared with those who were not on OCS (OCS-); determine the classification accuracy of eNose as compared to FEV1 % pred, % sputum eosinophils, and exhaled nitric oxide (FENO). Methods: This was a cross-sectional analysis of the U-BIOPRED cohort. Severe asthma was defined by IMI-criteria [Bel Thorax 2011]. OCS+ patients had daily OCS. OCS- patients had never had OCS and were on maintenance inhaled fluticasone equivalent >1000 μg/day. Exhaled volatile organic compounds trapped on adsorption tubes were analysed by centralized eNose platform (Owlstone Lonestar, Cyranose 320, Comon Invent, Tor Vergata TEN) including a total of 190 sensors. t test was used for comparing groups and support vector machine with leave-one-out cross-validation as a classifier. Results: 33 OCS+ (age 55±11yr, mean±SD, 52% female, 27% smokers, pre-bronchodilator FEV1 64.1±24% pred) and 40 OCS- severe asthma patients (age 54±15yr, mean±SD, 55% female, 35% smokers, pre-bronchodilator FEV1 61.8±24% pred) were studied. Sensor by sensor analysis showed that 56 sensors provided different mean values (change in sensor resistance or frequency) between groups (P<0.05). Accuracy of classification was as follows: eNose 71% (n=73), FENO 71% (n=70), FEV1 62% (n=73) and sputum eosinophils 59% (n=37). Conclusions: Preliminary results suggest OCS+ and OCS- severe asthma patients can be distinguished by an eNose platform