Comparative Anatomy Of Cotyledons And Eophylls Laminas Of Ten Species Of Caesalpinioideae (fabaceae) [anatomia Comparada Dos Limbos Cotiledonares E Eofilares De Dez Espécies De Caesalpinioideae (fabaceae)]

The anatomy of cotyledons and eophylls laminas of Caesalpinia leiostachya (Benth.) Ducke, Dimorphandra mollis Benth., Peltophorum dubium (Spreng.) Taub., Pterogyne nitens Tul., Schizolobium parahyba (Vell.) Blake (Caesalpinieae), Cassia ferruginea (Schrad.) Schrad. ex DC., Senna multijuga (Rich.) Irwin & Barn. (Cassieae), Bauhinia forficata Link (Cercideae), Copaifera langsdorffii Desf. and Hymenaea stilbocarpa Hayne (Detarieae) was compared. In most species, cotyledons epidermal cells presented straight anticlinal walls and eophylls had sinuous ones. Most of cotyledons were amphistomatic, while eophylls, hypostomatic. Cotyledons had homogeneous mesophylls, and eophylls dorsiventral ones. There are specific variation on the presence and distribution of starch grains, phenolic compounds, lipids, and polysaccharides. Cotyledons and eophylls presented collateral vascular bundles, with fibers and/or parenchyma sheath, in which crystals often occur. We suggest that: i) there is an increase of structural complexity of the eophylls compared to the cotyledons, ii) this phenomenon could be explained by the functions and short life span of the cotyledons.292193207BOELCKE, O., Estudio morfológico de las semillas de Leguminosas, Mimosoideas y Caesalpinioideas de interés agronómico en la Argentina (1946) Darwiniana, 7, pp. 240-321(1992) Regras para análise de sementes, , BRASIL, 2a ed, Agricultura. Departamento Nacional de Produção Vegetal, BrasíliaBUKATSCH, F., Bemerkungen zur doppelfärbung Astrablau-Safranin (1972) Mikrokosmos, 61, p. 255COMPTON, R.H., Investigation of the seedling structure in the Leguminosae (1912) Journal of the Linnean Society of London Botany, 41, pp. 1-122CORNER, E.J.H., The leguminous seed (1951) Phytomorphology, 1, pp. 117-150ESAU, K. 1974. Anatomia das plantas com sementes (B.L. Morretes, trad.). Edgard Blücher, São PauloFAHN, A., (1990) Plant anatomy, , 4th ed. Pergamon Press, OxfordGARWOOD, N.C., Studies in Annonaceae. XX. Morphology and ecology of seedlings, fruits and seeds of selected Panamanian species (1995) Botanische Jahrbuecher fuer Systematik, 117, pp. 1-152GIFFORD, E.M., FOSTER, A.S., (1989) Morphology and evolution of vascular plants, , 3rd ed. W.H. Freeman, New YorkGUNN, C.R., Fruits and seeds of genera in the subfamily Mimosoideae (Fabaceae) (1984) Technical Bulletin, 1681, pp. 1-194JENSEN, W.A., (1962) Botanical histochemistry: Principle and pratice, , W.H. Freeman, San FranciscoJOHANSEN, D.A., (1940) Plant microtechnique, , McGraw-Hill Book, New YorkJUDD, W.S., CAMPBELL, C.S., KELLOGG, E.A., STEVENS, P.F., (1999) Plant Systematics: A phylogenetic approach, , Sinauer Associates, SunderlandKITAJIMA, K., Relationship between photosynthesis and thickness of cotyledons for tropical tree species (1992) Functional Ecology, 6, pp. 582-589KOOIMAN, P., On the occurrence of amyloids in plant seeds (1960) Acta Botanica Neerlandica, 9, pp. 208-219KOTRESHA, K., SEETHARAM, Y.N., Epidermal studies in some species of Bauhinia L. (Caesalpinioideae) (1995) Phytomorphology, 45, pp. 127-137KRAUS, J.E. & ARDUIN, M. 1997. Manual básico de métodos em Morfologia Vegetal. Edur, SeropédicaLARCHER, W. 2000. Ecofisiologia Vegetal (C.H.B.A. Prado, trad.). RiMa Editora, São CarlosLEELAVATHI, P., RAMAYYA, N., PRABHAKAR, M., Foliar stomatal distribution patterns in Leguminosae and their taxonomic significance (1980) Phytomorphology, 30, pp. 195-204LERSTEN, N.R., CURTIS, J.D., Leaf anatomy in Caesalpinia and Hoffmannseggia (Leguminosae, Caesalpinioideae) with emphasis on secretory structures (1994) Plant Systematics and Evolution, 192, pp. 231-255LERSTEN, N.R., CURTIS, J.D., Survey of leaf anatomy, especially secretory structures, of tribe Caesalpinieae (Leguminosae, Caesalpinioideae) (1996) Plant Systematics and Evolution, 200, pp. 21-39LORENZI, H., (1992) Árvores brasileiras: Manual de identificação e cultivo de plantas arbóreas nativas do Brasil. Plantarum, , Nova OdessaMETCALFE, C.R., The leaf: General topography and ontogeny of the tissues (1979) Anatomy of dicotyledons: Systematic anatomy of the leaf and stem, pp. 97-165. , C.R. Metcalfe & L. Chalk, eds, 2nd ed. Claredon Press, Oxford, pMORAES, P.L.R., PAOLI, A.A.S., Epiderme e padrão de venação foliar de espécies de Lauraceae (1999) Acta Botanica Brasilica, 13, pp. 87-97MORRETES, B.L., Contribuição ao estudo da anatomia das folhas de plantas do cerrado II (1966) Boletim da Faculdade de Filosofia, 305, pp. 209-244. , Ciências e Letras da Universidade de São PauloMOTT, K.A., GIBSON, A.C., O'LEARY, J.W., The adaptive significance of amphistomatic leaves (1982) Plant, Cell and Environment, 5, pp. 455-460NOVOTNÁ, J., Anatomical characteristics of the epidermis in some cultivars of Brassica oleracea L. Distribution and structure of stomata in the cotyledons (1976) Biologia Plantarum, 18, pp. 13-18O'BRIEN, T.P., FEDER, N., MCCULLY, M.E., Polychromatic staining of plant cell walls by toluidine blue O (1964) Protoplasma, 59, pp. 368-373OLIVEIRA, D.M.T., Morfo-anatomia do embrião de leguminosas arbóreas nativas (1999) Revista Brasileira de Botânica, 22, pp. 413-427OLIVEIRA, D.M.T., Morfologia de plântulas e plantas jovens de 30 espécies arbóreas de Leguminosae (1999) Acta Botanica Brasilica, 13, pp. 263-269OLIVEIRA, D.M.T., BELTRATI, C.M., Morfologia e desenvolvimento das plântulas de Inga fagifolia e I. urugüensis (1992) Turrialba, 42, pp. 306-313PYYKKÖ, M., Morphology and anatomy of leaves from some woody plants in a humid tropical forest of Venezuelan Guyana (1979) Acta Botanica Fennica, 112, pp. 1-41POLHILL, R.M., RAVEN, P.H., (1981) Advances in legume systematics, (PART 1). , Crown Copyright, KewREDDY, P.K.R., SHAH, G.L., Observations on the cotyledonary and hipocotyledonary stomata and trichomes in some Caesalpiniaceae with a note on their taxonomic significance (1979) Feddes Repertorium, 90, pp. 239-250RICARDI, M., TORRES, F., HERNÁNDEZ, C. & QUINTERO, R. 1977. Morfologia de plantulas de arboles venezolanos. I. Revista Florestal Venezolana 27:15-56SAINT-MARTIN, M., Ontogenie des plantules et phytodermologie chez les Papilionaceae (1984) Gaussenia, 1, pp. 19-44SCOTT, R.C., SMITH, D.L., Cotyledon architecture and anatomy in the Acacieae (Leguminosae: Mimosoideae) (1998) Botanical Journal of the Linnean Society, 128, pp. 15-44SHAH, G.L., GOPAL, B.V., Development of stomata in some Papilionaceae (1969) Canadian Journal of Botany, 47, pp. 387-393SHAH, G.L., GOPAL, B.V., Structure and development of stomata on the vegetative and floral organs in some members of Caesalpiniaceae (1971) Annals of Botany, 35, pp. 745-759SHARMA, G.K., DUNN, D.B., Effect of environment on the cuticular features in Kalanchoe fedschenkoi (1968) Bulletin of the Torrey Botanical Club, 95, pp. 464-473SMITH, D.L., Cotyledons of the Leguminosae (1981) Advances in legume systematics, pp. 927-940. , R.M. Polhill & P.H. Raven, eds, Royal Botanic Gardens, Kew, pSMITH, D.L., Cotyledon anatomy in the Leguminosae (1983) Botanical Journal of the Linnean Society, 86, pp. 325-355SMITH, D.L., SCOTT, R.C., Cotyledon venation patterns in the Leguminosae: Caesalpinioideae (1985) Botanical Journal of the Linnean Society, 90, pp. 73-103SMITH, D.L., SCOTT, R.C., Cotyledon venation patterns in the Leguminosae: Mimosoideae (1995) Botanical Journal of the Linnean Society, 119, pp. 213-243SWAIN, T., Tannins and lignins (1979) Herbivorous: Their interactions with secondary plant metabolites, pp. 657-682. , G.A. Rosenthal & D.H. Janzen, eds, Academic Press, New York. pTHEOBALD, W.L., KRAHULIK, J.L., ROLLINS, R.C., Trichome description and classification (1979) Anatomy of dicotyledons: Systematic anatomy of the leaf and stem, pp. 40-53. , C.R. Metcalfe & L. Chalk, eds, 2 nd ed. Claredon Press, Oxford, pVALDÉS, A.B., MATOS, A.B., Características epidérmico-foliares en táxones cubanos de la tribu Detarieae (Leguminosae: Caesalpinioideae) (1992) Acta Botanica Cubana, 93, pp. 1-6ZINDLER-FRANK, E., Calcium crystals in legumes (1987) Advances in legume systematics, (PART 3), pp. 279-316. , C.H. Stirton, ed, Royal Botanic Gardens, Kew, pWATSON, R.W., The effect of cuticular hardening on the epidermal cells (1942) New Phytologist, 41, pp. 223-229WILKINSON, H.P., The plant surface (mainly leaf) (1979) Anatomy of dicotyledons: Systematic anatomy of the leaf and stem, pp. 97-165. , C.R. Metcalfe & L. Chalk, eds, 2nd ed. Claredon Press, Oxford,

Estudos morfoanatômicos da semente e da plântula de espécies de Anileiras (Indigofera L., Leguminosae) Morpho-anatomical studies of seeds and seedlings of wild indigo, "anileira", Indigofera- Leguminosae

O nome popular "anileira" designa Indigofera anil L., I. suffruticosa Mill. e I. truxillensis Kunth, muito semelhantes quanto à morfologia externa. Este trabalho teve por objetivo avaliar se caracteres da semente e plântula apresentavam valor diagnóstico para este grupo, já que tais caracteres têm sido muito utilizados na Taxonomia de Leguminosae. A superfície e morfoanatomia de sementes e cotilédones foram estudadas por microscopias eletrônica de varredura e de luz, e as etapas do desenvolvimento das plântulas descritas. Embora as plântulas sejam semelhantes, caracteres de sementes (tamanho, forma, ornamentação da superfície, forma do hilo e tamanho do embrião) e cotilédones (forma, organização do parênquima lacunoso na nervura central e distribuição de metabólitos nos tecidos) apresentaram valor diagnóstico para as espécies. I. anil distingue-se de I. suffruticosa por suas sementes maiores e cotilédones com borda acuminada. I. truxillensis caracteriza-se por apresentar sementes cilíndricas e cotilédones reniformes contendo gotas de óleo e alcalóides. Nossos dados, a morfologia externa dos frutos (curvos em I. anil e I. suffruticosa, e retos em I. truxillensis) e, ainda, a anatomia foliar (células parenquimáticas grandes e fenólicas no floema de I. suffruticosa e sua ausência em I. anil) sugerem que I. anil, I. suffruticosa e I. truxillensis não devem ser sinonimizadas.<br>The common name "wild indigo" specifies Indigofera anil L., I. suffruticosa Mill. (legitimate name) and I. truxillensis Kunth (legitimate name) that are very similar due to their external morphology. This work analyzed diagnostic characteristics of seeds and seedlings of these species since such features are widely used in taxonomic approaches within Leguminosae. We studied surface features and morpho-anatomy of seeds and cotyledons with scanning electronic microscopy and light microscopy, and described seedling phases. Although seedlings are similar, seed characteristics (size, shape, surface ornamentation, shape of the hilum and embryo size) and cotyledon characteristics (shape, trichome ornamentation, organization of spongy parenchyma along central veins and metabolite distribution in the tissues) have diagnostic features for the species. I. anil differs from I. suffruticosa in its larger seeds and acuminate-margined cotyledons. I. truxillensis is recognized by its cylindrical seeds and kidney-shaped cotyledons with large oil drops. We assume that the characteristics examined, plus fruit morphology and foliar anatomy suggest that I. anil, I. suffruticosa and I. truxillensis should not be synonymized