Bacillus thuringiensis production using cassava liquid waste (manipueira) as a fermentation substrate.

In developing countries the search for inexpensive substrates to grow useful microorganisms is a desirable way to get new and safe products to protect man against insect pest losses. Bacillus thuringiensis is an entomopathogenic bacteria, which by fermentation produces endo- or exotoxins to be used against Lepidoptera or Diptera, resp. These are insect pests of economic importance in developing countries, mainly in Brazil, where 60% losses in agriculture are caused by this type of pest. Our research group has been working with B. thuringiensis since 1970; and many papers have been published on the processes, culture media composition and available substrates, culture and operational conditions, equipment and so on. Many residues and waste waters were tried forthe fermentation culture medium. This paper reports the possibility of using manipueira, a residual waste water from cassava processing as a substrate to produce B. thuringiensis by fermentation. The culture medium was composed of 75% manipueira & 25% water. Operational conditions considered agitation and aeration rate, as well as initial pH and optimum growing temperature. Microbial growth and sporulation behavior were accomplished by optical density determination, pH variation and sugar consumption. Results are promising

Identification of endophytic bactéria from micropropagated yacon (Smallanthus sonchifolius) crowing under autotrophic and Heterotrophic systems.

Yacon, Smallanthus sonchifolius, an Andean species. is a rich source of dictetíc oligofructans with low glucose content. proteins and phenolic compounds. These constituents have shown efficacy in the prevention of diet-related ehronic diseases, including gastroin-testinal disorders and diabetes |1,2|. Yacon is part of a research program at the National Center for Natural Products Research (NCNPR) and University of Mississippi Field Station to develop new alternative root crops for Mississippi while attempting to im-prove the diet of low incorne families. Yacon can be easily propa-gated by cultings. Virus and nematode infections have been re-ported on plants propagated by cuttings in Brazil. a country that hás adopted Yacon as specialty crop [3|. We have developed two culture systems. autotrophic and heterotrophic, to produce healthy plants. Herem we describe the presence of endophytic bactéria m micropropagated Yacon. In auxin free media, new roots were induced. Overa 15day period. the average root mduction per expiam was 5.45 to 8.75 under autotrophic and heterotrophic cul-tures, respectively. Root lenglh vaned between 3 and 60mrn. The presence of root hairs and lateral roots was noticed only in auto-trophic condilions. These beneficiai bactéria were identified and chemically ctiaracterized. Acknowledgement: This research work was partially supported by the USDA/ARS Cooperative Research Agreement No. 58-6408-2-009. Referentes; |1) Terada S. et ai. (2006] Yakugaku Zasshi 126(8): 665-669. (2| Valentová K. Ulri-chová j. (2003) Biomedical Papers 147: 119-130. [3| Mogor C. et ai, (2003) Acta Horticulturea 597: 311 -313

Resíduo micropulverizado da moagem a seco de milho não afeta biodisponibilidade de zinco em ratos.

Trabalho apresentado no XV Congresso Latinoamericano de Nutricion e XVI Jornadas de la Sociedad Chilena de Nutrición, 2009, Santiago do Chile

Progression of myopathology in Kearns-Sayre syndrome

We report on the progression of myopathology by comparing two biopsies from a patient with a Kearns-Sayre-Syndrome. The first biopsy was taken in 1979 and showed 10% ragged-red fibers. Myopathic changes were slight including internal nuclei and fiber splitting in 10% of the fibers. Electron microscopy revealed typical mitochondrial abnormalities with regard to number and shape. In 1989 a second biopsy was performed for an extended analysis of mitochondrial DNA. This time less than 5% of all fibers were ragged-red. Severe myopathic changes could be detected which so far has rarely been reported in mitochondrial cytopathy

Alternativas ao processo tradicionalmente utilizado na província de nampula (Moçambique) para a produção de suco de caju.

Neste documento, descrevem-se dois processos para o aproveitamento do pedúnculo de caju: produção de suco parcialmente clarificado, com teor baixo de polpa, e suco de caju clarificado, isento de polpa, adaptados das condições tradicionais de produção da cajuína brasileira para a realidade moçambicana, e que permite obter um produto com boas características sensoriais e estabilidade de aproximadamente 60 dias estocados em temperatura ambiente.bitstream/item/55351/1/COT11019.pd

Efeito da restrição animal no período seco sobre o desempenho de bovinos de recria.

bitstream/item/137687/1/PESQ-EM-ANDAMENTO-47.pdfCNPGC

Variação individual de ovos de nematódeos gastrintestinais por grama de fezes (OPG) dentro de um rebanho ovino.

A verminose é um dos principais entraves da produção ovina a nível mundial. O controle dos nematódeos gastrintestinais é realizado quase exclusivamente através da aplicação de anti-helmínticos, no entanto, o crescente problema da resistência dos parasitos estes fármacos (WALLER, 1991) tem limitado os níveis produtivos da ovinocultura.bitstream/item/55661/1/CT32.pd