Dedifferentiation of hematopoietic stem cells into induced pluripotent stem cells

71 p.In 2006,"iPS cells" were introduced for the first time in the field of biology by a group of scientists at Kyoto University led by Shinya Yamanaka. iPS cells which stands for induced pluripotent stem cells has opened a new horizon for researchers in stem cell and related areas especially in cell transplantation therapies. Since then many groups all over the world have started to explore this field and have used different strategies to improve the methods of generating iPS cells.​Master of Scienc

IDENTIFYING AND CHARACTERIZING TUMOR INITIATING AND CIRCULATING TUMOR CELLS IN LUNG CANCER

Ph.DDOCTOR OF PHILOSOPH

Use of UTF1 genetic control elements as iPSC reporter

The reprogramming of adult somatic cells into an embryonic stem cell (ESC) state by various means has opened a new chapter in basic and applied life science. While this technology will create great opportunities for regenerative medicine, the more immediate impact is likely to be found in human disease modeling and drug testing/development. An important aspect in the latter contexts is the ability to reliably monitor the pluripotent stem cell state, in particular with respect to human cell reprogramming using patient-specific somatic cells and high-throughput screens. Undifferentiated transcription factor 1 (UTF1) belongs to the core transcriptional network characterizing pluripotency. UTF1 is involved in ESC-specific chromatin organization, and its expression pattern during cell reprogramming and subsequent differentiation appears to be tightly connected with the pluripotent stem cell state. Here, we capitalized on these features and generated a reliable reporter system that was used to monitor induced pluripotent stem cell (iPSC) formation and subsequent differentiation. Our reporter cassette comprises less than 2.3 kb and remains functional during many cell passages after genomic integration. The fact that the human UTF1 genetic control elements work in a mouse background and the demonstrated functionality of the reporter in an epigenetic state further qualifies this system as a versatile new tool for iPSC research

Glycine decarboxylase activity drives non-small cell lung cancer tumor-initiating cells and tumorigenesis

10.1016/j.cell.2011.11.050Cell1481-2259-272CELL

Efficient endoderm induction from human pluripotent stem cells by logically directing signals controlling lineage bifurcations

10.1016/j.stem.2013.12.007Cell Stem Cell142237-25