Earthworm abundance and availability does not influence the reproductive decisions of black-tailed godwits in an agricultural grassland

Maintaining the biodiversity of agricultural ecosystems has become a global imperative. Across Europe, species that occupy agricultural grasslands, such as black-tailed godwits (Limosa limosa limosa), have undergone steep population declines. In this context, there is a significant need to both determine the root causes of these declines and identify actions that will promote biodiversity while supporting the livelihoods of farmers. Food availability, and specifically earthworm abundance (Lumbricidae), during the pre-breeding period has often been suggested as a potential driver of godwit population declines. Previous studies have recommended increasing the application of nitrogen to agricultural grasslands to enhance earthworm populations and aid agricultural production. Here we test whether food availability during the pre-breeding period affects when and where godwits breed. Using large-scale surveys of food availability, a long-term mark-recapture study, focal observations of foraging female godwits, and tracking devices that monitored godwit movements, we found little evidence of a relationship between earthworm abundance and the timing of godwit reproductive efforts or the density of breeding godwits. Furthermore, we found that the soils of intensively managed agricultural grasslands may frequently be too dry for godwits to forage for those earthworms that are present. The increased application of nitrogen to agricultural grasslands will therefore likely have no positive effect on godwit populations. Instead, management efforts should focus on increasing the botanical diversity of agricultural grasslands, facilitating conditions that prevent hardening soils, and reducing the populations of generalist predators. </ol

Quantitative multidimensional phenotypes improve genetic analysis of laterality traits

The UK Medical Research Council and Wellcome (Grant ref: 217065/Z/19/Z) and the University of Bristol provide core support for ALSPAC. This publication is the work of the authors and SP and JS will serve as guarantors for the analysis of the ALSPAC data presented in this paper. GWAS data were generated by Sample Logistics and Genotyping Facilities at Wellcome Sanger Institute and LabCorp (Laboratory Corporation of America) using support from 23andMe. Support to the genetic analysis was provided by the St Andrews Bioinformatics Unit funded by the Wellcome Trust [grant 105621/Z/14/Z]. The Hong Kong sample was funded through a Collaborative Research Fund from the Hong Kong Special Administrative Region Research Grants Council (CUHK8/CRF/13G, and C4054-17WF). JS is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, 418445085) and supported by the Wellcome Trust [Institutional Strategic Support fund, Grant number 204821/Z/16/Z]. SP is funded by the Royal Society (UF150663).Handedness is the most commonly investigated lateralised phenotype and is usually measured as a binary left/right category. Its links with psychiatric and neurodevelopmental disorders prompted studies aimed at understanding the underlying genetics, while other measures and side preferences have been less studied. We investigated the heritability of hand, as well as foot, and eye preference by assessing parental effects (n ≤ 5028 family trios) and SNP-based heritability (SNP-h2, n ≤ 5931 children) in the Avon Longitudinal Study of Parents and Children (ALSPAC). An independent twin cohort from Hong Kong (n = 358) was used to replicate results from structural equation modelling (SEM). Parental left-side preference increased the chance of an individual to be left-sided for the same trait, with stronger maternal than paternal effects for footedness. By regressing out the effects of sex, age, and ancestry, we transformed laterality categories into quantitative measures. The SNP-h2 for quantitative handedness and footedness was 0.21 and 0.23, respectively, which is higher than the SNP-h2 reported in larger genetic studies using binary handedness measures. The heritability of the quantitative measure of handedness increased (0.45) compared to a binary measure for writing hand (0.27) in the Hong Kong twins. Genomic and behavioural SEM identified a shared genetic factor contributing to handedness, footedness, and eyedness, but no independent effects on individual phenotypes. Our analysis demonstrates how quantitative multidimensional laterality phenotypes are better suited to capture the underlying genetics than binary traits.Publisher PDFPeer reviewe

Migration route, stopping sites, and non-breeding destinations of adult Black-tailed Godwits breeding in southwest Fryslân, The Netherlands

In this paper, we extend our understanding of the migration of Black-tailed Godwits (Limosa limosa limosa) by describing: (1) the orientation and geographic locations of individual migratory routes and (2) the spatial distribution of godwits across seasons and years. We accomplish this using satellite-tracking data from 36 adult godwits breeding in the 200-ha Haanmeer polder in The Netherlands, from 2015 to 2018. During both southward and northward migration, godwits used a narrow migratory corridor along which most individuals made stops within a network of sites, especially the Bay of Biscay, France and Doñana, Spain. Most sites were used consistently by the same individuals across years. However, sites in Morocco were used during northward migration by 75% of individuals, but not revisited by the same individual across years. After southward migration, a small proportion (15%) of godwits spent the entire non-breeding period north of the Sahara, but most (85%) crossed the Sahara and spent at least part of the non-breeding season among seven coastal sites in West Africa and one site in the Inner Niger Delta. Although site-use patterns varied among individuals, individuals showed high site fidelity and were consistent in the number of sites they used from year to year. The considerable differences in the spatial distribution of individuals that breed within a kilometre of one another raise questions about the causes and consequences of individual migratory differences. We discuss that full annual cycle tracking of juveniles from birth to adulthood is needed to understand the source of these individual differences. Our results on the spatial distribution of godwits throughout their annual cycle lay an important foundation of information that can be used to help conserve this declining species

Prevalence and heritability of handedness in a Hong Kong Chinese twin and singleton sample

Funding: Research Grants Council of the Hong Kong Special Administration Region (CUHK8/CRF/13G & C4054-17WF), by an internal grant entitled “Reading Development in Chinese and in English: Genetics and Neuroscience Correlates”(4930703) from The Chinese University of Hong Kong (CM is the PI on both grants), by a Hong Kong: Scotland Collaborative Research Partnership award from the Hong Kong Grants Council (CMis the PI for the Hong Kong side) and the Scottish Funding Council (SP is the PI for the Scotland side). It was additionally funded by an International Exchange Kan Tongo Po Visiting Fellowship to SP. SP is a Royal Society University Research Fellow.Background Left-handedness prevalence has been consistently reported at around 10% with heritability estimates at around 25%. Higher left-handedness prevalence has been reported in males and in twins. Lower prevalence has been reported in Asia, but it remains unclear whether this is due to biological or cultural factors. Most studies are based on samples with European ethnicities and using the preferred hand for writing as key assessment. Here, we investigated handedness in a sample of Chinese school children in Hong Kong, including 426 singletons and 205 pairs of twins, using both the Edinburgh Handedness Inventory and Pegboard Task. Results Based on a binary definition of writing hand, we found a higher prevalence of left-handedness (8%) than what was previously reported in Asian datasets. We found no evidence of increased left-handedness in twins, but our results were in line with previous findings showing that males have a higher tendency to be left-handed than females. Heritability was similar for both hand preference (21%) and laterality indexes (22%). However, these two handedness measures present only a moderate correlation (.42) and appear to be underpinned by different genetic factors. Conclusion In summary, we report new reference data for an ethnic group usually underrepresented in the literature. Our heritability analysis supports the idea that different measures will capture different components of handedness and, as a consequence, datasets assessed with heterogeneous criteria are not easily combined or compared.PreprintPublisher PDFPeer reviewe

Age-dependent timing and routes demonstrate developmental plasticity in a long-distance migratory bird

Longitudinal tracking studies have revealed consistent differences in the migration patterns of individuals from the same populations. The sources or processes causing this individual variation are largely unresolved. As a result, it is mostly unknown how much, how fast and when animals can adjust their migrations to changing environments. We studied the ontogeny of migration in a long-distance migratory shorebird, the black-tailed godwit Limosa limosa limosa, a species known to exhibit marked individuality in the migratory routines of adults. By observing how and when these individual differences arise, we aimed to elucidate whether individual differences in migratory behaviour are inherited or emerge as a result of developmental plasticity. We simultaneously tracked juvenile and adult godwits from the same breeding area on their south- and northward migrations. To determine how and when individual differences begin to arise, we related juvenile migration routes, timing and mortality rates to hatch date and hatch year. Then, we compared adult and juvenile migration patterns to identify potential age-dependent differences. In juveniles, the timing of their first southward departure was related to hatch date. However, their subsequent migration routes, orientation, destination, migratory duration and likelihood of mortality were unrelated to the year or timing of migration, or their sex. Juveniles left the Netherlands after all tracked adults. They then flew non-stop to West Africa more often and incurred higher mortality rates than adults. Some juveniles also took routes and visited stopover sites far outside the well-documented adult migratory corridor. Such juveniles, however, were not more likely to die. We found that juveniles exhibited different migratory patterns than adults, but no evidence that these behaviours are under natural selection. We thus eliminate the possibility that the individual differences observed among adult godwits are present at hatch or during their first migration. This adds to the mounting evidence that animals possess the developmental plasticity to change their migration later in life in response to environmental conditions as those conditions are experienced

Dyslexia-related loci are significantly associated with language and literacy in Chinese–English bilingual Hong Kong Chinese twins

This study was partially funded by the Research Grants Council of the Hong Kong Special Administration Region (C4054-17WF) and the Theme-based Research Scheme from the Hong Kong Special Administrative Region Research Grants Council (T44-410/21-N).A recent genome-wide association study on dyslexia in 51,800 affected European adults and 1,087,070 controls detected 42 genome-wide significant single nucleotide variants (SNPs). The association between rs2624839 in SEMA3F and reading fluency was replicated in a Chinese cohort. This study explores the genetic overlap between Chinese and English word reading, vocabulary knowledge and spelling, and aims at replicating the association in a unique cohort of bilingual (Chinese–English) Hong Kong Chinese twins. Our result showed an almost complete genetic overlap in vocabulary knowledge (r2 = 0.995), and some genetic overlaps in word reading and spelling (r2 = 0.846, 0.687) across the languages. To investigate the region near rs2624839, we tested proxy SNPs (rs1005678, rs12632110 and rs12494414) at the population level (n = 305–308) and the within-twin level (n = 342–344 [171–172 twin pairs]). All the three SNPs showed significant associations with quantitative Chinese and English vocabulary knowledge (p PostprintPeer reviewe

A redshift distortion free correlation function at third order in the nonlinear regime

The zeroth-order component of the cosine expansion of the projected three-point correlation function is proposed for clustering analysis of cosmic large scale structure. These functions are third order statistics but can be measured similarly to the projected two-point correlations. Numerical experiments with N-body simulations indicate that the advocated statistics are redshift distortion free within 10% in the non-linear regime on scales ~0.2-10Mpc/h. Halo model prediction of the zeroth-order component of the projected three-point correlation function agrees with simulations within ~10%. This lays the ground work for using these functions to perform joint analyses with the projected two-point correlation functions, exploring galaxy clustering properties in the framework of the halo model and relevant extensions.Comment: 10 pages, 6 figs; MNRAS accepte