13 research outputs found
Identificação laboratorial de micobactérias em amostras respiratórias de pacientes HIV-positivos com suspeita de tuberculose
Phlebotomine fauna, natural infection rate and feeding habits of Lutzomyia cruzi in Jaciara, state of Mato Grosso, Brazil
Essential oils from Betula lenta, Cinnamomum cassia, Citrus aurantium var. Amara and Acorus calamus as biopesticides against cowpea weevil
Prevalence of and factors related to tuberculosis in seropositive human immunodeficiency virus patients at a reference center for treatment of human immunodeficiency virus in the southern region of the state of Rio Grande do Sul, Brazil
Objective: In view of the relevance of co-infection with tuberculosis and human immunodeficiency virus, this study was
designed to determine tuberculosis prevalence and identify factors related to tuberculosis in patients residing in a region in which both infections are highly prevalent. Methods: All patients treated during 1999 at the HIV/AIDS Clinic of the Universidade Federal do Rio Grande (Rio Grande Federal University) University Hospital were evaluated retrospectively, from the time of human immunodeficiency virus diagnosis, in terms of the incidence of tuberculosis and its relationship to sociodemographic, behavioral and immunological factors. Results: The sample included 204 patients, and tuberculosis prevalence was found to be 27%. The multivariate analysis showed a significant correlation between the development of tuberculosis and being of African descent (odds ratio: 4.76; 95% confidence interval: 1.93-11.72) and an inverse correlation between the development of tuberculosis and the TCD4+ lymphocyte count at the time of human immunodeficiency virus diagnosis (odds ratio: 0.995; 95% confidence interval: 0.993-0.997). When analyzed separately,other variables were found to be potential risk factors: being of the male gender (odds ratio: 2.49; 95% confidence interval: 1.15-5.39); and using illicit drugs (odds ratio: 2.1; 95% confidence interval: 1.02-4.31). Conclusion: The factors responsible for the development of tuberculosis among patients who are human immunodeficiency virus seropositive include immunological, socioeconomic and demographic factors. The high rate of tuberculosis prevalence among the seropositive patients underscores the urgent need to implement strategies that combine rapid identification and prompt treatment of individuals with active or latent infection, as well as of those with whom they have been in contact
Effect of low-dose gaseous ozone on pathogenic bacteria
<p>Abstract</p> <p>Background</p> <p>Treatment of chronically infected wounds is a challenge, and bacterial environmental contamination is a growing issue in infection control. Ozone may have a role in these situations. The objective of this study was to determine whether a low dose of gaseous ozone/oxygen mixture eliminates pathogenic bacteria cultivated in Petri dishes.</p> <p>Methods</p> <p>A pilot study with 6 bacterial strains was made using different concentrations of ozone in an ozone-oxygen mixture to determine a minimally effective dose that completely eliminated bacterial growth. The small and apparently bactericidal gaseous dose of 20 μg/mL ozone/oxygen (1:99) mixture, applied for 5min under atmospheric pressure was selected. In the 2<sup>nd</sup> phase, eight bacterial strains with well characterized resistance patterns were evaluated <it>in vitro</it> using agar-blood in adapted Petri dishes (10<sup>5</sup> bacteria/dish). The cultures were divided into 3 groups: 1- ozone-oxygen gaseous mixture containing 20 μg of O<sub>3</sub>/mL for 5 min; 2- 100% oxygen for 5 min; 3- baseline: no gas was used.</p> <p>Results</p> <p>The selected ozone dose was applied to the following eight strains: <it>Escherichia coli</it>, oxacillin-resistant <it>Staphylococcus aureus</it>, oxacillin-susceptible <it>Staphylococcus aureus</it>, vancomycin-resistant <it>Enterococcus faecalis</it>, extended-spectrum beta-lactamase-producing <it>Klebsiella pneumoniae</it>, carbapenem-resistant <it>Acinetobacter baumannii</it>, <it>Acinetobacter baumannii</it> susceptible only to carbapenems, and <it>Pseudomonas aeruginosa</it> susceptible to imipenem and meropenem. All isolates were completely inhibited by the ozone-oxygen mixture while growth occurred in the other 2 groups.</p> <p>Conclusion</p> <p>A single topical application by nebulization of a low ozone dose completely inhibited the growth of all potentially pathogenic bacterial strains with known resistance to antimicrobial agents.</p