Nutritional evaluation of fermented palm kernel cake using red tilapia

The use of palm kernel cake (PKC) and other plant residues in fish feeding especially under extensive aquaculture have been in practice for a long time. On the other hand, the use of microbial-based feedstuff is increasing. In this study, the performance of red tilapia raised on Trichoderma longibrachiatum fermented PKC (TL-PKC) was evaluated. Seven isonitrogenous and isocaloric diets were formulated. Reference diet, D1 had no PKC, while the other diets, D2 to D5 contained 10, 20, 30and 40% fermented PKC (TL-PKC), respectively. All diets contained 1% chromic oxide as an inert marker. Red tilapias of average weight of 2.5 g were fed on these six diets for a period of 8 weeks. The fish were fed at 4% of their body weight, twice a day. There was no difference in mortality rate of fish on the various diets used. However, weight gain decreased with an increase of TL-PKC in diets. There were significant (P £ 0.05) differences in the apparent digestibility coefficient (ADC) of protein and drymatter between the reference diet and diet containing TL-PKC. The ADC of both protein and dry matter generally decrease when the percentage TL-PKC was increased in the test diets. There was also no significant difference in carcass protein content among fish on the various diets. However, there was a significant (P £ 0.05) increase in the levels of phosphorus, calcium and copper in the carcass of fish raised on TL-PKC, but the level of lipids was significantly reduced. Decreasing dry matter and protein digestibility with corresponding weight reduction may have resulted from increased crude fibre content of diets with TL-PKC

A quick DNA extraction protocol: Without liquid nitrogen in ambient temperature

Marker assisted selection is an effective technique for quality traits selection in breeding program which are impossible by visual observation. Marker assisted selection in early generation requires rapid DNA extraction protocol for large number of samples in a low cost approach. A rapid and inexpensive DNA extraction protocol has been described for different tissues of color rice and other plant species which contain pigment and polyphenolic compound. This method has been modified from well known cetyltrimethylammonium bromide (CTAB) method where CTAB is used for DNA extraction. This protocol is simple and fast compared to other methods and no liquid nitrogen is required. Only inexpensive chemicals and ordinary laboratory equipments are enough for DNA extraction. The quantity of total genomic DNA from different tissues was almost similar which was extracted from 10 mg samples. The extracted DNA is stable and applicable to marker assisted selection, DNA fingerprinting, quantitative traits loci analysis, screening of transformants and enzymatic digestion.Key words: Different plant tissues, inexpensive, rice flour, rapid DNA extraction

Growth, yield and fiber morphology of kenaf (Hibiscus cannabinus L.) grown on sandy bris soil as influenced by different levels of carbon

The effects of carbon levels on plant growth, yield and fiber morphological properties are not available for kenaf that is considered as a potential source of low cost natural fiber and feedstock for energy production as well. A pot-culture experiment was conducted in shade house to determine the effects of carbon levels on plant growth, yield and fiber morphology of different kenaf varieties. The plants of five kenaf varieties were grown in pots containing sandy beach ridges interspersed with swales (BRIS) soil. Organic carbons at the levels of 0, 10, 20, 30 and 40 t ha-1 were applied to pots using organic fertilizer. At harvest, stem diameter, plant height, leaf number, leaf area, plant components biomass, bast and core fiber yield and fiber dimensional properties were determined. Maximum growth, dry matter and fiber yield, and morphological characters were achieved at the carbon levels 20 t ha-1 but the values of these parameters decreased with additional increase in carbon levels. Among the varieties, HC2 had the highest plant height, leaf biomass, total dry matter and fiber yield. The longest bast fiber was observed in variety HC2. The variety G4 showed the widest fiber and higher lumen width among others. The bast fibers of all the kenaf verities were longer than core fiber. The core fiber was wider and higher in lumen width than the bast fiber. The above results keep a significant role and would be useful to select better varieties of kenaf for the purpose of making quality paper and paper products and to grow kenaf on BRIS soil with adequate yield and fibre quality using better management of organic carbon.Key words: Kenaf varieties, carbon levels, growth, yield, fiber morphology, beach ridges interspersed with swales (BRIS) soil

Effects of carbon levels on shoot growth and root characteristics of different kenaf (Hibiscus cannabinus L.) varieties grown on sandy bris soil

The effects of carbon levels on shoot growth and root characteristics of five kenaf varieties were assessed in a shade house experiment. The kenaf plants were grown in pots containing sandy beach ridges interspersed with swales (BRIS) soil. Organic carbons at levels of 0, 10, 20 and 30 t ha-1 were applied to pots using organic fertilizer. The plants at carbon levels 20 t ha-1 had the highest plant height, leaf area, shoot and root dry matter, total root length, root surface area, total root volume, and number of root tips. Increasing carbon levels had negatively affected shoot growth and root morphology of all varieties under study. There was an obvious difference in response to carbon levels among the five varieties with HC2 showing the highest performance in terms of shoot growth and root characteristics. These results provide significant insights into limitations and opportunities for growing of kenaf in BRIS soil with better organic carbon management.Key words: Kenaf varieties, carbon levels, root traits, root and shoot growth, sandy BRIS soil

The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice

Mature seeds of four upland rice cultivars namely Kusan, Lamsan, Selasi and Siam were assessed for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators, incorporated into MS (Murashige and Skoog) basal medium. Callus induction frequency was significantly different among the cultivars, as well as among the 2,4-Dichlorophenoxyacetic acid (2,4-D) levels tested. All tested cultivars exhibited highest callus frequency at 2 mgl-1 2,4-D. The incorporation of -naphthaleneacetic acid (NAA) and kinetin (Kin) in the callus induction medium supplemented with 2 mgl-1 2,4-D did not significantly improve the callus induction frequency. After two subcultures, at 24 days interval, the best response to callus induction was from cultivar Selasi, while callus browning became prominent in cultivars Kusan and Siam. Embryogenic callus placed on different regeneration media exhibited the highest regeneration frequency on medium containing 0.5 mgl-1 NAA + 2.0 mgl-1 Kin + 2.0 mgl-1 6-benzylaminopurine (BAP). The maximum regeneration frequency was achieved in cultivar Selasi followed by Lamsan while Siam and Kusan exhibited poor regeneration response. Among the four upland rice cultivars evaluated, Selasi and Lamsan are two promising cultivars in terms of callus induction frequency and morphology, and regeneration ability of the embryogenic callus

Genetic diversity in grain quality and nutrition of aromatic rices

The study was conducted at Bangladesh Rice Research Institute (BRRI), Gazipur in 2005 to assess the genetic divergence of aromatic rices for grain quality and nutrition aspects. Forty genotypes composedof 32 local aromatic, five exotic aromatic and three non-aromatic rice varieties were used. Univariate and multivariate analyses were done. Enormous variations were observed in majority of characters viz.grain length, breadth, kernel weight, milling yield, kernel length, L/B ratio of kernel, volume expansion ratio (VER), protein content, amylose content, elongation ratio (ER) and cooking time. In multivariateanalysis, genotypes were grouped into six clusters. In the discriminant function analysis (DFA), function 1 alone absorbed 61.7% of the total variance. The most contributing variables were kernel weight, kernel length and L/B ratio in function 1. The inter-cluster D2 value was maximum (26.53) between I and VI followed by 21.28 (between I and V). Minimum D2 value was found (5.90) between II and III. Majority of the local aromatic rice varieties with smaller kernels were included in the cluster I. The cluster III contains Elai, sarwati and sugandha-1 with long-slender kernel and ‘very good’ appearance. Thus, these varieties can be used in breeding programme for improvement of germplasms in cluster-I

Effect of salt stress on germination and early seedling growth of rice (Oryza sativa L.)

The response of twelve rice varieties against six salinity levels (0, 4, 8, 12, 16 and 20 dS m-1) were studied at germination and early seedling stages. Data were analyzed using SAS and means were separated by LSD for final germination percentage (FGP), speed of germination (SG), germinationenergy percentage (GE%), plumule and radical length and plumule and radical dry weight. Based on dry matter yield reduction, rice varieties were classified as tolerant (T), moderately tolerant (MT), moderately susceptible (MS) or susceptible (S). Germination was completely arrested at 20 dS m-1 salt concentration. Salinity decreased FGP, SG, GE % and led to reduction in shoot and root length and dry weight in all varieties and the magnitude of reduction increased with increasing salinity stress. Ricevarieties MR211, IR20, BR40 and MR232 showed greater salt tolerance during germination (germinated at 12 dS m-1 salinity). However, MR211, MR232 and IR20 performed better based on dry matter yield reduction. The result suggested that MR211, MR232 and IR20 might be used for further study of salinity effect on growth processes and physiological consequences at advanced stage of growth, since salt tolerance of a crop at germination and early seedling stage may not correspond to that at advanced stage

Virus free seed potato production through sprout cutting technique under net-house

In order to evaluate the performance of sprout cutting for seed potato production against virus infection, a study was conducted at Bangladesh Agricultural Development Corporation (BADC) Foundation Seed Potato Production Farm, Domar, Nilphamary, Bangladesh in 2005 - 2006. Sprout cut seedlings were grown under three production practices viz. Net-house practice, BADC practice and farmers’ practice. Sprout cutting technique was proved to be an effective method of seed potato production against potato virus Y (PVY) and potato leaf roll virus (PLRV). The incidence of PVY and PLRV was detected as nil in net-house practice. However, PVY and PLRV were prevalent in BADC practice and farmers’ practice although, BADC practice performed better over farmers’ practice. Yield of seed potato and non-seed tubers along with yield attributes were found to be higher in all the five varieties (Diamant, Baraka, Asterix, Raja and Provento) in net-house practice followed by BADC practice and farmers’ practice. Among the varieties Diamant, Asterix and Raja seemed to be better in performance as compared to the others. In most of the cases net-house practice differed significantly (p = 0.05) with the others.Keywords: Potato virus Y, potato leaf roll virus, seed potato, sprout cuttingAfrican Journal of Biotechnology Vol. 9(36), pp. 5852-5858, 6 September, 201

Phytochemical fingerprint and biological activities of three Malaysian Ficus Deltoidea Cultivars

Background: Ficus deltoidea(Moraceae), is a Malay Traditional Medicine locally known as Mas Cotek. Three varieties (angustifolia, deltoidea and kunslerii) has been indictincty used. Aims We here aim to better understand their chemistry and bioactivities to inform future scientific and agronomic research. Methods: We extracted and analyzed (HPTLC and HPLC-UV) samples from these varieties. The in vitro screening included the scavenging of DPPH and NO radicals, activity upon tyrosinase and cytotoxicity against three human prostate cancer cells (PC3, DU145 and LNCaP)using the sulforhodamine B proliferation assay and the MTT mitochondrial viability assay. Results : We show that vitexin, orientin and isoorientin may act as intraspecific and interorgan phytomarkers The biological activities of the extracts point out to the antioxidant value of extracts from the deltoidea and kunslerii varieties whilst the inhibition of tyrosinase is only present in the root extract of the var. deltoidea which is also endowed with cytotoxic activity against prostate cancer cells. Conclusion: We suggest that the three Malaysian Ficus deltoideabotanical varieties (angustifolia, deltoideaand kunslerii) can be considered chemovars. The most active extract was from the roots of var. deltoids that show antioxidant, antimelanogenic and cytotoxic potential

Evaluation of growth and yield potentialities of local boro rice varieties in south-west region of Bangladesh

A field experiment was conducted to evaluate the growth and yield performance of local boro rice varieties. Twelve local boro rice varieties were included in this study namely Nayon moni, Tere bale, Bere ratna, Ashan boro, Kajol lata, Koijore, Kali boro, Bapoy, Latai balam, Choite boro, GS one and Sylhety boro. Growth parameters viz. plant height and number of tillers hill-1(at different days after transplanting); yield contributing characters such as effective tillers hill-1, panicle length, number of grains panicle-1, filled grains panicle-1, thousand grain weight, grain yield, straw yield, biological yield and harvest index were recorded. The result revealed that the plant height and number of tillers hill-1 at different days after transplanting varied significantly among the varieties. The plant height for all the varieties and number of tillers hill-1 for most of the varieties increased up to harvest. At harvest, the tallest plant (123.80 cm) was recorded in Bapoy and the shortest (81.13 cm) was found in GS one. The maximum number of tillers hill-1 (46.00) was observed in Sylhety boro and the minimum (19.80) in Bere ratna. All of the parameters of yield and yield contributing characters differed significantly at 1% level except grain yield, biological yield and harvest index. The maximum number of effective tillers hill-1 (43.87) was recorded in the variety Sylhety boro and the minimum (17.73) was found in Bere ratna. The highest (110.57) and the lowest (42.13) number of filled grains panicle-1 was observed in the variety Koijore and Sylhety boro, respectively. Thousand grain weight was the highest (26.35g) in Kali boro and the lowest (17.83g) in GS one. Grain yield was not differed significantly among the varieties but numerically the highest grain yield (5.01 t ha-1) was found in the variety Koijore and the lowest in GS one(3.17 t ha-1). Considering all parameters the varieties Koijore (5.01tha-1), Choite boro, Sylhety boro, Ashan boro, Bere ratna performed better for the southwest region of Bangladesh