36 research outputs found

    Activity-Induced Remodeling of Olfactory Bulb Microcircuits Revealed by Monosynaptic Tracing

    Get PDF
    The continued addition of new neurons to mature olfactory circuits represents a remarkable mode of cellular and structural brain plasticity. However, the anatomical configuration of newly established circuits, the types and numbers of neurons that form new synaptic connections, and the effect of sensory experience on synaptic connectivity in the olfactory bulb remain poorly understood. Using in vivo electroporation and monosynaptic tracing, we show that postnatal-born granule cells form synaptic connections with centrifugal inputs and mitral/tufted cells in the mouse olfactory bulb. In addition, newly born granule cells receive extensive input from local inhibitory short axon cells, a poorly understood cell population. The connectivity of short axon cells shows clustered organization, and their synaptic input onto newborn granule cells dramatically and selectively expands with odor stimulation. Our findings suggest that sensory experience promotes the synaptic integration of new neurons into cell type-specific olfactory circuits

    Impact of Treadmill Running and Sex on Hippocampal Neurogenesis in the Mouse Model of Amyotrophic Lateral Sclerosis

    Get PDF
    Hippocampal neurogenesis in the subgranular zone (SGZ) of dentate gyrus (DG) occurs throughout life and is regulated by pathological and physiological processes. The role of oxidative stress in hippocampal neurogenesis and its response to exercise or neurodegenerative diseases remains controversial. The present study was designed to investigate the impact of oxidative stress, treadmill exercise and sex on hippocampal neurogenesis in a murine model of heightened oxidative stress (G93A mice). G93A and wild type (WT) mice were randomized to a treadmill running (EX) or a sedentary (SED) group for 1 or 4 wk. Immunohistochemistry was used to detect bromodeoxyuridine (BrdU) labeled proliferating cells, surviving cells, and their phenotype, as well as for determination of oxidative stress (3-NT; 8-OHdG). BDNF and IGF1 mRNA expression was assessed by in situ hybridization. Results showed that: (1) G93A-SED mice had greater hippocampal neurogenesis, BDNF mRNA, and 3-NT, as compared to WT-SED mice. (2) Treadmill running promoted hippocampal neurogenesis and BDNF mRNA content and lowered DNA oxidative damage (8-OHdG) in WT mice. (3) Male G93A mice showed significantly higher cell proliferation but a lower level of survival vs. female G93A mice. We conclude that G93A mice show higher hippocampal neurogenesis, in association with higher BDNF expression, yet running did not further enhance these phenomena in G93A mice, probably due to a ‘ceiling effect’ of an already heightened basal levels of hippocampal neurogenesis and BDNF expression

    Key enzymes catalyzing glycerol to 1,3-propanediol

    Full text link

    Effects of salinity and possible interactions with temperature and pH on growth and photosynthesis of Halophila johnsonii Eiseman

    Get PDF
    The effects of salinity, temperature, and pH variations on growth, survival, and photosynthetic rates of the seagrass Halophila johnsonii Eiseman were examined. Growth and survival responses to salinity were characterized by aquarium experiments in which plants were exposed to seven different salinity treatments (0, 10, 20, 30, 40, 50, and 60 psu) during 15 days. Photosynthetic behavior was assessed for short-term salinity exposures (1 or 20 h) by incubation experiments in biological oxygen demand (BOD) bottles and by measuring photosynthesis versus irradiance (PI) responses in an oxygen electrode chamber. In the bottle experiments the possible effects of interactions between salinity and temperature (15, 25, and 35°C) or pH (5, 6, 7, and 8.2) were also examined. Growth and survival of H. johnsonii were significantly affected by salinity, with maximum rates obtained at 30 psu. Salinity also altered the parameters of the PI curves. Light saturated photosynthesis (Pmax) and the photosynthetic efficiency at subsaturating light (α) increased significantly up to an optimum of 40 psu, decreasing again at the highest salinities. Dark respiration rates and compensating irradiance (Ic) showed minimum values at 40 and 50 psu, while light-saturation point (Ik) was maximum at 30–50 psu. An interaction between salinity and temperature was not found although an increase of temperature alone produced an increase in α, Pmax, respiration rates, and Ik. An interaction between salinity and pH was only found in the Pmax response: Pmax increased with pH=5 at 30 psu. In addition, reducing the pH increased α significantly. In the BOD bottles experiment a significant reduction in the dark respiration with decreasing pH was observed, but the opposite trend was observed in the photosynthetic rate. These results suggest that the endemic seagrass H. johnsonii could be negatively affected by hypo- or hypersalinity conditions, although salinity changes did not seem to alter the tolerance of this species to other environmental factors, such as temperature or pH.This research was supported by a grant of the Generalitat Valenciana (CTESPR/2002/59)

    Responses of two Mediterranean seagrasses to experimental changes in salinity

    Get PDF
    The aim of this study is to examine the effects of variations in salinity levels on growth and survival of two fast-growing Mediterranean seagrasses, Cymodocea nodosa and Zostera noltii. We also tested the capacity of C. nodosa to acclimate to a gradual increase in salinity and to discover how it responds to a sharp rise in salinity in combination with other factors, such as increases in temperature, seasonality and different plant-population origins. Several short-term (10 days) experiments were conducted under controlled conditions. For each experiment, ten marked shoots were placed in 5-l aquaria, where they were exposed to different salinity treatments (ranging from 2 to 72 psu). Growth and survival of both species were significantly affected by salinity. A significant effect between salinity and temperature on the shoot growth rate of C. nodosa was also detected, but not on shoot mortality. When C. nodosa plants were acclimated by gradually increasing the salinity level, it was observed that acclimatisation improved tolerance to salinity changes. A different response to salinity variations, depending on the origin of the plants or the season of the year, was also detected. These results indicated that Z. noltii plants tolerate conditions of hyposalinity better than C. nodosa, and that the tolerance range of C. nodosa may change depending on the temperature, the season or the population.This research was financed by an ACUAMED contract and by an FPI grant (FPI 01 A 002) from the Generalitat Valenciana
    corecore