The non-coding transcriptome as a dynamic regulator of cancer metastasis.

Since the discovery of microRNAs, non-coding RNAs (NC-RNAs) have increasingly attracted the attention of cancer investigators. Two classes of NC-RNAs are emerging as putative metastasis-related genes: long non-coding RNAs (lncRNAs) and small nucleolar RNAs (snoRNAs). LncRNAs orchestrate metastatic progression through several mechanisms, including the interaction with epigenetic effectors, splicing control and generation of microRNA-like molecules. In contrast, snoRNAs have been long considered "housekeeping" genes with no relevant function in cancer. However, recent evidence challenges this assumption, indicating that some snoRNAs are deregulated in cancer cells and may play a specific role in metastasis. Interestingly, snoRNAs and lncRNAs share several mechanisms of action, and might synergize with protein-coding genes to generate a specific cellular phenotype. This evidence suggests that the current paradigm of metastatic progression is incomplete. We propose that NC-RNAs are organized in complex interactive networks which orchestrate cellular phenotypic plasticity. Since plasticity is critical for cancer cell metastasis, we suggest that a molecular interactome composed by both NC-RNAs and proteins orchestrates cancer metastasis. Interestingly, expression of lncRNAs and snoRNAs can be detected in biological fluids, making them potentially useful biomarkers. NC-RNA expression profiles in human neoplasms have been associated with patients' prognosis. SnoRNA and lncRNA silencing in pre-clinical models leads to cancer cell death and/or metastasis prevention, suggesting they can be investigated as novel therapeutic targets. Based on the literature to date, we critically discuss how the NC-RNA interactome can be explored and manipulated to generate more effective diagnostic, prognostic, and therapeutic strategies for metastatic neoplasms

The value of genetic resources in agriculture: a meta-analysis assessing existing knowledge and future research needs

The protection of genetic resources in agriculture is an important aspect of biodiversity conservation. Knowledge of the value of genetic resources can contribute to determining the appropriate focus and extent of conservation. This study reviewed and summarised literature on the economic value of genetic resources using meta-analysis. Altogether, 22 studies were used to describe current knowledge on the value of genetic resources. Furthermore, 14 studies with 93 value observations were examined with a meta-regression model to identify variables that explain the willingness-to-pay (WTP) for or willingness-to-accept (WTA) loss of genetic resources. Grain genetic resources were ascribed lower value compared to animal genetic resources (AnGR) and agrobiodiversity, and the values of breeds or varieties and conservation programmes were higher than the value of individual attributes. Future research should address the gaps in knowledge that are relevant for policy-making. This particularly includes improving knowledge on the value of plant genetic resources (PGR), obtaining value estimates for maintaining genetic diversity in Europe and the United States, estimating the relative magnitude of use and non-use values and determining the value consumers place on genetic resources and diversity in agriculture. An extensive database with valuation literature on genetic resources that fulfils the requirements for benefit transfer is essential to utilise value information more efficiently in decision-making situations.v2011o

Viral and Bacterial Interactions in the Upper Respiratory Tract

<p>Respiratory infectious diseases are mainly caused by viruses or bacteria that often interact with one another. Although their presence is a prerequisite for subsequent infections, viruses and bacteria may be present in the nasopharynx without causing any respiratory symptoms. The upper respiratory tract hosts a vast range of commensals and potential pathogenic bacteria, which form a complex microbial community. This community is assumed to be constantly subject to synergistic and competitive interspecies interactions. Disturbances in the equilibrium, for instance due to the acquisition of new bacteria or viruses, may lead to overgrowth and invasion. A better understanding of the dynamics between commensals and pathogens in the upper respiratory tract may provide better insight into the pathogenesis of respiratory diseases. Here we review the current knowledge regarding specific bacterial-bacterial and viral-bacterial interactions that occur in the upper respiratory niche, and discuss mechanisms by which these interactions might be mediated. Finally, we propose a theoretical model to summarize and illustrate these mechanisms.</p>