Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth

Male moths use species-specific sex pheromones to identify and orientate toward conspecific females. Odorant receptors (ORs) for sex pheromone substances have been identified as sex pheromone receptors in various moth species. However, direct in vivo evidence linking the functional role of these ORs with behavioural responses is lacking. In the silkmoth, Bombyx mori, female moths emit two sex pheromone components, bombykol and bombykal, but only bombykol elicits sexual behaviour in male moths. A sex pheromone receptor BmOR1 is specifically tuned to bombykol and is expressed in specialized olfactory receptor neurons (ORNs) in the pheromone sensitive long sensilla trichodea of male silkmoth antennae. Here, we show that disruption of the BmOR1 gene, mediated by transcription activator-like effector nucleases (TALENs), completely removes ORN sensitivity to bombykol and corresponding pheromone-source searching behaviour in male moths. Furthermore, transgenic rescue of BmOR1 restored normal behavioural responses to bombykol. Our results demonstrate that BmOR1 is required for the physiological and behavioural response to bombykol, demonstrating that it is the receptor that mediates sex pheromone responses in male silkmoths. This study provides the first direct evidence that a member of the sex pheromone receptor family in moth species mediates conspecific sex pheromone information for sexual behaviour

Fundamental Consideration on the Guide Systems to Parking-lots in the central district of Fukui

In this study， the main aim is to make informations for the Guide Systems to Parking-lots， which guides car drivers to usable parking-lots smoothly in the central district of Fukui. At first， the parking demand in the central district of Fukui is estimated. Secondly， "Questionaire Survey on the use of parking-lots" for car drivers in the central district of Fukui is carried out. Then， several problems in this district are discussed. Finally， Computer Aided Calculation， introducing the simplified guide systems to parking-lots， is carried out， and the effects of introduction are investigated

Slc3a2 Mediates Branched-Chain Amino-Acid-Dependent Maintenance of Regulatory T Cells

Foxp3+ regulatory T (Treg) cells, which suppress immune responses, are highly proliferative in vivo. However, it remains unclear how the active replication of Treg cells is maintained in vivo. Here, we show that branched-chain amino acids (BCAAs), including isoleucine, are required for maintenance of the proliferative state of Treg cells via the amino acid transporter Slc3a2-dependent metabolic reprogramming. Mice fed BCAA-reduced diets showed decreased numbers of Foxp3+ Treg cells with defective in vivo proliferative capacity. Mice lacking Slc3a2 specifically in Foxp3+ Treg cells showed impaired in vivo replication and decreased numbers of Treg cells. Slc3a2-deficient Treg cells showed impaired isoleucine-induced activation of the mTORC1 pathway and an altered metabolic state. Slc3a2 mutant mice did not show an isoleucine-induced increase of Treg cells in vivo and exhibited multi-organ inflammation. Taken together, these findings demonstrate that BCAA controls Treg cell maintenance via Slc3a2-dependent metabolic regulation

2004ネン ナンキョク ニオケル イリョウ イガク ケンキュウ ニカンスル ケンキュウ シュウカイ ノ ホウコク

004年の「南極における医療・医学研究集会」が8月28日,国立極地研究所で開催された.主な内容は「南極の高所がヒトに与える影響」,「南極でのストレスがアミノ酸脂肪酸代謝に与える影響」,「越冬基地におけるレジオネラ菌調査」,「南極における紫外線障害とその対策」,「46次隊の医学研究計画」であった.16施設から合計22名が参加し,口頭発表10演題,ポスター発表1演題,文書発表1演題について,活発な討論が行われた.A meeting on Antarctic Medical Research was held at NIPR on August 28, 2004. Twenty two members participated in the meeting with ten oral presentations, one poster presentation and one report paper. The main subjects were The human responses in the Antarctic highland", The influence of the cold stress on the amino acids and lipid metabolism", Legionella contamination at the Antarctic wintering stations". The JARE-46 medical research program" was also reported and discussed

Current Performance and On-Going Improvements of the 8.2 m Subaru Telescope

An overview of the current status of the 8.2 m Subaru Telescope constructed and operated at Mauna Kea, Hawaii, by the National Astronomical Observatory of Japan is presented. The basic design concept and the verified performance of the telescope system are described. Also given are the status of the instrument package offered to the astronomical community, the status of operation, and some of the future plans. The status of the telescope reported in a number of SPIE papers as of the summer of 2002 are incorporated with some updates included as of 2004 February. However, readers are encouraged to check the most updated status of the telescope through the home page, http://subarutelescope.org/index.html, and/or the direct contact with the observatory staff.Comment: 18 pages (17 pages in published version), 29 figures (GIF format), This is the version before the galley proo

2006ネン ナンキョク イガク イリョウ ケンキュウ シュウカイ ホウコク

南極医学医療研究集会は,わが国の南極医学研究と医療問題についての研究成果を報告・討論し,次期の観測隊における医学研究に寄与することを目的として毎年行われている.2006年の本研究集会は8月26日,国立極地研究所講堂で行われた.27施設から42名が参加し18の演題報告がなされ,近年では最大規模の研究集会となった. 参加者は越冬経験医師をはじめ,共同研究を行っている大学や研究機関の研究者,関連領域の研究を行っている宇宙開発機構やスポーツ科学研究所などの研究者,南極に興味のある一般病院の臨床医など多彩であった. 2004年より昭和基地に導入されたテレビ会議システムを活用して,昭和基地の医師もリアルタイム映像で討論に参加した.また韓国,中国の越冬医師が初めて参加した.これは3カ国の極地研究所による事前の準備と連携により実現した.集会では各国の南極基地の医療状況や医学研究活動が報告され,活発な意見交換がなされた.南極医学医療研究分野におけるアジア連携の端緒となることが期待される.A workshop on Antarctic Medical Research and Medicine 2006 was held at the National Institute of Polar Research (NIPR) on 26 August, 2006. Forty two participants from 27 institutes attended. The members consist of medical doctors with Antarctic experience, human biologists, research scientists in other fields, logistic staff members of the expedition and also medical doctors interested in Antarctica. The current resident doctor at Syowa Station joined the discussion through a telecommunication system. Doctors with Antarctic experience from China and Korea also participated in the workshop. They gave presentations on their Antarctic activities, followed by an active discussion session. Eighteen presentations were given on various topics, including the International Polar Year (IPY) 2007-2008 in medical research, space medicine, telemedicine, an international comparative study of medical operations, psychological surveys, Antarctic high-altitude medicine, Legionella surveillance and nutritional studies

Refinement of ectopic protein expression through the GAL4/UAS system in Bombyx mori: Application to behavioral and developmental studies

金沢大学理工研究域自然システム学系Silkmoth, Bombyx mori, is one of the important model insects in which transgenic techniques and the GAL4/UAS system are applicable. However, due to cytotoxicity and low transactivation activity of GAL4, effectiveness of the GAL4/UAS system and its application in B. mori are still limited. In the present study, we refined the previously reported UAS vector by exploiting transcriptional and translational enhancers, and achieved 200-fold enhancement of reporter GFP fluorescence in the GAL4/UAS system. Enhanced protein expression of membrane-targeted GFP and calcium indicator protein (GCaMP5G) drastically improved visualization of fine neurite structures and neural activity, respectively. Also, with the refined system, we generated a transgenic strain that expresses tetanus toxin light chain (TeTxLC), which blocks synaptic transmission, under the control of GAL4. Ectopic TeTxLC expression in the sex pheromone receptor neurons inhibited male courtship behavior, proving effectiveness of TeTxLC on loss-of-function analyses of neural circuits. In addition, suppression of prothoracicotropic hormone (PTTH) or insulin-like peptide (bombyxin) secretion impaired developmental timing and growth rate, respectively. Furthermore, we revealed that larval growth is sex-differentially regulated by these peptide hormones. The present study provides important technical underpinnings of transgenic approaches in silkmoths and insights into mechanisms of postembryonic development in insects. © 2017 The Author(s)

“Judo Health Exercise” development and planning to spread throughout Japan

J-GLOBAL ID : 200901043284363204J-GLOBAL ID : 200901004099122068J-GLOBAL ID : 202201010240591568J-GLOBAL ID : 201001039879999076J-GLOBAL ID : 200901050705583957We have developed a physical exercise named “Judo Health Exercise” base on “Judo” throwing techniques such as “Seoi-nage”, “O-goshi”, “O-soto-gari”, “Uchimata”, and “Ashi-harai”. This exercise aims at prevention of dementia and that of fall down. These throwing techniques mention above, include the motion of the body rotation and the swing leg motion. The motion of the body rotation is visually clear, but to make it certain, we have examined upper body rotation by using an accelerometer and a gyroscope. The gyroscope showed the clear body rotation. We have compared this “Judo Health Exercise” to the traditional and widely spread “Radio Exercise No.1”. By this examination. we are able to conclude “Judo Health Exercise” is developed mainly for moving the lower part of the human body. On the other hand, “Radio Exevcise No.1” was developed mainly for moving the upper part of the human body. We have also examined the emotional difference between before practicing “Judo Health Exercise” after practicing it. For this examination, We have used a questionnaire. We have depicted the motion of “Judo Health Exercise”. In the future, we are planning “Judo Health Exercise” to spread throughout Japan.1520297128944766464application/pdfdepartmental bulletin pape