12 research outputs found
Singlet oxygen generation by photoactive polymeric microparticles with enhanced aqueous compatibility
Influence of polymer composition on the sensitivity towards nitrite and nitric oxide of colorimetric disposable test strips
The influence of polymer composition on the
sensitivity towards nitrite (NO
2
−
) and nitric oxide (NO)
of a series of 19 polymeric hydrogel films has been stud-
ied. The polymers, based on the hydrophilic monomer 2-
hydroxyethylmethacrylate (HEMA), are able to encapsu-
late the colorimetric indicator 1,2-diaminoanthraquinone
(DAQ) and to respond to NO
2
−
and NO by visual chang-
es. In the case of nitrite, the calculated limits of detection
(LOD) for two of the polymeric sensors (10
μ
M) are
very close to the sensitivity estimated for free DAQ in
solution (LOD 5
μ
M), but with the advantage of a solid
supported sensor with the format of a disposable
test-
strip
made with affordable starting chemicals. The re-
sults are interpreted taking
into account the nature and
proportions of monomers and cross-linkers used for the
synthesis of polymers. Key factors for obtaining sensi-
tive materials are the hydro
philic character of the film
along with the utilization of low levels of cross-linker
and the use of an acidic monomer, like acrylic acid, as
a building block.Financial support from the Spanish MINECO
(CTQ2015-68429-R) and Fundació Caixa Castelló-UJI (P1·1B2015-76)
is acknowledged. V. F. thanks the financial support from UJI (predoctoral
fellowship). We thank SCIC/UJI for technical assistance
Modification of silica using piperazine for immobilization of palladium nanoparticles: a study of its catalytic activity as an efficient heterogeneous catalyst for Heck and Suzuki reactions
A convenient synthesis of novel 5-arylidene-2-imino-4-thiazolidinones using base supported ionic liquid-like phase (SILLP) as efficient green catalyst
Influence of polymer composition on the sensitivity towards nitrite and nitric oxide of colorimetric disposable test strips
Catalytic activity of copper-bis(oxazoline) grafted on mesoporous silica in enantioselective cyclopropanation
A photoinduced electron transfer-based nanoprobe as a marker of acidic organelles in mammalian cells
Photoinduced electron transfer (PET)-based molecular probes have been successfully used for the intracellular imaging of the pH of acidic organelles. In this study, we describe the synthesis and characterization of a novel PET-based pH nanoprobe and its biological application for the signaling of acidic organelles in mammalian cells. A fluorescent ligand sensitive to pH via the PET mechanism that incorporates a thiolated moiety was synthesized and used to stabilize gold nanoparticles (2.4?±?0.6 nm), yielding a PET-based nanoprobe. The PET nanoprobe was unambiguously characterized by transmission electron microscopy, proton nuclear magnetic resonance, Fourier transform infrared, ultraviolet-visible absorption, and steady-state/time-resolved fluorescence spectroscopies which confirmed the functionalization of the gold nanoparticles with the PET-based ligand. Following a classic PET behavior, the fluorescence emission of the PET-based nanoprobe was quenched in alkaline conditions and enhanced in an acidic environment. The PET-based nanoprobe was used for the intracellular imaging of acidic environments within Chinese hamster ovary cells by confocal laser scanning microscopy. The internalization of the nanoparticles by the cells was confirmed by confocal fluorescence images and also by recording the fluorescence emission spectra of the intracellular PET-based nanoprobe from within the cells. Co-localization experiments using a marker of acidic organelles, LysoTracker Red DND-99, and a marker of autophagosomes, GFP-LC3, confirm that the PET-based nanoprobe acts as marker of acidic organelles and autophagosomes within mammalian cells
Bridging the equity gap in patient education: the biliary tract cancer BABEL project
No abstract available