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Root distribution and water use in coffee shaded with Tabebuia rosea Bertol. and Simarouba glauca DC. compared to full sun coffee in sub-optimal environmental conditions
Trypanosoma cruzi TcSMUG L-surface Mucins Promote Development and Infectivity in the Triatomine Vector Rhodnius prolixus
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Previous issue date: 2013Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq). Brasil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil.Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq). Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de BioquÃmica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Norte Fluminense - Horto. Centro de Biocieˆncias e Biotecnologia. Laborato´ rio de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM, CNPq). Brasil.Universidad Nacional de San MartÃn (UNSAM) - Consejo Nacional de Investigaciones CientÃficas y Técnicas (CONICET). Instituto de Investigaciones Biotecnológicas ‘‘Dr Rodolfo Ugalde’’. Campus UNSAM. Buenos Aires, Argentina.Universidad Nacional de San MartÃn (UNSAM) - Consejo Nacional de Investigaciones CientÃficas y Técnicas (CONICET). Instituto de Investigaciones Biotecnológicas ‘‘Dr Rodolfo Ugalde’’. Campus UNSAM. Buenos Aires, Argentina.nstituto Nacional de Entomologia Molecular (INCT-EM, CNPq). Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de BioquÃmica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Universidad Nacional de San MartÃn (UNSAM) - Consejo Nacional de Investigaciones CientÃficas y Técnicas (CONICET). Instituto de Investigaciones Biotecnológicas ‘‘Dr Rodolfo Ugalde’’. Campus UNSAM. Buenos Aires, Argentina.Background: TcSMUG L products were recently identified as novel mucin-type glycoconjugates restricted to the surface of
insect-dwelling epimastigote forms of Trypanosoma cruzi, the etiological agent of Chagas disease. The remarkable
conservation of their predicted mature N-terminal region, which is exposed to the extracellular milieu, suggests that
TcSMUG L products may be involved in structural and/or functional aspects of the interaction with the insect vector.
Methodology and Principal Findings: Here, we investigated the putative roles of TcSMUG L mucins in both in vivo
development and ex vivo attachment of epimastigotes to the luminal surface of the digestive tract of Rhodnius prolixus. Our
results indicate that the exogenous addition of TcSMUG L N-terminal peptide, but not control T. cruzi mucin peptides, to the
infected bloodmeal inhibited the development of parasites in R. prolixus in a dose-dependent manner. Pre-incubation of
insect midguts with the TcSMUG L peptide impaired the ex vivo attachment of epimastigotes to the luminal surface
epithelium, likely by competing out TcSMUG L binding sites on the luminal surface of the posterior midgut, as revealed by
fluorescence microscopy.
Conclusion and Significance: Together, these observations indicate that TcSMUG L mucins are a determinant of both
adhesion of T. cruzto the posterior midgut epithelial cells of the triatomine, and the infection of the insect
vector, R. prolixus