13 research outputs found
Energy- and flux-budget turbulence closure model for stably stratified flows. Part II: the role of internal gravity waves
We advance our prior energy- and flux-budget turbulence closure model
(Zilitinkevich et al., 2007, 2008) for the stably stratified atmospheric flows
and extend it accounting for additional vertical flux of momentum and
additional productions of turbulent kinetic energy, turbulent potential energy
(TPE) and turbulent flux of potential temperature due to large-scale internal
gravity waves (IGW). Main effects of IGW are following: the maximal value of
the flux Richardson number (universal constant 0.2-0.25 in the no-IGW regime)
becomes strongly variable. In the vertically homogeneous stratification, it
increases with increasing wave energy and can even exceed 1. In the
heterogeneous stratification, when IGW propagate towards stronger
stratification, the maximal flux Richardson number decreases with increasing
wave energy, reaches zero and then becomes negative. In other words, the
vertical flux of potential temperature becomes counter-gradient. IGW also
reduce anisotropy of turbulence and increase the share of TPE in the turbulent
total energy. Depending on the direction (downward or upward), IGW either
strengthen or weaken the total vertical flux of momentum. Predictions from the
proposed model are consistent with available data from atmospheric and
laboratory experiments, direct numerical simulations and large-eddy
simulations.Comment: 37 pages, 5 figures, revised versio
Methylated H3K4, a Transcription-Associated Histone Modification, Is Involved in the DNA Damage Response Pathway
Eukaryotic genomes are associated with a number of proteins such as histones that constitute chromatin. Post-translational histone modifications are associated with regulatory aspects executed by chromatin and all transactions on genomic DNA are dependent on them. Thus, it will be relevant to understand how histone modifications affect genome functions. Here we show that the mono ubiquitylation of histone H2B and the tri-methylation of histone H3 on lysine 4 (H3K4me3), both known for their involvement in transcription, are also important for a proper response of budding yeast cells to DNA damaging agents and the passage through S-phase. Cells that cannot methylate H3K4 display a defect in double-strand break (DSB) repair by non-homologous end joining. Furthermore, if such cells incur DNA damage or encounter a stress during replication, they very rapidly lose viability, underscoring the functional importance of the modification. Remarkably, the Set1p methyltransferase as well as the H3K4me3 mark become detectable on a newly created DSB. This recruitment of Set1p to the DSB is dependent on the presence of the RSC complex, arguing for a contribution in the ensuing DNA damage repair process. Taken together, our results demonstrate that Set1p and its substrate H3K4me3, which has been reported to be important for the transcription of active genes, also plays an important role in genome stability of yeast cells. Given the high degree of conservation for the methyltransferase and the histone mark in a broad variety of organisms, these results could have similar implications for genome stability mechanisms in vertebrate and mammalian cells
An example of enhanced tephra deposition driven by topographically induced atmospheric turbulence
Constitutive activation of a plasma membrane H+-ATPase prevents abscisic acid-mediated stomatal closure
Light activates proton (H+)-ATPases in guard cells, to drive hyperpolarization of the plasma membrane to initiate stomatal opening, allowing diffusion of ambient CO2 to photosynthetic tissues. Light to darkness transition, high CO2 levels and the stress hormone abscisic acid (ABA) promote stomatal closing. The overall H+-ATPase activity is diminished by ABA treatments, but the significance of this phenomenon in relationship to stomatal closure is still debated. We report two dominant mutations in the OPEN STOMATA2 (OST2) locus of Arabidopsis that completely abolish stomatal response to ABA, but importantly, to a much lesser extent the responses to CO2 and darkness. The OST2 gene encodes the major plasma membrane H+-ATPase AHA1, and both mutations cause constitutive activity of this pump, leading to necrotic lesions. H+-ATPases have been traditionally assumed to be general endpoints of all signaling pathways affecting membrane polarization and transport. Our results provide evidence that AHA1 is a distinct component of an ABA-directed signaling pathway, and that dynamic downregulation of this pump during drought is an essential step in membrane depolarization to initiate stomatal closure