D-galaktoz ile oluşturulmuş yaşlanma modelinde doku faktörü aktivitesinin ve sialik asit miktarının değerlendirilmesi

D-galaktoz ile oluşturulmuş yaşlanma modelinde doku faktörü aktivitesinin ve sialik asit miktarının değerlendirilmes

Early mobilisation in critically ill COVID-19 patients: a subanalysis of the ESICM-initiated UNITE-COVID observational study

Background Early mobilisation (EM) is an intervention that may improve the outcome of critically ill patients. There is limited data on EM in COVID-19 patients and its use during the first pandemic wave. Methods This is a pre-planned subanalysis of the ESICM UNITE-COVID, an international multicenter observational study involving critically ill COVID-19 patients in the ICU between February 15th and May 15th, 2020. We analysed variables associated with the initiation of EM (within 72 h of ICU admission) and explored the impact of EM on mortality, ICU and hospital length of stay, as well as discharge location. Statistical analyses were done using (generalised) linear mixed-effect models and ANOVAs. Results Mobilisation data from 4190 patients from 280 ICUs in 45 countries were analysed. 1114 (26.6%) of these patients received mobilisation within 72 h after ICU admission; 3076 (73.4%) did not. In our analysis of factors associated with EM, mechanical ventilation at admission (OR 0.29; 95% CI 0.25, 0.35; p = 0.001), higher age (OR 0.99; 95% CI 0.98, 1.00; p ≤ 0.001), pre-existing asthma (OR 0.84; 95% CI 0.73, 0.98; p = 0.028), and pre-existing kidney disease (OR 0.84; 95% CI 0.71, 0.99; p = 0.036) were negatively associated with the initiation of EM. EM was associated with a higher chance of being discharged home (OR 1.31; 95% CI 1.08, 1.58; p = 0.007) but was not associated with length of stay in ICU (adj. difference 0.91 days; 95% CI − 0.47, 1.37, p = 0.34) and hospital (adj. difference 1.4 days; 95% CI − 0.62, 2.35, p = 0.24) or mortality (OR 0.88; 95% CI 0.7, 1.09, p = 0.24) when adjusted for covariates. Conclusions Our findings demonstrate that a quarter of COVID-19 patients received EM. There was no association found between EM in COVID-19 patients' ICU and hospital length of stay or mortality. However, EM in COVID-19 patients was associated with increased odds of being discharged home rather than to a care facility. Trial registration ClinicalTrials.gov: NCT04836065 (retrospectively registered April 8th 2021)

AST, ALT, GGT, ALP, CK activities in serum, liver and kidney tissues in lectin applied normal mice and mice with tumour patient

Bu çalışma İstanbul Üniversitesi Araştırma Fonu’nca desteklenmiştir (Proje No:T-339/270697).Bu çalışmada, antikarsinojen bir madde olarak da kullanılan Viscum album Aglutinin (VAA)’nın doz aşımı uygulamalarının serum, karaciğer ve böbrek aspartat transaminaz (AST), alanin transaminaz (ALT), gamaglutamiltransferaz (GGT), alkalen fosfataz (ALP) ve kreatin kinaz (CK) enzim aktiviteleri üzerine olan etkisinin ortaya çıkarılması amaçlanmıştır. Çalışmada deneme materyali olarak, 80 adet BALB/c erkek fare kullanılmış ve dört eşit gruba ayrılmıştır. Donör bir fareden 0,1ml (1,2x106 ) Erlich ascites hücresi alınmış ve bu hücreler kanserli kontrol ve lektin deneme grubuna periton içi (i.p.) olarak uygulanmıştır. Uygulama günü 0. gün olarak kabul edilmiştir. Aynı gün, normal kontrol ve lektin kontrol gruplarına 0,1 ml. serum fizyolojik i.p. olarak verilmiştir. Lektin kontrol ve lektin deneme grubuna, 1. ve 2. günlerde 1ml/fare (100ng/fare) VAA verilirken, normal kontrol ve kanserli kontrol gruplarına serum fizyolojik verilmiştir. Tüm gruplardan, kan örnekleri 2., 7. ve 15. günlerde göz venasından kapillar tüpler aracılığı ile serum tüplerine alınmıştır. Tüm gruplardan, 2. günde 5 hayvan, 7. günde 5 hayvan, 15. günde ise 10 hayvan otopsiye gönderilmiştir. Otopside, farelerden karaciğer ve böbrek örnekleri alınmıştır. Serum, karaciğer ve böbrek üst sıvısında AST, ALT, GGT, ALP, CK enzim aktiviteleri ölçülmüştür. Biyokimyasal analizlerin sonucunda, kontrol grublarına göre lektin deneme grubunda serum AST, ALT, GGT, ALP ve CK aktiviteleri yükselmiştir. Tüm kontrol gruplarına göre lektin deneme grubunda karaciğer AST, ALT, GGT ve ALP spesifik aktiviteleri yükselirken, CK spesifik aktivitesi değişmemiştir. Kontrol gruplarına göre lektin deneme grubunda böbrek AST, ALT, GGT ve ALP spesifik aktiviteleri yükselirken, kanserli kontrol grubuna göre lektin deneme grubundaki enzim spesifik aktivitelerinde anlamlı değişimler bulunamamıştır. Enzim aktivitelerindeki bu artışların VAA’nın toksik etkisinden kaynaklanabileceği şeklinde yorumlanmıştırThe aim of the study was observed the effect of over dose application of Viscum album Agglutinine (VAA) which is used as a antikarsinogenic agent on the activities of aspartate aminotransferase (AST), alkaline aminotransferase (ALT), gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP) and creatine kinase (CK) enzymes in serum, liver and kidney. As experimental material, 80 BALB/c male mice were used and divided into four equal groups. 0,1ml (1,2x106 ) Erlich ascites cells were taken from a donor mouse and those cells were given cancer control and lectin trial groups by intraperitoneal (i.p.). Application day was accepted as day 0. In the same day, 0,1 ml serum physiologic was given normal control and lectin control groups by i.p. While 1ml/mause (100ng/mause) VAA was giving lectin control and lectin trial groups by i.p. on days 1 and 2. 0,1 ml/mouse serum physiologic was given normal control and lectin control groups. From whole groups, the blood samples were taken from ocular vena via capillary tubes into serum tubes on days 2, 7 and 15. 5 animals on days 2, 5 animals on days 7, 10 animals on day 15 were sent to autopsy. In the autopsy, samples of liver and kidney tissues were taken from mice. AST, ALT, GGT, ALP, CK enzymes activities were measured in serum and supernatant of liver and kidney. At the result of biochemical analyses, serum AST, ALT, GGT, ALP, CK activities increased in lectin trial group in comparison with whole control groups. While liver AST, ALT, GGT, ALP specific activities increased in lectin trial group in compare with whole control groups, CK specific activity did not change. While kidney AST, ALT, GGT, ALP specific activities increase in lectin trial group in comparison with normal control and lectin control groups, In the enzymes specific activities did not found significant chances in lectin trial group in compare with cancer control group. It was commended that the increase of enzymes activities can be from toxic effect of VA