Expression profile of genes involved in hydrogen sulphide liberation by _Saccharomyces cerevisiae_ grown under different nitrogen concentrations

The present work aims to elucidate molecular mechanisms underlying hydrogen sulphide production in _S. cerevisiae_ associated to nitrogen deficiency. To assess, at a genome-wide level, how the yeast strain adapted to the progressive nitrogen depletion and to nitrogen re-feeding, gene expression profiles were evaluated during fermentation at different nitrogen concentrations, using the DNA array technology. The results showed that most MET genes displayed higher expression values at the beginning of both control and N-limiting fermentation, just before the time at which the release of sulphide was observed. MET genes were downregulated when yeast stopped growing which could associate MET gene expression levels with cell growth. The over expression of MET genes after nitrogen addition was confirmed by a new release of H2S during the new set of fermentation experiments. In addition, to confirm gene expression profiles observed from macroarray results, real time RT-PCR was performed on 6 genes using additional sets of biological replicates. These genes were selected based on the assumption that differences in sulphide production observed among strains are due to genetic variations of the expression of genes involved in the Sulphate Reduction Pathway. An integration of expression data of genes involved in sulphur assimilation and sulphur amino acid biosynthesis with hydrogen sulphide production is presented

Decolonising English Studies from the Semi-Periphery

This book investigates how decolonising the curriculum might work in English studies — one of the fields that bears the most robust traces of its imperial and colonial roots — from the perspective of the semi-periphery of the academic world- system. It takes the University of Lisbon as a point of departure to explore broader questions of how the field can be rethought from within, through Anglophone (post)coloniality and an institutional location in a department of English, while also considering forces from without, as the arguments in this book issue from a specific, liminal positionality outside the Anglosphere. The first half of the book examines the critical practice of and the political push for decolonising the university and the curriculum, advancing existing scholarship with this focus on semi-peripheral perspectives. The second half comprises two theoretically-informed and classroom-oriented case studies of adaptation of the literary canon, a part of model syllabi that are designed to raise awareness of and encourage an understanding of a global, pluriversal literary history.info:eu-repo/semantics/publishedVersio

Rostos da morte na era da técnica

Tendo em conta a ideia defendida por diversos teóricos, de que as civilizações se definem pelo modo como tratam os moribundos e os defuntos, a morte surge-nos como um indicador privilegiado de questionamento do contexto social, como um dos grandes reveladores das sociedades e das civilizações e um dos instrumentos mais importantes para o seu questionamento e a sua crítica. O Homem da antiguidade, que vivia num mundo impregnado de paganismo e de maravilhoso, detinha com a morte uma relação de proximidade que a partir daí parece ter deixado de conseguir. As mudanças, que durante séculos foram sendo graduais, conheceram, no século XX, uma grande celeridade, tendo a morte e o morrer sido revestidos de uma invisibilidade social que se tornou num dos traços mais marcantes da era moderna. Associados às mudanças operadas nas diversas estruturas sociais, os ideais que proliferam no ocidente a partir da segunda metade do século passado, afastaram o fim de vida e a morte para os bastidores da vida social. A emergência do novo ideal de felicidade que proliferou no Ocidente nas últimas décadas do século XX e a aceleração do ritmo da vida que a sociedade moderna conheceu (aceleração que não contempla interrupções, ritmo vertiginoso que se mostra indiferente à paragem definitiva a que a morte obriga), contribuíram para um certo silenciamento social de dimensões tão intrínsecas à existência quanto o sofrimento e a morte. Mas nos finais século XX, início do século XXI, o panorama parece ter voltado a mudar: o aparecimento de doenças de difícil ou impossível controlo que surgem ligadas à senescência e afectam um número importante de indivíduos, o aparecimento de novas doenças, as dificuldades com que a medicina se confronta no combate a doenças como o cancro, que se tornaram, sobretudo, doenças crónicas, com finais de vida muito prolongados exigindo um tipo de intervenção específica (em nome das quais a medicina paliativa se desenvolveu), bem como as acções terroristas, que, com os atentados 11 de Setembro de 2001, vieram pôr em causa a aparente intocabilidade do valor da vida do homem contemporâneo e a segurança que caracterizou os ideais da vida dos tempos modernos. Todos estes aspectos vieram dar uma nova visibilidade e conceptualização à morte dos dias de hoje

The Photographic Ateliers of Carlos Relvas

Photography studies have not always been dedicated to the analysis of photographic studios to better understand the works of great photographers. This essay focuses on the different photographic studios of Carlos Relvas (1838-1894) in Golegã, with a particular focus on the little-studied and littleknown transformations of his splendorous Photographic Atelier of Golegã into a residential space. In the 1870s, the paradigm shift of photosensitive emulsions to faster exposure times had an impact on the way Carlos Relvas looked at his studio, forcing him to rethink the investment made in a space that no longer corresponded to the new technical reality of photography. Understood as a light production machine, Carlos Relvas’ studio is a key piece in his photographic work. In this essay, through the analysis of his photographs, we recover details and aspects of his various studios that are revealing of his inventive spirit, as well as his desire to keep up with the evolution of photographic art.&nbsp

Introduction: Navigating with the Blackstar: the Mediality of David Bowie

Despite the death of David Jones, David Bowie continues to shine with a distinctive luminosity and navigational function. He shines in absence and in repetition in various venues, as on a wall in Turnpike Lane. Our approach to this special issue of Celebrity Studies is framed by the idea that a social, cultural, ideological and semiotic understanding of stardom is entirely fitting for an examination of David Bowie and his legacy. Building on previous research on Bowie’s star personae (Stevenson 2006, Cinque and Redmond 2014, Cinque et al. 2015), this framework can be further inflected by considering the cosmological significance of stars in relation to Bowie. For instance, stars provide constellations of perpetual guiding points which enable enlightened communities to navigate pathways. Stars also have a spectral dimension, since they are luminous, but not always visible. Stars might seem static and omnipresent, but they are constantly transforming, changing appearance, ageing, living and dying. As Bowie has so aptly emphasised in his lyrics and interviews, there are many different types of stars (including his contentious statement that ‘Adolf Hitler was the first pop star’), and it was important that he set the record straight about which type of star he is: ‘… not a film star… not a pop star… not a marvel star… not a gang-star… I’m a star’s star… I’m a black star’.info:eu-repo/semantics/publishedVersio

Nalini Malani: Utopia!? – Nalini Malani: A Arte da Citação Enquanto Ativismo

‘Nalini Malani: Utopia!?’, a retrospective of Nalini Malani’s animation artworks at the Serralves Museum (19 December 2020–29 August 2021), follows the development of Malani’s artistic path through a continued investment in ‘resonance’.1 As a retrospective, the exhibition includes works from the Indian artist’s first experimental colour stop-motion 8 mm animation film, Dream Houses (1969), produced at the Vision Exchange Workshop and transferred to digital medium, to the immersive, nine- channel video installation Can You Hear Me? (2017–2020). At the same time, the artworks on display document the imagining of India, probing the utopia of a nation, as reflected in the exclamation and interrogation points in the retrospective’s title. The artworks take visitors to the aftermath of political independence and partition in 1947 and the to-be-realisation of Gandhi’s utopian vision in Nehruvian modernity – the historical conjuncture and sentiment that inform Dream Houses. This essay assesses how quotation (and, generally, adaptation) functions in Malani’s artworks as a visual and acoustic way of creating resonance in the contemporary moment, thinking through ‘originals’ as a form of cultural activism.info:eu-repo/semantics/publishedVersio

Sintomatología musculoesquelética en estudiantes de enfermería: un análisis de concepto

Objective: to analyze the musculoskeletal symptomatology concept in undergraduate nursing students through Rodgers' evolutionary method. Method: An integrative review of the literature was performed for the identification and selection of ten articles. A concept analysis was performed according to Rodgers' evolutionary method. Article search was performed using the EBOSCO Host platform, Virtual Health Library and Google scholar for the years from 2004 to 2018. Results: Based on the 3 elements of Rodgers model we found: 1) substitute terms and related concepts, where musculoskeletal disorders and musculoskeletal symptoms were highlighted; 2) as essential attributes of the concept we highlight pain and discomfort, as well as, measurement using the Nordic Musculoskeletal Questionnaire; and 3) risk factors were identified as antecedents, and the consequences were the impact on students' lives. Conclusions: This study contributes to the knowledge and clarification of the concept of musculoskeletal symptomatology in nursing students