11 research outputs found
Analysis of free fractions for chiral drugs using ultrafast extraction and multi-dimensional high-performance affinity chromatography
Estudo empírico da degradação de óleo de soja correlacionado com a sua constante dielétrica utilizando um sensor capacitivo
Analysis of free drug fractions by ultrafast affinity extraction: Interactions of sulfonylurea drugs with normal or glycated human serum albumin
Seeking to shed some light on the binding of fluoroquinolones to albumins
Interactions between serum albumins (HSA, human, and BSA, bovine) and fluoroquinolones (FQs), such as enoxacin, norfloxacin, ciprofloxacin, and ofloxacin, have been studied using the laser flash photolysis technique. Lifetimes and quantum yields of FQs triplet excited states ((3)FQs) are not affected by the presence of albumins, however, the quenching of (3)FQs by tryptophan and tyrosine and the subsequent generation of FQs. radical anions and tyrosyl or tryptophanyl radicals were detected. These results, besides agreeing with association constants (K-a) for FQs binding to albumins lower than 6 x 10(2) M-1, are highly relevant to understanding the process of photohapten formation, the first event in the onset of photoallergy. The emission of tryptophan within albumin is not affected by the presence of FQs when the inner filter effects (IFE) of these drugs are taken into account, which explains the discrepancies reported in the literature about K-a of FQs with albumins.Financial support from Spanish government (CTQ2010-19909) is gratefully acknowledged.Bosca Mayans, F. (2012). Seeking to shed some light on the binding of fluoroquinolones to albumins. Journal of Physical Chemistry B. 116(11):3504-3511. https://doi.org/10.1021/jp208930qS350435111161