14 research outputs found
In vitro survival of in vitro-produced bovine embryos cryopreserved by slow freezing, fast freezing and vitrification
Is the early reduction of fetal calf serum concentration in bovine in vitro embryo culture beneficial?
Use of different pressures for transvaginal follicular aspiration in mares
ABSTRACT The success of transvaginal follicular aspiration in mares can be influenced by several factors, such as vacuum pump pressure levels. The present study aimed to investigate the effect of different negative pressures (150, 280 and 400mmHg) of the vacuum pump on the oocyte recovery in the mares. The mares (n=10) were undergoing follicular aspiration using three different negative pressures for three consecutive estrous cycles as follows: G150 = 150mmHg (n = 10); G280 = 280mmHg (n = 10); G400 = 400mmHg (n = 10). Every estrous cycle, the group that the mare would participate was drawn, and each animal participated once in each group. Only preovulatory follicle was used, about 30 to 36 hours after application of hCG. To compare the results, the chi-square test was used (5% significance) and Fisher exact test, when recommended. Thirty preovulatory follicles (diameter 36.1±1.80mm) were aspirated and ten oocytes were recovered (33.3%). There was no statistical difference between the experimental groups (p=0.59). Thus, accord to the results observed in this study, we could conclude that the negative pressure of the vacuum pump used was not efficient to increase oocyte recovery
113COMPARISON OF TWO ETHYLENE GLYCOL EQUILIBRATION TREATMENTS FOR THE QUICK FREEZING OF IN VITRO-PRODUCED BOVINE EMBRYOS
59IN VITRO AND IN VIVO SURVIVAL OF NELORE NUCLEAR TRANSFER EMBRYOS RECONSTRUCTED WITH ADULT AND FETAL FIBROBLASTS
Achados de ressonância magnética de metástase de rabdomiossarcoma para mama: Relato de caso e revisão da literatura
Reportamos um caso de uma paciente do sexo feminino, 17 anos, com grande massa mamária palpável e história prévia de rabdomiossarcoma orbitário diagnosticado dezoito meses antes. Após exames de imagem e biópsia percutânea, foi realizado o diagnóstico de metástase de rabdomiossarcoma do tipo alveolar
Controle biológico de antracnose em pós-colheita de banana “Maçã” com Saccharomyces spp.
Production of a cloned calf from a fetal fibroblast cell line
The present study examined the in vitro and in vivo development of bovine nuclear-transferred embryos. A bovine fetal fibroblast culture was established and used as nucleus donor. Slaughterhouse oocytes were matured in vitro for 18 h before enucleation. Enucleated oocytes were fused with fetal fibroblasts with an electric stimulus and treated with cytochalasin D and cycloheximide for 1 h followed by cycloheximide alone for 4 h. Reconstructed embryos were cultured for 7-9 days and those which developed to blastocysts were transferred to recipient cows. Of 191 enucleated oocytes, 83 (43.5%) were successfully fused and 24 (28.9%) developed to blastocysts. Eighteen freshly cloned blastocysts were transferred to 14 recipients, 5 (27.8%) of which were pregnant on day 35 and 3 (16.7%) on day 90. Of the three cows that reached the third trimester, one recipient died of hydrallantois 2 months before term, one aborted fetus was recovered at 8 months of gestation, and one delivered by cesarian section a healthy cloned calf. Today, the cloned calf is 15 months old and presents normal body development (378 kg) and sexual behavior (libido and semen characteristics)