19 research outputs found
Ceratoconjuntivite por Encephalitozoon hellem em periquitos agapornis (Agapornis spp.) no Brasil: relato de caso
Blood parameters and measurements of weight in the rehabilitation of Magellanic penguins (Spheniscus magellanicus, Foster 1781)
Características morfológicas e fitossanitárias de variedades de roseira na etapa de classificação
Molecular characterisation of Cryptosporidium spp. in lambs in the South Central region of the State of São Paulo
Fatores de risco associados à infecção por Cryptosporidium spp. e Giardia duodenalis em bovinos leiteiros na fase de cria e recria na mesorregião do Campo das Vertentes de Minas Gerais
Caracterização molecular de Cryptosporidium spp. em criações comerciais brasileiras de coelhos
RESUMO A criptosporidiose é uma importante zoonose que pode ser transmitida por meio de alimentos, água de bebida e por contato com animais e pessoas infectadas. Além disso, trata-se de uma enfermidade clínica ou subclínica frequente em diversas espécies de animais, incluindo coelhos domésticos. O objetivo deste estudo foi determinar a ocorrência de Cryptosporidium spp., realizar sua classificação molecular e relacionar a presença do parasito às diferentes fases de criação em 21 criações comerciais de coelhos, localizadas nos estados de Minas Gerais, Mato Grosso do Sul, Pernambuco, Paraná, Rio de Janeiro, Rio Grande do Sul e São Paulo. Quinhentas e catorze amostras de fezes foram colhidas e armazenadas em solução de dicromato de potássio 5%. Os oocistos foram purificados por centrífugo-flutuação em solução de Sheather e visualizados por microscopia, utilizando-se a coloração negativa com verde malaquita. Cinquenta e cinco amostras foram submetidas à reação em cadeia pela polimerase (nested PCR) e ao sequenciamento de fragmentos amplificados, referentes aos genes da subunidade 18S do rRNA e da glicloproteína GP60, visando à caracterização molecular de Cryptosporidium spp. Oito amostras foram positivas para Cryptosporidium spp. pela microscopia (1,56%; 8/514) e sete foram positivas pela nested PCR (12,73%; 7/55). Pela análise molecular, foi possível identificar Cryptosporidium cuniculus (18S rRNA) e C. cuniculus subtipo VbA21 (gp60) em coelhos jovens e em matrizes
Corrigendum: Dairy calves in Uruguay are reservoirs of zoonotic subtypes of Cryptosporidium parvum and Pose a potential risk of surface water contamination. (Frontiers in Veterinary Science, (2020), 7, (562), 10.3389/fvets.2020.00562)
A Corrigendum on "Dairy Calves in Uruguay Are Reservoirs of Zoonotic Subtypes of Cryptosporidium parvum and Pose a Potential Risk of SurfaceWater Contamination"by Caffarena, R. D., Meireles, M. V., Carrasco-Letelier, L., Picasso-Risso, C., Santana, B. N., Riet-Correa, F., et al. (2020). Front. Vet. Sci. 7:562. doi: 10.3389/fvets.2020.00562 In the original article, there was an error in the acknowledgments section. The names of three persons and a staff group were omitted.A correction has been made to the acknowledgments: We also thank María Laura Casaux, Carlos Schild, Martín Fraga, and the staff from the Plataforma de Investigación en Salud Animal of INIA for their technical assistance
Dairy calves in Uruguay are reservoirs of zoonotic subtypes of cryptosporidium parvum and Pose a potential risk of surface water contamination.
Abstract:Cryptosporidium parvum, a major cause of diarrhea in calves, is of concern given its zoonotic potential. Numerous outbreaks of human cryptosporidiosis caused by C. parvum genetic subtypes are reported yearly worldwide, with livestock or water being frequently identified sources of infection. Although cryptosporidiosis has been reported from human patients in Uruguay, particularly children, epidemiologic information is scant and the role of cattle as reservoirs of zoonotic subtypes of C. parvum has not been explored. In this study, we aimed to (a)-identify C. parvum subtypes infecting dairy calves in Uruguay (including potentially zoonotic subtypes), (b)-assess their association with calf diarrhea, (c)-evaluate their spatial clustering, and (d)-assess the distance of infected calves to surface watercourses draining the farmlands and determine whether these watercourses flow into public water potabilization plants. Feces of 255 calves that had tested positive for Cryptosporidium spp. by antigen ELISA were selected. Samples had been collected from 29 dairy farms in seven Uruguayan departments where dairy farming is concentrated and represented 170 diarrheic and 85 non-diarrheic calves. Selected samples were processed by nested PCRs targeting the 18S rRNA and gp60 genes followed by sequencing to identify C. parvum subtypes. Of seven C. parvum subtypes detected in 166 calves, five (identified in 143 calves on 28/29 farms) had been identified in humans elsewhere and have zoonotic potential. Subtype IIaA15G2R1 was the most frequent (53.6%; 89/166), followed by IIaA20G1R1 (24.1%; 40/166), IIaA22G1R1 (11.4%; 19/166), IIaA23G1R1 (3.6%; 6/166), IIaA17G2R1 (3%; 5/166), IIaA21G1R1 (2.4%; 4/166), and IIaA16G1R1 (1.8%; 3/166). There were no significant differences in the proportions of diarrheic and non-diarrheic calves infected with any of the C. parvum subtypes. Two spatial clusters were detected, one of which overlapped with Uruguay?s capital city and its main water potabilization plant (Aguas Corrientes), harvesting surface water to supply ~1,700,000 people. Infected calves on all farms were within 20?900 m of a natural surface watercourse draining the farmland, 10 of which flowed into six water potabilization plants located 9?108 km downstream. Four watercourses flowed downstream into Aguas Corrientes. Calves are reservoirs of zoonotic C. parvum subtypes in Uruguay and pose a public health risk
Ocorrência e caracterização molecular de Cryptosporidium em cordeiros Occurrence and molecular characterization of Cryptosporidium in lambs
The occurrence of Cryptosporidium in fecal samples of lambs was determined in order to test correlations among the type of animal raising, feces consistence, index of oocysts elimination, animal age, and molecular characterization of species and genotypes involved in this parasitism. A total of 460 fecal samples were collected from lambs during the first 30 days of life, in various screeds of 21 farms. All samples were analyzed using malachite green negative coloration stain under microscopy. For molecular characterization of Cryptosporidium, on positive samples under microscopy, a nested PCR protocol was used for amplification of fragmentts from 18S rRNA gene or actin gene. It was found 6.7% of animals expeling oocysts of Cryptosporidium in feces of eight farms, without statistics relation among the analyzed indexes. The species and genotype involved were C. parvum type A 18S rRNA, C. parvum type B 18S rRNA and cervid genotype, which present a zoonotic potential of disease