27 research outputs found
Testing Biochemistry Revisited: How In Vivo Metabolism Can Be Understood from In Vitro Enzyme Kinetics
A decade ago, a team of biochemists including two of us, modeled yeast glycolysis and showed that one of the most studied biochemical pathways could not be quite understood in terms of the kinetic properties of the constituent enzymes as measured in cell extract. Moreover, when the same model was later applied to different experimental steady-state conditions, it often exhibited unrestrained metabolite accumulation
Iron Behaving Badly: Inappropriate Iron Chelation as a Major Contributor to the Aetiology of Vascular and Other Progressive Inflammatory and Degenerative Diseases
The production of peroxide and superoxide is an inevitable consequence of
aerobic metabolism, and while these particular "reactive oxygen species" (ROSs)
can exhibit a number of biological effects, they are not of themselves
excessively reactive and thus they are not especially damaging at physiological
concentrations. However, their reactions with poorly liganded iron species can
lead to the catalytic production of the very reactive and dangerous hydroxyl
radical, which is exceptionally damaging, and a major cause of chronic
inflammation. We review the considerable and wide-ranging evidence for the
involvement of this combination of (su)peroxide and poorly liganded iron in a
large number of physiological and indeed pathological processes and
inflammatory disorders, especially those involving the progressive degradation
of cellular and organismal performance. These diseases share a great many
similarities and thus might be considered to have a common cause (i.e.
iron-catalysed free radical and especially hydroxyl radical generation). The
studies reviewed include those focused on a series of cardiovascular, metabolic
and neurological diseases, where iron can be found at the sites of plaques and
lesions, as well as studies showing the significance of iron to aging and
longevity. The effective chelation of iron by natural or synthetic ligands is
thus of major physiological (and potentially therapeutic) importance. As
systems properties, we need to recognise that physiological observables have
multiple molecular causes, and studying them in isolation leads to inconsistent
patterns of apparent causality when it is the simultaneous combination of
multiple factors that is responsible. This explains, for instance, the
decidedly mixed effects of antioxidants that have been observed, etc...Comment: 159 pages, including 9 Figs and 2184 reference
Enhanced 2,3-butanediol production from biodiesel-derived glycerol by engineering of cofactor regeneration and manipulating carbon flux in Bacillus amyloliquefaciens
DIFFERENTIAL RESPONSE OF SERUM LH IN HYPOGONADOTROPIC HYPOGONADISM AND DELAYED PUBERTY TO LH-RH STIMULATION BEFORE AND AFTER CLOMIPHENE CITRATE ADMINISTRATION
DIFFERENTIAL RESPONSE OF SERUM LH IN HYPOGONADOTROPIC HYPOGONADISM AND DELAYED PUBERTY TO LH-RH STIMULATION BEFORE AND AFTER CLOMIPHENE CITRATE ADMINISTRATION
DIFFERENTIAL RESPONSE OF SERUM LH IN HYPOGONADOTROPIC HYPOGONADISM AND DELAYED PUBERTY TO LH-RH STIMULATION BEFORE AND AFTER CLOMIPHENE CITRATE ADMINISTRATION
THE ASSESSMENT OF HYPOTHALAMIC PITUITARY MATURATION DURING PUBERTY WITH A COMBINED CLOMIPHENE CITRATE GNRH TEST IN BOYS
Can yeast glycolysis be understood in terms of <I>in vitro</I> kinetics of the constituent enzymes?
NatuurwetenskappeBiochemiePlease help us populate SUNScholar with the post print version of this article. It can be e-mailed to: [email protected]