Copolymères à base d'ADN : synthèse, auto-assemblage, applications

Au cours de ce travail, nous avons cherché à obtenir une librairie de copolymères à base d'ADN, en utilisant différentes stratégies de synthèse visant à coupler un oligonucléotise à des polymères (hydrophobes, diblocs). Différentes techniques d'analyse (DDL, MET, DNPA, Cryo-MET...) ont permis d'étudier et de caractériser les structures auto-assemblées en solution aqueuse de ces biohybrides. Nous avons montré que les nano-objets formés à partir de polymères tribloc pouvaient encapsuler les molécules hydrophobes. Nous avons également montré qu'en utilisant les propriétés de reconnaissance de l'oligonucléotide, il était possible d'adsorber les nanoparticules de copolymères d'ADN sur une surface et de décorer celles-ci de nanoparticules d'or. Ces différents résultats ouvrent la voie à diverses applications des nanohybrides.In this workk, we built a library of copolymers based on DNA. For that purpose, we used various strategies of synthesis to link polymers (hydrophobic, diblocs) with oligonucleotides. Different techniques (DLS, TEM, SANS, Cryo-TEM...) allowed us to study and characterize the self-assembled structures in aqueous solution of these biohybrids. We worked out the potential applications of these biohybrids. We have shown that tribloc nanoparticles can encapsulate hydrophobic molecules. Using oligonucleotide recognition, it was possible to adsorb DNA copolymers nanoparticles on a surface and decorate these of golden nanoparticles. These results pave the way to new applications of nanohybrids

Copolymères à base d'ADN (synthèse, auto-assemblage, applications)

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Biohybrid block copolymers: towards functional micelles and vesicles.

International audienceThis critical review covers the elaboration of micelles and vesicles made from block copolymers containing peptide or oligonucleotide blocks with a focus on recent developments toward responsive and functional assemblies (166 references)

Investigation of the reversibility of the unimer-to-aggregate transition in block copolymers by surface tension-measurements.

International audienceTwo DNA-block copolymers, poly(caprolactone)-DNA and poly(methyl metacrylate)-DNA, were synthesized by conjugation of a short single strand of DNA (12 or 22mer) to a single reactive group at one end of the synthetic polymer. These polymers self-assemble in water, without the need of any cosolvent, forming micelle-like aggregates that were imaged by TEM. The solution behavior of the bioconjugated polymers was investigated by surface tension measurements. In the direction of dilution, the surface tension was measured using a down-scaled Wilhelmy plate method. To proceed in the reverse direction (concentration), we measured the surface tension of a sessile drop during its evaporation. This latter method was firstly validated using ionic and non-ionic surfactants, including polymeric surfactants. It was then applied to investigate the unimer to micelles transition of the DNA-block copolymers. In all cases, a reversible transition was observed demonstrating the existence of a critical micellar concentration, close to 0.01 mmol L(-1) for all the conjugates. The CMC was only slightly influenced by the length of the hydrophilic DNA block