A Pseudospectral Approach to High Index DAE Optimal Control Problems

Historically, solving optimal control problems with high index differential algebraic equations (DAEs) has been considered extremely hard. Computational experience with Runge-Kutta (RK) methods confirms the difficulties. High index DAE problems occur quite naturally in many practical engineering applications. Over the last two decades, a vast number of real-world problems have been solved routinely using pseudospectral (PS) optimal control techniques. In view of this, we solve a "provably hard," index-three problem using the PS method implemented in DIDO, a state-of-the-art MATLAB optimal control toolbox. In contrast to RK-type solution techniques, no laborious index-reduction process was used to generate the PS solution. The PS solution is independently verified and validated using standard industry practices. It turns out that proper PS methods can indeed be used to "directly" solve high index DAE optimal control problems. In view of this, it is proposed that a new theory of difficulty for DAEs be put forth.Comment: 14 pages, 9 figure

The cell biology of receptor-mediated virus entry

The cell imposes multiple barriers to virus entry. However, viruses exploit fundamental cellular processes to gain entry to cells and deliver their genetic cargo. Virus entry pathways are largely defined by the interactions between virus particles and their receptors at the cell surface. These interactions determine the mechanisms of virus attachment, uptake, intracellular trafficking, and, ultimately, penetration to the cytosol. Elucidating the complex interplay between viruses and their receptors is necessary for a full understanding of how these remarkable agents invade their cellular hosts

Automated Derivation of Complex System Constraints from User Requirements

International Space Station (ISS) payload developers submit their payload science requirements for the development of on-board execution timelines. The ISS systems required to execute the payload science operations must be represented as constraints for the execution timeline. Payload developers use a software application, User Requirements Collection (URC), to submit their requirements by selecting a simplified representation of ISS system constraints. To fully represent the complex ISS systems, the constraints require a level of detail that is beyond the insight of the payload developer. To provide the complex representation of the ISS system constraints, HOSC operations personnel, specifically the Payload Activity Requirements Coordinators (PARC), manually translate the payload developers simplified constraints into detailed ISS system constraints used for scheduling the payload activities in the Consolidated Planning System (CPS). This paper describes the implementation for a software application, User Requirements Integration (URI), developed to automate the manual ISS constraint translation process

A comparison of the pyrolysis of olive kernel biomass in fluidised and fixed bed conditions

The use of thermogravimetric analysis to describe biomass kinetics often uses bench top thermogravimetric analyser (TGA) analysers which are only capable of low heating rates. The aim of this research was to compare experimental fast pyrolysis of Olive kernels in a bespoke laboratory thermogravimetric fluidised bed reactor (TGFBR) characterised by rapid heating rates at high flow rates, compared to a smaller bench scale fixed bed TGA system. The pyrolysis in the TGFBR was analysed by using the isothermal kinetic approach and it was theorised that the pyrolysis decomposition reactions occurred by two mechanisms depending on the temperature, resulting in an activation energy of 67.4 kJ/mol at temperatures below 500 °C. For comparison, a bench scale TGA was used to look at the thermal behaviour in different fixed bed thermal conditions giving a higher activation energy of 74.4 kJ/mol due to the effect of external particle gas diffusion. The effect of biomass particle size (0.3–4.0 mm) on the conversion of biomass at different temperatures, was investigated between 300 and 660 °C in the TGFBR. The results suggested inhibition of internal gas diffusion was more important at lower temperatures, but in comparison had no significant effect when measured in the fixed bed TGA at lower heating rates. Bench top TGA analysis of pyrolysis is a rapid and valuable method, but is limited by smaller sample sizes and lower heating rates. In comparison, the conditions encountered with the laboratory scale TGFBR are more likely to be relevant to larger scale systems where heat distribution, heat transfer and mass diffusion effects play major roles in the reactivity of biomass

Infectious HIV-1 assembles in late endosomes in primary macrophages

Although human immunodeficiency virus type 1 (HIV-1) is generally thought to assemble at the plasma membrane of infected cells, virions have been observed in intracellular compartments in macrophages. Here, we investigated virus assembly in HIV-1–infected primary human monocyte-derived macrophages (MDM). Electron microscopy of cryosections showed virus particles, identified by their morphology and positive labeling with antibodies to the viral p17, p24, and envelope proteins, in intracellular vacuoles. Immunolabeling demonstrated that these compartments contained the late endosomal marker CD63, which was enriched on vesicles within these structures and incorporated into the envelope of budding virions. The virus-containing vacuoles were also labeled with antibodies against LAMP-1, CD81, and CD82, which were also incorporated into the viral envelope. To assess the cellular source of infectious viruses derived from MDM, virus-containing media from infected cells were precipitated with specific antibodies. Only antibodies against antigens found in late endosomes precipitated infectious virus, whereas antibodies against proteins located primarily on the cell surface did not. Our data indicate that most of the infectious HIV produced by primary macrophages is assembled on late endocytic membranes and acquires antigens characteristic of this compartment. This notion has significant implications for understanding the biology of HIV and its cell–cell transmission

Final Calibration of the Berkeley Extreme and Far-Ultraviolet Spectrometer on the ORFEUS-SPAS I and II Missions

The Berkeley Extreme and Far-Ultraviolet Spectrometer (BEFS) flew as part of the ORFEUS telescope on the ORFEUS-SPAS I and II space-shuttle missions in 1993 and 1996, respectively. The data obtained by this instrument have now entered the public domain. To facilitate their use by the astronomical community, we have re-extracted and re-calibrated both data sets, converted them into a standard (FITS) format, and placed them in the Multimission Archive at Space Telescope (MAST). Our final calibration yields improved wavelength scales and effective-area curves for both data sets.Comment: To appear in the January 2002 issue of the PASP. 17 pages with 9 embedded postscript figures; uses emulateapj5.st

Stakeholder engagement in the governance of marine migratory species: barriers and building blocks

Meaningful stakeholder engagement is important to collaborative decision-making and to effective polycentric governance, particularly when managing cross-scale environmental issues like those involving marine migratory species. In this paper, we explore the barriers to, and opportunities for, stakeholder involvement in the governance of threats to marine migratory species in eastern Australia, using semi-structured qualitative interviews and a focus group, as an example of the generic problem of managing migratory species within a large range state with multiple jurisdictions. Respondents identified several barriers to, and opportunities for, improved stakeholder involvement in the governance of marine migratory species, corresponding to 4 main themes: decision-making processes, information sharing, institutional structures, and participation processes. Respondents indicated that the governance system protecting marine turtles, dugongs, humpback whales, and non-threatened migratory shorebirds in eastern Australia would benefit from the introduction of new information pathways, reformed institutional structures (including environmental legislation), and improved participatory pathways for non-government stakeholders. Such changes could help harmonise the process of managing these species, leading to more effective conservation management throughout their range

Influence of clay minerals and associated minerals in alkali activation of soils

Alkali activation is promising for low environmental impact soil stabilisation. Given soils’ complexity, there is a lack of fundamental understanding of how the different components in soil influence their alkali activation behaviour. A novel method was developed to compare three natural soils with synthetic versions. Precursors and products were characterised by XRD, SEM, TGA and FTIR to explore the soils’ alkali activation phase formation behaviour. It is shown that only the clay minerals will determine phase formation, whereas most associated minerals had negligible influence. The trade-off between Na:Al and NaOH concentration in mix design means lower plasticity soils are more suitable

Corporate Bankruptcy Panel

Many municipalities all over the country are experiencing financial distress, and I believe this chapter of the bankruptcy system will be, and is currently being, tested tremendously by this crisis over the next years. There are a number of municipalities that are in bankruptcy now and, I believe, more to come. Whether the bankruptcy system can help solve that problem for all concerned, time will tell