Cytoplasmic genome substitution in wheat affects the nuclear-cytoplasmic cross-talk leading to transcript and metabolite alterations

Abstract Background Alloplasmic lines provide a unique tool to study nuclear-cytoplasmic interactions. Three alloplasmic lines, with nuclear genomes from Triticum aestivum and harboring cytoplasm from Aegilops uniaristata, Aegilops tauschii and Hordeum chilense, were investigated by transcript and metabolite profiling to identify the effects of cytoplasmic substitution on nuclear-cytoplasmic signaling mechanisms. Results In combining the wheat nuclear genome with a cytoplasm of H. chilense, 540 genes were significantly altered, whereas 11 and 28 genes were significantly changed in the alloplasmic lines carrying the cytoplasm of Ae. uniaristata or Ae. tauschii, respectively. We identified the RNA maturation-related process as one of the most sensitive to a perturbation of the nuclear-cytoplasmic interaction. Several key components of the ROS chloroplast retrograde signaling, together with the up-regulation of the ROS scavenging system, showed that changes in the chloroplast genome have a direct impact on nuclear-cytoplasmic cross-talk. Remarkably, the H. chilense alloplasmic line down-regulated some genes involved in the determination of cytoplasmic male sterility without expressing the male sterility phenotype. Metabolic profiling showed a comparable response of the central metabolism of the alloplasmic and euplasmic lines to light, while exposing larger metabolite alterations in the H. chilense alloplasmic line as compared with the Aegilops lines, in agreement with the transcriptomic data. Several stress-related metabolites, remarkably raffinose, were altered in content in the H. chilense alloplasmic line when exposed to high light, while amino acids, as well as organic acids were significantly decreased. Alterations in the levels of transcript, related to raffinose, and the photorespiration-related metabolisms were associated with changes in the level of related metabolites. Conclusion The replacement of a wheat cytoplasm with the cytoplasm of a related species affects the nuclear-cytoplasmic cross-talk leading to transcript and metabolite alterations. The extent of these modifications was limited in the alloplasmic lines with Aegilops cytoplasm, and more evident in the alloplasmic line with H. chilense cytoplasm. We consider that, this finding might be linked to the phylogenetic distance of the genomes.The authors thank Prof. S.S. Maan (North Dakota State University, USA) for providing the alloplasmic lines T183 and T195 and Dr. Alessio Aprile (University of Salento, Lecce, Italy) for help in data analysis. LQ was partly supported by grant # 2770367, the Chief Scientist Fund, the Ministry of Agriculture, Israel.Peer Reviewe

Building a politics of connectivity: intercultural in-commonness in Fairtrade

Fairtrade operates its global system through a homogenising but marketable set of standards. Combined with issues around how to include producers in governance, this has led to feelings of disconnection and disenfranchisement for the latter, which are impacting on Fairtrade’s effectiveness and legitimacy. Through a focus on the South African wine industry, this paper argues that the Fairtrade community needs to be reinvigorated through dialogical communication, impactful participation and cultural synthesis to better enact responsibility across its systemic geographical and cultural distances. “Being‐with” its multiple stakeholders makes space for a more responsive, contextual and connected system. Drawing on the ideas of Paulo Freire, the paper concludes that a Fairtrade built on solidarity through a participatory and decentralised system would allow for discussions of the ideals and practices that are essential to negotiating, and not swallowing up, the shifting “we” of Fairtrade and more effectively balancing its local and global responsibilities

From 'One Namibia, One Nation' towards 'Unity in Diversity? Shifting representations of culture and nationhood in Namibian Independence Day celebrations, 1990-2010

In 2010 Namibia celebrated its twentieth anniversary of independence from South African rule. The main celebrations in the country’s capital Windhoek became the stage for an impressively orchestrated demonstration of maturing nationhood, symbolically embracing postcolonial policy concepts such as ‘national reconciliation’, ‘unity’ and ‘diversity’. At the same time, nation building in post-apartheid Namibia is characterised by a high degree of social and political fragmentation that manifests itself in cultural and/or ethnic discourses of belonging. Taking the highly significant independence jubilee as our vantage point, we map out a shift of cultural representations of the nation in Independence Day celebrations since 1990, embodied by the two prominent slogans of ‘One Namibia, one Nation’ and ‘Unity in Diversity’. As we will argue, the difficult and at times highly fragile postcolonial disposition made it necessary for the SWAPO government, as primary nation builder, to accommodate the demands of regions and local communities in its policy frameworks. This negotiation of local identifications and national belonging in turn shaped, and continues to shape, the performative dimension of Independence Day celebrations in Namibia.Web of Scienc

Transcriptomic and biochemical investigations support the role of rootstock-scion interaction in grapevine berry quality

Background In viticulture, rootstock genotype plays a critical role to improve scion physiology, berry quality and to adapt grapevine (Vitis viniferaL.) to different environmental conditions. This study aimed at investigating the effect of two different rootstocks (1103 Paulsen - P - and Mgt 101-14 - M) in comparison with not grafted plants - NGC - on transcriptome (RNA-seq and small RNA-seq) and chemical composition of berry skin inPinot noir, and exploring the influence of rootstock-scion interaction on grape quality. Berry samples, collected at veraison and maturity, were investigated at transcriptional and biochemical levels to depict the impact of rootstock on berry maturation. Results RNA- and miRNA-seq analyses highlighted that, at veraison, the transcriptomes of the berry skin are extremely similar, while variations associated with the different rootstocks become evident at maturity, suggesting a greater diversification at transcriptional level towards the end of the ripening process. In the experimental design, resembling standard agronomic growth conditions, the vines grafted on the two different rootstocks do not show a high degree of diversity. In general, the few genes differentially expressed at veraison were linked to photosynthesis, putatively because of a ripening delay in not grafted vines, while at maturity the differentially expressed genes were mainly involved in the synthesis and transport of phenylpropanoids (e.g. flavonoids), cell wall loosening, and stress response. These results were supported by some differences in berry phenolic composition detected between grafted and not grafted plants, in particular in resveratrol derivatives accumulation. Conclusions Transcriptomic and biochemical data demonstrate a stronger impact of 1103 Paulsen rootstock than Mgt 101-14 or not grafted plants on ripening processes related to the secondary metabolite accumulations in berry skin tissue. Interestingly, theMYB14gene, involved in the feedback regulation of resveratrol biosynthesis was up-regulated in 1103 Paulsen thus supporting a putative greater accumulation of stilbenes in mature berries

Comment on "Optical properties of six-member carbon ring organic liquids in the vacuum ultraviolet"

SCOPUS: le.jinfo:eu-repo/semantics/publishe

Education directed to entrepreneurship : a guidance concern

Summaries in Afrikaans and EnglishHedendaagse onderwys staan voor 'n omvattende voorligtings- en opleidingsopgawe om die jeug toe te rus om die arbeidsmark as entrepreneurs te betree. Onderwysers word toenemend voor die eis gestel om as fasiliteerders op te tree om leerlinge te begelei om beter by die eise van veranderende omstandighede, nuwe tegnologie en inligting aan te pas. In hierdie konteks vorm entrepreneurskapsgerigte onderwys een van die mees eietydse temas wat die onderwys moet aanspreek. Hoewel die voorsiening van entrepreneurskapsgerigte onderwys reeds in die nuwe Norme en standaarde vir onderwyseropleiding as 'n kruiskurrikulere aangeleentheid beskryf word, is die behoefte aan 'n omvattende entrepreneurskapsontwikkelingstrategie nog nie voldoende aangespreek nie. In 'n antwoord op hierdie leemte word 'n voorgestelde implementeringstrategie vir entrepreneurskapsgerigte onderwys in hierdie studie ontwikkel. In die verloop van die ondersoek moes daar deeglik rekening gehou word met aspekte soos: • die rol en betekenis van entrepreneurskap in die Suid-Afrikaanse skolekonteks; • faktore wat entrepreneuriese sukses of mislukking onderle; • die ontwikkeling van 'n positiewe entrepreneuriese ingesteldheid en entrepreneuriese vaardighede en • plaaslike en oorsese inisiatiewe om entrepreneurskap m die skoolkonteks te bevorder. In die empiriese ondersoek is die uitgangspunt gehuldig dat onderwysers se menings van deurslaggewende belang is om 'n entrepreneurskapsontwikkelingstrategie te rig. Onderwysers se menings is in elk van die volgende ondersoekvelde nagevors: • Onderwysers se toegerustheid om entrepreneurskapsgerigte onderwys aan leerlinge te voorsien; • Onderwysers se eie implementering van entrepreneurskapsgerigte onderwys; • Die behoefte by leerlinge aan entrepreneurskapsgerigte onderwys; • Onderwysers se geslaagdheid om entrepreneurskapsgerigte onderwys te implementeer; • Die aangewesenheid van entrepreneurskapsgerigte onderwys as 'n onderwyseropgawe. Die navorsingsresultate is aangewend om binne die raamwerk van die Suid-Afrikaanse skolekonteks en onderwysbeleid 'n strategie voor te stel om entrepreneurskapsgerigte onderwys te implementeer. Die onderwys kan hierdeur 'n aansienlike bydrae lewer om die jeug voor te berei om as volwaardige en verantwoordelike landsburgers hulle plek in die samelewing vol te staan.Education today stands before the comprehensive task to empower our youth through appropriate guidance and training to enter the job market as entrepreneurs. Teachers are increasingly put to the task to act as facilitators in their guidance of the youth to adapt more effectively to changing circumstances, new technology and information. Within this context, education directed to entrepreneurship forms one of the most timely issues that education should address. Although the provision of education directed to entrepreneurship has been described as a cross curricular concern within the new Norms and standards for teacher education it does not provide in the need for a comprehensive strategy to develop entrepreneurship in education. In an answer to this deficiency, this study is directed to develop a proposed implementation strategy to enhance entrepreneurship in education. In the course of this investigation it was needed to thouroughly account with concerns such as: • the role and significance of entrepreneurship within the South African school context; • factors which underlie entrepreneurial success or failure; • the development of a positive entrepreneurial attitude and entrepreneurial skills and • local and foreign initiatives to enhance entrepreneurship within the school context. In the empirical investigation the v1ew is held that teachers opm10ns is of crucial importance to give direction towards a strategy to enhance entrepreneurship. Teachers views are examened within each of the following fields of investigation: • Teachers empoweredness to provide education directed to entrepreneurship to students. • Teachers own implementation of education directed to entrepreneurship • Students need for education directed to entrepreneurship. • Teachers sufficiency to implement education directed to entrepreneurship. • The appropriateness of education directed to entrepreneurship as a teacher concern. The results of the research is applied to propose an implementation strategy to enhance education directed to entrepreneurship within the framework of the South African school context and policy of education. The conclusion was reached that educators can greatly contribute to prepare our youth so that they can take up their positions in the community as responsible and able citizens.Psychology of EducationD. Ed. (Psychology of Education

Different kinds of IP effects and laboratory measurements samples

Several kinds of Induced Polarization (IP) effects occur and will be reviewed in the present paper, namely electrosmosis effect, membrane polarization, MaxwellWagner effect and electrolytical polarization. All effects are based on different physical phenomena. The electrosmosis processes occur in all rocks/sediments. However the amount of double electric layer plays the major role. This phenomenon is described by HelmholtzSmoluhowsky equation and is linear. Decay constant of electrosmosis process is usually in range 10-6- 10-2 s and can be mostly observed on Transient Electro-Magnetic (TEM) signals. The membrane polarization is based on constrictivity of pore. When an electrical current flows through rocks containing channels and pores with different sizes, an excess/loss of ions accumulates at the boundaries. The homogeneous diffusion equation, with specified (nonlinear) boundary conditions, has been used for solving this problem. This type of polarization is non-linear, depends on applied current and may depend on current pulse length (in TEM method). Duration of membrane polarization can reach 10 s and more. Maxwell-Wagner model consists of isolated pores. The homogeneous diffusion equation also has been used for solving the problem of ions distribution in the pores. However the boundary conditions are linear. The ions move in the pores with constant velocity and accumulated the neighbour of the pore ends. Duration of process of redistribution is comparable with duration of the electro osmosis process. The pores with unallocated ions may be represented by an equivalent electrical circuit (a capacitor). The Cole-Cole or capacitor discharging formula can be used to describe the effect. Numerous laboratory measurements of different types of rocks and minerals and some field TEM data demonstrate different kinds of IP effects